BAK and BAX deletion using zinc-finger nucleases yields apoptosis-resistant CHO cells

Biotechnology and Bioengineering

Volumn 105, Issue 2, 2010, Pages 330-340

  Cost, Gregory J ^a   Freyvert, Yevgeniy ^a   Vafiadis, Annamaria ^b   Santiago, Yolanda ^a   Miller, Jeffrey C ^a   Rebar, Edward ^a   Collingwood, Trevor N ^a,c   Snowden, Andrew ^b   Gregory, Philip D ^a  

^a SANGAMO BIOSCIENCES INC   (United States)

^b GENENTECH INC   (United States)

^c Sigma Aldrich Co   (United States)

Author keywords

Biopharmaceutical; Cell death; Fermenter; Homologous recombination

Indexed keywords

APOPTOSIS; APOPTOTIC; APOPTOTIC PROTEINS; APOPTOTIC STIMULI; BIOPHARMACEUTICAL; CASPASES; CELL DENSITY; CHINESE HAMSTER OVARY CELLS; CHO CELL; GENE DISRUPTIONS; HOMOLOGOUS RECOMBINATION; IN-CELL; INDUSTRIAL PROTEINS; METABOLIC STRESS; NORMAL GROWTH; NUCLEASE CLEAVAGE; OVER-EXPRESSION; PERMEABILIZATION; SCALE-DOWN; SHAKE FLASKS; SODIUM BUTYRATE; STARVATION CONDITIONS; STAUROSPORINES; SUSPENSION CULTURES; VIABLE CELLS; WILD TYPES;

BIOELECTRIC POTENTIALS; BOTTLES; CELL DEATH; FERMENTERS; PLANTS (BOTANY); PROTEINS; SODIUM; ZINC;

CELL CULTURE;

CASPASE; IMMUNOGLOBULIN G; PROTEIN BAK; PROTEIN BAX; STAUROSPORINE; ZINC FINGER PROTEIN;

ANIMAL CELL CULTURE; APOPTOSIS; ARTICLE; BAK GENE; BAX GENE; BIOREACTOR; CELL DENSITY; CELL GROWTH; CHO CELL; CONTROLLED STUDY; DNA REPAIR; ENZYME ACTIVATION; GENE; GENE DELETION; GENE DISRUPTION; NONHUMAN; PROTEIN CLEAVAGE; PROTEIN SYNTHESIS; STARVATION; SUSPENSION CELL CULTURE; WILD TYPE;

ANIMALS; APOPTOSIS; BASE SEQUENCE; BCL-2-ASSOCIATED X PROTEIN; BIOTECHNOLOGY; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CRICETULUS; FEMALE; GENE DELETION; IMMUNOGLOBULIN G; MOLECULAR SEQUENCE DATA; OVARY; ZINC FINGERS;

CRICETULUS GRISEUS;

EID: 74849089122     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22541     Document Type: Article

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