Polishing the craft of genetic diversity creation in directed evolution

Biotechnology Advances

Volumn 31, Issue 8, 2013, Pages 1707-1721

  Tee, Kang Lan ^a   Wong, Tuck Seng ^b  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Directed evolution; DNA recombination; Genetic diversity; Genome engineering; Metabolic engineering; Random mutagenesis; Site directed mutagenesis; Synthetic biology

Indexed keywords

DIRECTED EVOLUTION; DNA RECOMBINATION; GENETIC DIVERSITY; GENOME ENGINEERING; RANDOM MUTAGENESIS; SITE DIRECTED MUTAGENESIS; SYNTHETIC BIOLOGY;

CLONING; GENES; METABOLIC ENGINEERING; MUTAGENESIS; POLYMERASE CHAIN REACTION;

BIODIVERSITY;

BIOTECHNOLOGY; EVOLUTION; GENETIC ENGINEERING; GENOME; PROTEIN; RECOMBINATION;

BP; C REACTIVE PROTEIN; DEOXYRIBONUCLEOTIDE TRIPHOSPHATE; DNA; KLENOW FRAGMENT; MESYLIC ACID ETHYL ESTER; NUCLEOTIDE; PARATHION; PHOSPHOROTHIOIC ACID; TIM; TOSYLARGININE METHYL ESTER; ZINC FINGER NUCLEASE;

2′-DEOXY-P-NUCLEOSIDE-5′-TRIPHOSPHATE; 2′-DEOXYINOSINE 5′-TRIPHOSPHATE; 7-DEAZA-7-(TRIETHYLSILYLETHYNYL)DEOXYADENOSINE TRIPHOSPHATE; 8-OXO-2′-DEOXYGUANOSINE-5′-TRIPHOSPHATE; 8-OXO-DGTP; AAV; ABI-REC; ADENO-ASSOCIATED VIRUS; ANIMAL; ASYMMETRIC BRIDGE PCR WITH INTRAMOLECULAR HOMOLOGOUS RECOMBINATION; BASE PAIRS; BIOENGINEERING; CAMP RECEPTOR PROTEIN; CAST; CIRCULAR POLYMERASE EXTENSION CLONING; COMBINATORIAL ACTIVE-SITE SATURATION TEST; CPEC; DA(TESE)TP; DGRS; DIRECTED EVOLUTION; DIRECTED MOLECULAR EVOLUTION; DITP; DIVERSITY-GENERATING RETROELEMENTS; DNA RECOMBINATION; DOUBLE-STRANDED DNA; DPTP; DSDNA; DUAL APPROACH TO RANDOM CHEMICAL MUTAGENESIS; DUARCHEM; EMP; EMS; ENOYL ACP REDUCTASE; ENOYL-ACYL CARRIER PROTEIN REDUCTASE; EPPCR; EPRCA; ERROR-PRONE POLYMERASE CHAIN REACTION; ERROR-PRONE ROLLING CIRCLE AMPLIFICATION; EXPONENTIAL MEGAPRIMING PCR; GENE LIBRARY; GENE OF INTEREST; GENETIC VARIABILITY; GENOME ENGINEERING; GLUTATHIONE-S-TRANSFERASE; GOI; GST; HUMAN; INCREMENTAL TRUNCATION FOR THE CREATION