CRISPRscan: Designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

Nature Methods

Volumn 12, Issue 10, 2015, Pages 982-988

  Moreno Mateos, Miguel A ^a   Vejnar, Charles E ^a   Beaudoin, Jean Denis ^a   Fernandez, Juan P ^a   Mis, Emily K ^a   Khokha, Mustafa K ^a   Giraldez, Antonio J ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANINE; GUANINE QUADRUPLEX; GUIDE RNA; 3' UNTRANSLATED REGION; ADENINE; ZEBRAFISH PROTEIN;

5' UNTRANSLATED REGION; ANIMAL EXPERIMENT; ARTICLE; BASE MISPAIRING; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT CAS9; CONTROLLED STUDY; DNA DAMAGE; EMBRYO; GENE TARGETING; GERM LINE; HEART DEVELOPMENT; IN VITRO STUDY; IN VIVO STUDY; INDEL MUTATION; INDUCED MUTATION; MUTAGENIC ACTIVITY; NONHUMAN; NOTOCHORD; NUCLEOSOME; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION; RNA STRUCTURE; SOMATIC MUTATION; 3' UNTRANSLATED REGION; ALGORITHM; ANIMAL; CRISPR CAS SYSTEM; EMBRYOLOGY; FEMALE; GENE EXPRESSION REGULATION; GENETIC ENGINEERING; GENETICS; GENOME; MUTATION; NONMAMMALIAN EMBRYO; PROCEDURES; XENOPUS; ZEBRA FISH;

3' UNTRANSLATED REGIONS; ADENINE; ALGORITHMS; ANIMALS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; EMBRYO, NONMAMMALIAN; FEMALE; GENE EXPRESSION REGULATION; GENE TARGETING; GENETIC ENGINEERING; GENOME; GUANINE; MUTATION; RNA, GUIDE; XENOPUS; ZEBRAFISH; ZEBRAFISH PROTEINS;

EID: 84959123021     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.3543     Document Type: Article

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