Generation of a triple-gene knockout mammalian cell line using engineered zinc-finger nucleases

Biotechnology and Bioengineering

Volumn 106, Issue 1, 2010, Pages 97-105

  Liu, Pei Qi ^a   Chan, Edmond M ^a   Cost, Gregory J ^a   Zhang, Lin ^b   Wang, Jianbin ^a   Miller, Jeffrey C ^a   Guschin, Dmitry Y ^a   Reik, Andreas ^a   Holmes, Michael C ^a   Mott, John E ^b   Collingwood, Trevor N ^c   Gregory, Philip D ^a  

^a SANGAMO BIOSCIENCES INC   (United States)

^b PFIZER INC   (United States)

^c Sigma Aldrich Co   (United States)

Author keywords

Cell line engineering; DHFR; Fut8; GS; Knockout; Zinc finger nuclease

Indexed keywords

CELL LINE ENGINEERING; CELL LINES; CELL-BASED; DNA MUTATION; DRUG DISCOVERY; EXISTING METHOD; GENE DELETION; GENE KNOCKOUT; GENOME ENGINEERING; HOMOLOGOUS RECOMBINATION; MAMMALIAN CELL LINES; MAMMALIAN CELLS;

BINDING ENERGY; BINDING SITES; BIOCHEMISTRY; GENES; MAMMALS; ZINC;

CELL CULTURE;

GLUTAMATE AMMONIA LIGASE; NUCLEASE; ZINC FINGER PROTEIN;

ANIMAL CELL; ARTICLE; BINDING SITE; CHO CELL; CONTROLLED STUDY; GENE DISRUPTION; GENE FREQUENCY; GENE MUTATION; GENETIC ENGINEERING; KNOCKOUT GENE; MAMMAL CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; NULL ALLELE; PHENOTYPE;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; DEOXYRIBONUCLEASES; GENE KNOCKOUT TECHNIQUES; ZINC FINGERS;

MAMMALIA;

EID: 77951599072     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22654     Document Type: Article

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