OF HYBRID ENZYMES; INDEL MUTATION; ITCHY; KF; MAP; MEGAPRIMED AND LIGASE-FREE PCR-BASED METHOD FOR SITE-DIRECTED MUTAGENESIS; MEGAPRIMER PCR OF WHOLE PLASMID; MEGAWHOP; METABOLIC ENGINEERING; MGS; MLF-SDM; MOLECULAR CLONING; MOUSE; MUTAGENESIS ASSISTANT PROGRAM; MUTATION GENERATION SYSTEM; NEB; NEW ENGLAND BIOLABS; NICKING DNA ENDONUCLEASE; NIDE; NON-HOMOLOGOUS RANDOM RECOMBINATION; NRR; NT; OLTA; ONE-POT SIMPLE METHODOLOGY FOR CASETTE RANDOMIZATION AND RECOMBINATION; OSCARR; OVERLAP EXTENSION PCR AND TA CLONING; P-BENZOYLPHENYLALANINE; PBPA; PCR; PCR PRODUCTION OF CIRCULAR PLASMID; PERMUTATION USING TRANSPOSASE ENGINEERING; PERMUTE; PFLF-MSDM; PHOSPHOROTHIOATE-BASED DNA RECOMBINATION; PHOSPHOROTHIOATE-BASED LIGASE-INDEPENDENT GENE CLONING; PHOSPHORYLATION-FREE AND LIGASE-FREE PCR-BASED METHOD FOR MULTIPLE SDM; PLICING; POLYMERASE CHAIN REACTION; PPCP; PS; PTREC; RCA; RESTRICTION ENZYMES; RESTRICTION-FREE CLONING; RETICULOENDOTHELIAL SYSTEM; REVIEW; RF CLONING; RGEN; RNA-GUIDED ENDONUCLEASE; ROLLING CIRCLE AMPLIFICATION; SATURATION MUTAGENESIS; SDM; SEAMLESS ENZYME-FREE CLONING; SEAMLESS LIGATION CLONING EXTRACT; SEFC; SEQUENCE HOMOLOGY-INDEPENDENT PROTEIN RECOMBINATION; SEQUENCE SATURATION MUTAGENESIS; SESAM; SHIPREC; SINGLE-PRIMER REACTIONS IN PARALLEL; SINGLE-STRANDED DNA; SINGLE-TUBE RESTRICTION-BASED ULTRAFILTRATION CLONING; SITE DIRECTED MUTAGENESIS; SITE-DIRECTED MUTAGENESIS; SLICE; SPRINP; SSDNA; STAGGERED EXTENSION PROCESS; STEP; STRU-CLONING; SYNTHETIC BIOLOGY; T(S); T(V); TAGTEAM; TALENS; TANDEM REPEAT INSERTION; TARGETING GLYCOSYLASES TO EMBEDDED ARRAYS FOR MUTAGENESIS; TMGS-PCR; TPCR; TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASES; TRANSCRIPTION-ASSOCIATED MUTATION; TRANSFER-PCR; TRANSITIONS; TRANSPOSON INTEGRATION MEDIATED MUTAGENESIS; TRANSVERSIONS; TRINEX; TRINS; TRINUCLEOTIDE EXCHANGE; TRUNCATED METAGENOMIC GENE-SPECIFIC PCR; UDG; URACIL-DNA GLYCOSYLASE; USER FRIENDLY DNA RECOMBINATION; USEREC; ZFN;

