Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors

Nature Biotechnology

Volumn 33, Issue 2, 2015, Pages 175-179

  Wang, Xiaoling ^a   Wang, Yebo ^a,b   Wu, Xiwei ^a   Wang, Jinhui ^a   Wang, Yingjia ^a,b   Qiu, Zhaojun ^a   Chang, Tammy ^a   Huang, He ^b   Lin, Ren Jang ^a   Yee, Jiing Kuan ^a  

^a CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

^b ZHEJIANG UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTECHNOLOGY;

INTEGRASE; LENTIVIRAL VECTOR; TARGET ACTIVITY; TARGET SITES;

GENES;

GUIDE RNA; INTEGRASE; INTEGRASE DEFECTIVE LENTIVIRAL VECTOR; LENTIVIRUS VECTOR; PUROMYCIN; SINGLE GUIDE RNA; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; TYROSINE AMINOTRANSFERASE; UNCLASSIFIED DRUG;

ABSENCE; ARTICLE; CRISPR CAS SYSTEM; GENETIC TRANSFECTION; INDEL MUTATION; PRIORITY JOURNAL; RNA BINDING; WISKOTT ALDRICH SYNDROME; ENZYMOLOGY; GENE VECTOR; GENETICS; HEK293 CELL LINE; HIGH THROUGHPUT SEQUENCING; HUMAN; HUMAN GENOME; LENTIVIRINAE; RNA EDITING;

CRISPR-CAS SYSTEMS; GENETIC VECTORS; GENOME, HUMAN; HEK293 CELLS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; INTEGRASES; LENTIVIRUS; RNA EDITING;

EID: 84923221641     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.3127     Document Type: Article

References (20)

1. Cong, L. et al. Science 339, 819-823 (2013).
2. Perez-Pinera, P. et al. Nat. Methods 10, 973-976 (2013).
3. Shalem, O. et al. Science 343, 84-87 (2014).
4. Mali, P. et al. Nat. Biotechnol. 31, 833-838 (2013).
5. Wang, T., Wei, J.J., Sabatini, D.M. & Lander, E.S. Science 343, 80-84 (2014).
6. Xiao, A. et al. Nucleic Acids Res. 41, e141 (2013).
7. Fu, Y. et al. Nat. Biotechnol. 31, 822-826 (2013).
8. Hsu, P.D. et al. Nat. Biotechnol. 31, 827-832 (2013).
9. Cho, S.W. et al. Genome Res. 24, 132-141 (2014).
10. Pattanayak, V., Ramirez, C.L., Joung, J.K. & Liu, D.R. Nat. Methods 8, 765-770 (2011).
11. Pattanayak, V. et al. Nat. Biotechnol. 31, 839-843 (2013).
12. Gabriel, R. et al. Nat. Biotechnol. 29, 816-823 (2011).
13. Osborn, M.J. et al. Mol. Ther. 21, 1151-1159 (2013).
14. Ran, F.A. et al. Cell 154, 1380-1389 (2013).
15. Lin, Y. et al. Nucleic Acids Res. 42, 7473-7485 (2014).
16. Mali, P. et al. Science 339, 823-826 (2013).
17. Doyle, E.L. et al. Nucleic Acids Res. 40, W117-W122 (2012).
18. Wang, X. et al. PLoS ONE 9, e93575 (2014).
19. Paruzynski, A. et al. Nat. Protoc. 5, 1379-1395 (2010).
20. Zhang, J., Kobert, K., Flouri, T.X. & Stamatakis, A. Bioinformatics 30, 614-620 (2014).