High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells

Nature

Volumn 509, Issue 7501, 2014, Pages 487-491

  Zhou, Yuexin ^a   Zhu, Shiyou ^a   Cai, Changzu ^a   Yuan, Pengfei ^a   Li, Chunmei ^a   Huang, Yanyi ^a   Wei, Wensheng ^a  

^a PEKING UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ANTHRAX TOXIN; DIPHTHERIA TOXIN;

BACTERIUM; GENE EXPRESSION; GENETIC ENGINEERING; GENOMICS; TOXICITY; TOXIN;

ARTICLE; CONTROLLED STUDY; CRISPR CAS SYSTEM; ESSENTIAL GENE; FUNCTIONAL GENOMICS; GENE IDENTIFICATION; GENETIC SCREENING; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL;

ANTIGENS, BACTERIAL; BACTERIAL TOXINS; CELL LINE; CELLS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-ASSOCIATED PROTEINS; DIPHTHERIA TOXIN; GENE LIBRARY; GENOMICS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; INDEL MUTATION; LENTIVIRUS; ORGANIC CATION TRANSPORTER 1; REPRODUCIBILITY OF RESULTS; RNA, GUIDE;

EID: 84900861730     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature13166     Document Type: Article

References (26)

1. Porteus, M. H. & Baltimore, D. Chimeric nucleases stimulate gene targeting in human cells. Science 300, 763 (2003).
2. Miller, J. C. et al. An improved zinc-finger nuclease architecture for highly specific genome editing. Nature Biotechnol. 25, 778-785 (2007).
3. Wood, A. J. et al. Targeted genome editing across species using ZFNs and TALENs. Science 333, 307 (2011).
4. Miller, J. C. et al. A TALE nuclease architecture for efficient genome editing. Nature Biotechnol. 29, 143-148 (2011).
5. Zhang, F. et al. Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nature Biotechnol. 29, 149-153 (2011).
6. Cong, L. et al. Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819-823 (2013).
7. Mali, P. et al. RNA-guided human genome engineering via Cas9. Science 339, 823-826 (2013).
8. Wang, T., Wei, J. J., Sabatini, D. M. & Lander, E. S. Genetic screens in human cells using the CRISPR-Cas9 system. Science 343, 80-84 (2014).
9. Shalem, O. et al. Genome-scale CRISPR-Cas9 knockout screening in human cells. Science 343, 84-87 (2014).
10. Barrangou, R. et al. CRISPR provides acquired resistance against viruses in prokaryotes. Science 315, 1709-1712 (2007).
11. Jinek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816-821 (2012).
12. Lin, B. R. & Natarajan, V.Negative regulation of humanU6snRNApromoter byp38 kinase through Oct-1. Gene 497, 200-207 (2012).
13. Engler, C., Gruetzner, R., Kandzia, R. & Marillonnet, S. Golden gate shuffling: a onepot DNA shuffling method based on type IIs restriction enzymes. PLoS ONE 4, e5553 (2009).
14. Mussolino, C. et al. A novel TALE nuclease scaffold enables high genome editing activity in combination with lowtoxicity.Nucleic AcidsRes.39, 9283-9293(2011).
15. Anders, S. & Huber, W. Differential expression analysis for sequence count data. Genome Biol. 11, R106 (2010).
16. Bradley, K. A., Mogridge, J., Mourez, M., Collier, R. J. & Young, J. A. Identification of the cellular receptor for anthrax toxin. Nature 414, 225-229 (2001).
17. Naglich, J. G., Metherall, J. E., Russell, D. W. & Eidels, L. Expression cloning of a diphtheria toxin receptor: identity with a heparin-binding EGF-like growth factor precursor. Cell 69, 1051-1061 (1992).
18. Yang, J. et al. ULtiMATE system for rapid assembly of customized TAL effectors. PLoS ONE 8, e75649 (2013).
19. Scobie, H. M., Rainey, G. J., Bradley, K. A. & Young, J. A. Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor. Proc. Natl Acad. Sci. USA 100, 5170-5174 (2003).
20. Liu, S. et al. Capillary morphogenesis protein-2 is the major receptor mediating lethality of anthrax toxin in vivo. Proc. Natl Acad. Sci. USA 106, 12424-12429 (2009).
21. Zuber, J. et al. Toolkit for evaluating genes required for proliferation and survival using tetracycline-regulated RNAi. Nature Biotechnol. 29, 79-83 (2011).
22. Gibson, D. G. et al. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nature Methods 6, 343-345 (2009).
23. Gibson, D. G. Enzymatic assembly of overlapping DNA fragments. Methods Enzymol. 498, 349-361 (2011).
24. Wei, W., Lu, Q., Chaudry, G. J., Leppla, S. H. & Cohen, S. N. The LDL receptor-related protein LRP6 mediates internalization and lethality of anthrax toxin. Cell 124, 1141-1154 (2006).
25. Kim, J. H. et al. High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice. PLoS ONE 6, e18556 (2011).
26. Chang, N. et al. Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos. Cell Res. 23, 465-472 (2013).