Chimeric nucleases stimulate gene targeting in human cells

Science

Volumn 300, Issue 5620, 2003, Pages 763-

  Porteus, Matthew H ^a,b   Baltimore, David ^a  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; GREEN FLUORESCENT PROTEIN; NUCLEASE; ZINC;

GENE; TARGETING;

BINDING SITE; CHIMERA; DNA BINDING; DROSOPHILA; GENE EXPRESSION; GENE FUSION; GENE MUTATION; GENE TARGETING; GENETIC TRANSFECTION; GENOME; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; SHORT SURVEY; SOMATIC CELL GENETICS; STEM CELL; XENOPUS;

CELL LINE; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DIMERIZATION; DNA; GENE TARGETING; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MUTATION; NUCLEAR LOCALIZATION SIGNALS; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; TRANSFECTION; ZINC FINGERS;

EID: 0038523969     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1078395     Document Type: Short Survey

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12. We thank J. Pomerantz and S Cameron for their helpful comments on the manuscript, and E. Denney for excellent technical support. We thank C. Pabo and K. Joung for providing plasmid pUV5-GP-Z123, D. Carroll for providing pET15b:QQR1(L0), S Chandrasegaran for providing pET15b:QQR1(L18), and M. Jasin for providing PGK-Sce. M.H.P. was supported by the Physician's Postdoctoral Fellowship from the HHMI and the Career Development Award from the Burroughs-Wellcome Fund. Supported by NIH grant RO1-GM39458 (to D.B.).