An efficient and targeted gene integration system for high-level antibody expression

Journal of Immunological Methods

Volumn 322, Issue 1-2, 2007, Pages 28-39

  Huang, Ying ^a   Li, Yan ^a   Wang, Yu Gang ^a   Gu, Xin ^b   Wang, Yan ^b   Shen, Bei Fen ^a  

^a INSTITUTE OF BASIC MEDICAL SCIENCES   (China)

^b Beijing Development Area   (China)

Author keywords

Engineered cell line; Fluorescence in situ hybridization; FRT FLP; High level producer; Recombinant antibody; Site specific recombination

Indexed keywords

DIHYDROFOLATE REDUCTASE; GALACTOSIDASE; HYGROMYCIN; METHOTREXATE;

ANIMAL CELL; ANIMAL TISSUE; ANTIBODY DETECTION; ARTICLE; CONTROLLED STUDY; FLUORESCENCE IN SITU HYBRIDIZATION; GENE AMPLIFICATION; GENE EXPRESSION REGULATION; GENE LOCATION; GENE LOCUS; GENE TARGETING; GENETIC STABILITY; GENETIC TRANSFECTION; NONHUMAN; POSITION EFFECT; PRIORITY JOURNAL; RANDOM INTEGRATION; SOUTHERN BLOTTING; TRANSCRIPTION REGULATION;

ANIMALS; ANTIBODIES; CHO CELLS; CRICETINAE; CRICETULUS; DNA; FEMALE; GENE AMPLIFICATION; GENE EXPRESSION; GENE TARGETING; GENETIC ENGINEERING; IN SITU HYBRIDIZATION, FLUORESCENCE; PLASMIDS; RECOMBINANT PROTEINS; TRANSCRIPTION, GENETIC;

CRICETULUS GRISEUS;

EID: 34147179033     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2007.01.022     Document Type: Article

References (30)

