Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector

Nucleic Acids Research

Volumn 42, Issue 19, 2014, Pages

  Kabadi, Ami M ^a   Ousterout, David G ^a   Hilton, Isaac B ^a   Gersbach, Charles A ^a,b,c  

^a Duke University   (United States)

^b DUKE UNIVERSITY   (United States)

^c DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CAS9 PROTEIN; CRISPR ASSOCIATED PROTEIN; DNA DIRECTED RNA POLYMERASE III; LENTIVIRUS VECTOR; REGULATOR PROTEIN; UNCLASSIFIED DRUG; DNA BINDING PROTEIN; GUIDE RNA; TRANSACTIVATOR PROTEIN;

ARTICLE; CAS9 GENE; CELL IMMORTALIZATION; CONTROLLED STUDY; DNA MODIFICATION; GENE ACTIVATION; GENE EXPRESSION; GENE INDUCTION; GENETIC ENGINEERING; HUMAN; HUMAN CELL; LENTIVIRINAE; MOLECULAR CLONING; NONHUMAN; PRIMARY CELL CULTURE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; REPORTER GENE; SKIN FIBROBLAST; TRANSCRIPTION REGULATION; CELL CULTURE; CELL ENGINEERING; CRISPR CAS SYSTEM; FIBROBLAST; GENE VECTOR; GENETICS; GENOME; HEK293 CELL LINE; METABOLISM; TRANSCRIPTION INITIATION;

CELL ENGINEERING; CELLS, CULTURED; CRISPR-ASSOCIATED PROTEINS; CRISPR-CAS SYSTEMS; DNA-BINDING PROTEINS; FIBROBLASTS; GENETIC VECTORS; GENOME; HEK293 CELLS; HUMANS; LENTIVIRUS; RNA, GUIDE; TRANS-ACTIVATORS; TRANSCRIPTIONAL ACTIVATION;

EID: 84922535144     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gku749     Document Type: Article

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