One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment

Biotechnology Journal

Volumn 10, Issue 9, 2015, Pages 1446-1456

  Grav, Lise Marie ^a   Lee, Jae Seong ^a   Gerling, Signe ^a   Kallehauge, Thomas Beuchert ^a   Hansen, Anders Holmgaard ^a   Kol, Stefan ^a   Lee, Gyun Min ^b   Pedersen, Lasse Ebdrup ^a   Kildegaard, Helene Faustrup ^a  

^a Chalmers University of Technology   (Denmark)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Chinese hamster ovary cells; CRISPR Cas9; Deep sequencing; Genome editing; Multiplexing

Indexed keywords

CELL CULTURE; CELL DEATH; CELLS; CLONE CELLS; CLONING; CYTOLOGY; GENES; LAKES; MULTIPLEXING;

CHINESE HAMSTER OVARY CELLS; CRISPR/CAS9; DEEP SEQUENCING; EDITING TECHNOLOGY; GENE DISRUPTIONS; GENOME ENGINEERING; TARGET PREDICTION; TRANSFECTED CELLS;

CELL ENGINEERING;

CRISPR ASSOCIATED PROTEIN; ENDONUCLEASE; ENDONUCLEASE CAS9; GREEN FLUORESCENT PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; APOPTOSIS; ARTICLE; BAK GENE; BAX GENE; CHO CELL LINE; CONTROLLED STUDY; CRISPR CAS SYSTEM; ENRICHMENT CULTURE; FEMALE; FUCOSYLATION; FUT8 GENE; GENE; GENE DISRUPTION; GENE FREQUENCY; GENE INACTIVATION; GENE LOCUS; GENETIC ENGINEERING AND GENE TECHNOLOGY; GENETIC RESISTANCE; GENOME; GENOME EDITING; INDEL MUTATION; MOLECULAR CLONING; NONHUMAN; PREDICTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; TRANSIENT EXPRESSION; WILD TYPE; ANIMAL; BIOTECHNOLOGY; CELL SURVIVAL; CRICETULUS; FLOW CYTOMETRY; GENETICS; HAMSTER; METABOLISM; PROCEDURES; RNA EDITING;

ANIMALS; APOPTOSIS; BIOTECHNOLOGY; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; CRISPR-CAS SYSTEMS; FLOW CYTOMETRY; GENE KNOCKOUT TECHNIQUES; GREEN FLUORESCENT PROTEINS; RNA EDITING;

EID: 84940601641     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201500027     Document Type: Article

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