2′-DEOXY-P-NUCLEOSIDE-5′-TRIPHOSPHATE; 2′-DEOXYINOSINE 5′-TRIPHOSPHATE; 7-DEAZA-7-(TRIETHYLSILYLETHYNYL)DEOXYADENOSINE TRIPHOSPHATE; 8-OXO-2′-DEOXYGUANOSINE-5′-TRIPHOSPHATE; 8-OXO-DGTP; AAV; ABI-REC; ADENO-ASSOCIATED VIRUS; ASYMMETRIC BRIDGE PCR WITH INTRAMOLECULAR HOMOLOGOUS RECOMBINATION; BASE PAIRS; BP; CAMP RECEPTOR PROTEIN; CAST; CIRCULAR POLYMERASE EXTENSION CLONING; COMBINATORIAL ACTIVE-SITE SATURATION TEST; CPEC; CRP; DA(TESE)TP; DEOXYRIBONUCLEIC ACID; DEOXYRIBONUCLEOTIDE TRIPHOSPHATE; DGRS; DIRECTED EVOLUTION; DITP; DIVERSITY-GENERATING RETROELEMENTS; DNA; DNA RECOMBINATION; DNTP; DOUBLE-STRANDED DNA; DPTP; DSDNA; DUAL APPROACH TO RANDOM CHEMICAL MUTAGENESIS; DUARCHEM; EMP; EMS; ENOYL ACP REDUCTASE; ENOYL-ACYL CARRIER PROTEIN REDUCTASE; EPPCR; EPRCA; ERROR-PRONE POLYMERASE CHAIN REACTION; ERROR-PRONE ROLLING CIRCLE AMPLIFICATION; ETHYL METHANE SULFONATE; EXPONENTIAL MEGAPRIMING PCR; GENE OF INTEREST; GENETIC DIVERSITY; GENOME ENGINEERING; GLUTATHIONE-S-TRANSFERASE; GOI; GST; INCREMENTAL TRUNCATION FOR THE CREATION OF HYBRID ENZYMES; INDEL; INSERTION AND DELETION; ITCHY; KF; KLENOW FRAGMENT; MAP; MEGAPRIMED AND LIGASE-FREE PCR-BASED METHOD FOR SITE-DIRECTED MUTAGENESIS; MEGAPRIMER PCR OF WHOLE PLASMID; MEGAWHOP; METABOLIC ENGINEERING; MGS; MLF-SDM; MUTAGENESIS ASSISTANT PROGRAM; MUTATION GENERATION SYSTEM; NEB; NEW ENGLAND BIOLABS; NICKING DNA ENDONUCLEASE; NIDE; NON-HOMOLOGOUS RANDOM RECOMBINATION; NRR; NT; NUCLEOTIDE; OLTA; ONE-POT SIMPLE METHODOLOGY FOR CASETTE RANDOMIZATION AND RECOMBINATION; OSCARR; OVERLAP EXTENSION PCR AND TA CLONING; P-BENZOYLPHENYLALANINE; PBPA; PCR; PCR PRODUCTION OF CIRCULAR PLASMID; PERMUTATION USING TRANSPOSASE ENGINEERING; PERMUTE; PFLF-MSDM; PHOSPHOROTHIOATE; PHOSPHOROTHIOATE-BASED DNA RECOMBINATION; PHOSPHOROTHIOATE-BASED LIGASE-INDEPENDENT GENE CLONING; PHOSPHORYLATION-FREE AND LIGASE-FREE PCR-BASED METHOD FOR MULTIPLE SDM; PLICING; POLYMERASE CHAIN REACTION; PPCP; PS; PTREC; RANDOM MUTAGENESIS; RCA; RES; RESTRICTION ENZYMES; RESTRICTION-FREE CLONING; RF CLONING; RGEN; RNA-GUIDED ENDONUCLEASE; ROLLING CIRCLE AMPLIFICATION; SDM; SEAMLESS ENZYME-FREE CLONING; SEAMLESS LIGATION CLONING EXTRACT; SEFC; SEQUENCE HOMOLOGY-INDEPENDENT PROTEIN RECOMBINATION; SEQUENCE SATURATION MUTAGENESIS; SESAM; SHIPREC; SINGLE-PRIMER REACTIONS IN PARALLEL; SINGLE-STRANDED DNA; SINGLE-TUBE RESTRICTION-BASED ULTRAFILTRATION CLONING; SITE-DIRECTED MUTAGENESIS; SLICE; SPRINP; SSDNA; STAGGERED EXTENSION PROCESS; STEP; STRU-CLONING; SYNTHETIC BIOLOGY; T(S); T(V); TAGTEAM; TALENS; TAM; TANDEM REPEAT INSERTION; TARGETING GLYCOSYLASES TO EMBEDDED ARRAYS FOR MUTAGENESIS; TIM; TMGS-PCR; TPCR; TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASES; TRANSCRIPTION-ASSOCIATED MUTATION; TRANSFER-PCR; TRANSITIONS; TRANSPOSON INTEGRATION MEDIATED MUTAGENESIS; TRANSVERSIONS; TRINEX; TRINS; TRINUCLEOTIDE EXCHANGE; TRUNCATED METAGENOMIC GENE-SPECIFIC PCR; UDG; URACIL-DNA GLYCOSYLASE; USER FRIENDLY DNA RECOMBINATION; USEREC; ZFN; ZINC FINGER NUCLEASE;

ANIMALS; BIOENGINEERING; CLONING, MOLECULAR; DIRECTED MOLECULAR EVOLUTION; GENE LIBRARY; GENETIC VARIATION; HUMANS; MICE; MUTAGENESIS, SITE-DIRECTED;

EID: 84887993076     PISSN: 07349750     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biotechadv.2013.08.021     Document Type: Review

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