Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease

Nature Biotechnology

Volumn 32, Issue 7, 2014, Pages 677-683

  Kuscu, Cem ^a   Arslan, Sevki ^a,b   Singh, Ritambhara ^a,c   Thorpe, Jeremy ^a   Adli, Mazhar ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

^b PAMUKKALE UNIVERSITY   (Turkey)

^c University of Virginia   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEIC ACIDS;

BACKGROUND LEVEL; CHROMATIN IMMUNOPRECIPITATION; CLINICAL RESEARCH; ENDONUCLEASES; HIGH-THROUGHPUT SEQUENCING;

GENES;

CAS9 ENDONUCLEASE; ENDONUCLEASE; GUIDE RNA; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CATALYSIS; CHROMATIN; CHROMATIN IMMUNOPRECIPITATION; CONTROLLED STUDY; EMBRYO; ENZYME ACTIVITY; ENZYME BINDING; GENOME ANALYSIS; HEK293 CELL LINE; HIGH THROUGHPUT SEQUENCING; HUMAN; HUMAN CELL; INDEL MUTATION; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN RNA BINDING; RNA SEQUENCE;

BASE SEQUENCE; BINDING SITES; CELLS, CULTURED; CHROMOSOME MAPPING; CRISPR-CAS SYSTEMS; DEOXYRIBONUCLEASE I; EMBRYONIC STEM CELLS; GENE TARGETING; GENOME; HEK293 CELLS; HUMANS; MODELS, GENETIC; MOLECULAR SEQUENCE DATA;

EID: 84903545084     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.2916     Document Type: Article

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