1. Alami R., Greally J.M., Tanimoto K., Hwang S., Feng Y.Q., Engel J.D., Fiering S., and Bouhassira E.E. The b-Globin LCR controls expression levels but does not prevent heterocellular position effects. Hum. Mol. Genet. 9 (2000) 631
2. Bacsi S.G., and Wejksnora P.J. Effect of increase in ploidy on the activation of nucleolar organizer regions in Chinese hamster ovary (CHO) cells. Exp. Cell Res. 165 (1986) 283
3. Bebbington C.R., Renner G., Thomson S., King D., Abrams D., and Yarranton G.T. High level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as amplifiable selectable marker. Biotechnology 10 (1992) 169
4. Bode J., Schlake T., Iber M., Schübeler D., Seibler J., Snezhkov E., and Nikolaev L. The transgeneticists toolbox: novel methods for the targeted modification of eukaryotic genomes. Biol. Chem. 381 (2000) 801
5. Bode J., Fetzer C.P., Nehlsen K., Scinteie M., Hinrichs B.H., Baiker A., Piechaczek C., Benham C., and Lipps H.J. The Hitchhiking Principle: optimizing episomal vectors for the use in gene therapy and biotechnology. Gene Ther. Mol. Biol. 6 (2001) 33
6. Chomczynski P. One-hour downward alkaline capillary transfer for blotting of DNA and RNA. Anal. Biochem. 201 (1992) 134
7. Deaven L.L., and Petersen D.F. The chromosomes of CHO, an aneuploid Chinese hamster cell line: G-band, C-band, and autoradiographic analyses. Chromosoma 41 (1973) 129
8. Flintoff W.F., Livingston E., Duff C., and Worton R.G. Moderate-level gene amplification in methotrexate-resistant Chinese hamster ovary cells is accompanied by chromosomal translocations at or near the site of the amplified DHFR gene. Mol. Cell. Biol. 4 (1984) 69
9. Gorman C., and Bullock C. Site-specific gene targeting for gene expression in eukaryotes. Curr. Opin. Biotechnol. 11 (2000) 455
10. Hirata R., Chamberlain J., Dong R., and Russell D.W. Targeted transgene insertion into human chromosomes by adeno-associated virus vectors. Nat. Biotechnol. 20 (2002) 735
11. Julian D., and Mitchell R. Chromosome localization and gene-copy-number quantification of three random integrations in Chinese hamster ovary cells and their amplified cell lines using fluorescence in situ hybridization. Biotechnol. Appl. Biochem. 33 (2001) 99
12. Kim N.S., Kim S.J., and Lee G.M. Clonal variability within dihydrofolate reductase-mediated gene amplified Chinese hamster ovary cells: stability in the absence of selective pressure. Biotechnol. Bioeng. 60 (1998) 679
13. Kim S.J., Kim N.S., Ryu C.J., Hong H.J., and Lee G.M. Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure. Biotechnol. Bioeng. 58 (1998) 73
14. Kito M., Itami S., Fukano Y., Yamana K., and Shibui T. Construction of engineered CHO strains for high-level production of recombinant proteins. Appl. Microbiol. Biotechnol. 60 (2002) 442
15. Knight D.M., Trinh H., Le J., Siegel S., Shealy D., McDonough M., Scallon B., Moore M.A., Vilcek J., Daddona P., and Ghrayeb J. Construction and initial characterization of a mouse-human chimeric anti-TNF-antibody. Mol. Immunol. 30 (1993) 1443
16. Koch K.S., Aoki T., Wang Y., Atkinson A.E., Gleiberman A.S., Glebov K., and Leffert H.L. Site-specific integration of targeted DNA into animal cell genomes. Gene 249 (2000) 135
17. Koetsier P.A., Schorr J., and Doerfler W. A rapid optimized protocol for downward alkaline southern blotting of DNA. BioTechniques 15 (1993) 260
18. Maniatis T., Fritsch E.F., and Sambrook J. Molecular Cloning: A Laboratory Manual. second ed. (1989), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
19. Meulien P., Faure T., Mischler F., Harrer H., Ulrich P., Bouderbala B., Dott K., Sainte M.M., Mazaurier C., and Wiesel M.L. A new recombinant procoagulant protein derived from the cDNA encoding human factor VIII. Protein Eng. 2 (1988) 301
20. Page M.J., and Sydenham M.A. High level expression of the humanized monoclonal antibody CAMPATH-1H in Chinese hamster ovary cells. Biotechnology 9 (1991) 64
21. Phi-Van L., and Stratling W.H. Dissection of the ability of the chicken lysozyme gene 5′ matrix attachment region to stimulate transgene expression and to dampen position effects. Biochemistry 35 (1996) 10735
22. Ray M., and Mohandas T. Proposed nomenclature for the Chinese hamster chromosomes (Cricetulus griseus). In: Hamerton J.L. (Ed). Report of the Committee on Chromosome Markers. Cytogenet. Cell Genet. 16 (1976) 83
23. Rolig R.L., Layher S.K., Santi B., Adair G.M., Gu F., Rainbow A.J., and Nairn R.S. Survival, mutagenesis, and host cell reactivation in a Chinese hamster ovary cell ERCC1 knock-out mutant. Mutagenesis 12 (1997) 277
24. Siciliano M.J., Stallings R.L., and Adair G.M. The genetic map of the Chinese hamster and the genetic consequences of chromosomal rearrangements in CHO cells. In: Gottesman M.M. (Ed). Molecular Cell Genetics (1985), Wiley, New York 95
25. Stein I., Itin A., Einat P., Skaliter R., Grossman Z., and Keshet E. Translation of vascular endothelial growth factor mRNA by internal ribosome entry: implications for translation under hypoxia. Mol. Cell. Biol. 18 (1998) 3112
26. Wang Y., DeMayo F.J., Tsai S.Y., and O'Malley B.W. Ligand-inducible and liver-specific target gene expression in transgenic mice. Nat. Biotechnol. 15 (1997) 239
27. Weidle U.H., Buckel P., and Wienberg J. Amplified expression constructs for human tissue-type plasminogen activator in Chinese hamster ovary cells: instability in the absence of selective pressure. Gene 66 (1988) 193
28. Wurm F.M., and Petropoulos C.J. Plasmid integration, amplification and cytogenetics in CHO cells: questions and comments. Biologicals 22 (1994) 95
29. Wurm F.M., Pallavicini M.G., and Arathoon R. Integration and stability of CHO amplicons containing plasmid sequences. Dev. Biol. Stand. 76 (1992) 69
30. Yoshikawa T., Nakanishi F., Ogura Y., Oi D., Omasa T., Katakura Y., Kishimoto M., and Suga K. Amplified gene location in chromosomal DNA affected recombinant protein production and stability of amplified genes. Biotechnol. Prog. 16 (2000) 710-715