Nuclease target site selection for maximizing on-target activity and minimizing off-target effects in genome editing

Molecular Therapy

Volumn 24, Issue 3, 2016, Pages 475-487

  Lee, Ciaran M ^a   Cradick, Thomas J ^b   Fine, Eli J ^c   Bao, Gang ^a  

^a Rice University   (United States)

^b CRISPR THERAPEUTICS   (United States)

^c Coyne Scientific ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEASE; ENDONUCLEASE; PROTEIN BINDING;

CRISPR CAS SYSTEM; DNA END JOINING REPAIR; DNA MODIFICATION; DOUBLE STRANDED DNA BREAK; ENZYME ACTIVITY; ENZYME SPECIFICITY; GENE ACTIVITY; GENE DELETION; GENE INSERTION; GENE TARGETING; HOMOLOGOUS RECOMBINATION; REVIEW; SIGNAL TRANSDUCTION; ANIMAL; BINDING SITE; BIOLOGY; GENE EDITING; GENOME; GENOMICS; HUMAN; METABOLISM; PROCEDURES; WEB BROWSER;

ANIMALS; BINDING SITES; COMPUTATIONAL BIOLOGY; CRISPR-CAS SYSTEMS; ENDONUCLEASES; GENE EDITING; GENE TARGETING; GENOME; GENOMICS; HUMANS; PROTEIN BINDING; WEB BROWSER;

EID: 84960407885     PISSN: 15250016     EISSN: 15250024     Source Type: Journal    
DOI: 10.1038/mt.2016.1     Document Type: Review

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