Efficient fdCas9 synthetic endonuclease with improved specificity for precise genome engineering

PLoS ONE

Volumn 10, Issue 7, 2015, Pages

  Aouida, Mustapha ^a   Eid, Ayman ^a   Ali, Zahir ^a   Cradick, Thomas ^b   Lee, Ciaran ^b   Deshmukh, Harshavardhan ^b   Atef, Ahmed ^a   Abusamra, Dina ^a   Gadhoum, Samah Zeineb ^a   Merzaban, Jasmeen ^a   Bao, Gang ^b   Mahfouz, Magdy ^a  

^a KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Saudi Arabia)

^b Emory University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CAS9 PROTEIN; DOUBLE STRANDED RNA; ENDONUCLEASE; FOKI ENDONUCLEASE; GUIDE RNA; PROTEIN VARIANT; UNCLASSIFIED DRUG; ENDODEOXYRIBONUCLEASE FOKI; HYBRID PROTEIN; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE;

ACCURACY; ANIMAL CELL; ARTICLE; BIOASSAY; CONTROLLED STUDY; DNA STRAND BREAKAGE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INACTIVATION; GENE TARGETING; GENETIC ENGINEERING; MAMMAL CELL; NONHUMAN; PROTEIN DOMAIN; RNA BINDING; CHEMISTRY; CRISPR CAS SYSTEM; DNA END JOINING REPAIR; ENZYME SPECIFICITY; GENETICS; HEK293 CELL LINE; HUMAN; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROCEDURES; PROTEIN ENGINEERING; RNA EDITING;

BASE SEQUENCE; CRISPR-CAS SYSTEMS; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA END-JOINING REPAIR; ENDONUCLEASES; GENETIC ENGINEERING; HEK293 CELLS; HUMANS; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; RNA EDITING; RNA, GUIDE; SUBSTRATE SPECIFICITY;

EID: 84941975665     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0133373     Document Type: Article

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