-
1
-
-
34247173915
-
Thermostabilization of Pichia stipitis xylitol dehydrogenase by mutation of structural zinc-binding loop
-
Annaluru N., Watanabe S., Pack S.P., Saleh A.A., Kodaki T., Makino K. Thermostabilization of Pichia stipitis xylitol dehydrogenase by mutation of structural zinc-binding loop. J. Biotechnol. 2007, 129:717-722.
-
(2007)
J. Biotechnol.
, vol.129
, pp. 717-722
-
-
Annaluru, N.1
Watanabe, S.2
Pack, S.P.3
Saleh, A.A.4
Kodaki, T.5
Makino, K.6
-
2
-
-
33846398126
-
Thermostable red and green light-producing firefly luciferase mutants for bioluminescent reporter applications
-
Branchini B.R., Ablamsky D.M., Murtiashaw M.H., Uzasci L., Fraga H., Southworth T.L. Thermostable red and green light-producing firefly luciferase mutants for bioluminescent reporter applications. Anal. Biochem. 2007, 361:253-262.
-
(2007)
Anal. Biochem.
, vol.361
, pp. 253-262
-
-
Branchini, B.R.1
Ablamsky, D.M.2
Murtiashaw, M.H.3
Uzasci, L.4
Fraga, H.5
Southworth, T.L.6
-
3
-
-
33744924721
-
Mutations on aromatic residues of the active site to alter selectivity of the Sulfolobus solfataricus maltooligosyltrehalose synthase
-
Fang T.Y., Tseng W.C., Chung Y.T., Pan C.H. Mutations on aromatic residues of the active site to alter selectivity of the Sulfolobus solfataricus maltooligosyltrehalose synthase. J. Agric. Food Chem. 2006, 54:3585-3590.
-
(2006)
J. Agric. Food Chem.
, vol.54
, pp. 3585-3590
-
-
Fang, T.Y.1
Tseng, W.C.2
Chung, Y.T.3
Pan, C.H.4
-
4
-
-
34447645125
-
Protein engineering of Sulfolobus solfataricus maltooligosyltrehalose synthase to alter its selectivity
-
Fang T.Y., Tseng W.C., Pan C.H., Chun Y.T., Wang M.Y. Protein engineering of Sulfolobus solfataricus maltooligosyltrehalose synthase to alter its selectivity. J. Agric. Food Chem. 2007, 55:5588-5594.
-
(2007)
J. Agric. Food Chem.
, vol.55
, pp. 5588-5594
-
-
Fang, T.Y.1
Tseng, W.C.2
Pan, C.H.3
Chun, Y.T.4
Wang, M.Y.5
-
5
-
-
43049141115
-
Effects of introducing negative charges into the molecular surface of thermolysin by site-directed mutagenesis on its activity and stability
-
Takita T., Aono T., Sakurama H., Itoh T., Wada T., Minoda M., Yasukawa K., Inouye K. Effects of introducing negative charges into the molecular surface of thermolysin by site-directed mutagenesis on its activity and stability. Biochim. Biophys. Acta 2008, 1784:481-488.
-
(2008)
Biochim. Biophys. Acta
, vol.1784
, pp. 481-488
-
-
Takita, T.1
Aono, T.2
Sakurama, H.3
Itoh, T.4
Wada, T.5
Minoda, M.6
Yasukawa, K.7
Inouye, K.8
-
6
-
-
34548227042
-
Concurrent mutations in six amino acids in β-glucuronidase improve its thermostability
-
Xiong A.S., Peng R.H., Cheng Z.M., Li Y., Liu J.G., Zhuang J., Gao F., Xu F., Qiao Y.S., Zhang Z., Chen J.M., Yao Q.H. Concurrent mutations in six amino acids in β-glucuronidase improve its thermostability. Protein Eng. Des. Sel. 2007, 20:319-325.
-
(2007)
Protein Eng. Des. Sel.
, vol.20
, pp. 319-325
-
-
Xiong, A.S.1
Peng, R.H.2
Cheng, Z.M.3
Li, Y.4
Liu, J.G.5
Zhuang, J.6
Gao, F.7
Xu, F.8
Qiao, Y.S.9
Zhang, Z.10
Chen, J.M.11
Yao, Q.H.12
-
7
-
-
37249041070
-
Cumulative improvements of thermostability and pH-activity profile of Aspergillus niger PhyA phytase by site-directed mutagenesis
-
Zhang W., Lei X.G. Cumulative improvements of thermostability and pH-activity profile of Aspergillus niger PhyA phytase by site-directed mutagenesis. Appl. Microbiol. Biotechnol. 2008, 77:1033-1040.
-
(2008)
Appl. Microbiol. Biotechnol.
, vol.77
, pp. 1033-1040
-
-
Zhang, W.1
Lei, X.G.2
-
8
-
-
0031574019
-
Approaches to DNA mutagenesis: an overview
-
Ling M.M., Robinson B.H. Approaches to DNA mutagenesis: an overview. Anal. Biochem. 1997, 254:157-178.
-
(1997)
Anal. Biochem.
, vol.254
, pp. 157-178
-
-
Ling, M.M.1
Robinson, B.H.2
-
9
-
-
1842868489
-
PCR approaches to DNA mutagenesis and recombination: an overview
-
Shen B. PCR approaches to DNA mutagenesis and recombination: an overview. Methods Mol. Biol. 2002, 192:167-174.
-
(2002)
Methods Mol. Biol.
, vol.192
, pp. 167-174
-
-
Shen, B.1
-
10
-
-
27544507435
-
A rapid and efficient method for multiple-site mutagenesis with a modified overlap extension PCR
-
An Y., Ji J., Wu W., Lv A., Huang R., Wei Y. A rapid and efficient method for multiple-site mutagenesis with a modified overlap extension PCR. Appl. Microbiol. Biotechnol. 2005, 68:774-778.
-
(2005)
Appl. Microbiol. Biotechnol.
, vol.68
, pp. 774-778
-
-
An, Y.1
Ji, J.2
Wu, W.3
Lv, A.4
Huang, R.5
Wei, Y.6
-
11
-
-
0037098946
-
Site-directed mutagenesis by the megaprimer PCR method: variations on a theme for simultaneous introduction of multiple mutations
-
Angelaccio S., Bonaccorsi di Patti M.C. Site-directed mutagenesis by the megaprimer PCR method: variations on a theme for simultaneous introduction of multiple mutations. Anal. Biochem. 2002, 306:346-349.
-
(2002)
Anal. Biochem.
, vol.306
, pp. 346-349
-
-
Angelaccio, S.1
Bonaccorsi di Patti, M.C.2
-
12
-
-
0036841628
-
Creating randomized amino acid libraries with the QuikChange Multi Site-Directed Mutagenesis Kit
-
Hogrefe H.H., Cline J., Youngblood G.L., Allen R.M. Creating randomized amino acid libraries with the QuikChange Multi Site-Directed Mutagenesis Kit. Biotechniques 2002, 33:1158-1165.
-
(2002)
Biotechniques
, vol.33
, pp. 1158-1165
-
-
Hogrefe, H.H.1
Cline, J.2
Youngblood, G.L.3
Allen, R.M.4
-
13
-
-
42649089776
-
Single-step overlap-primer-walk polymerase chain reaction for multiple mutagenesis without overlap extension
-
Kumar R., Rajagopal K. Single-step overlap-primer-walk polymerase chain reaction for multiple mutagenesis without overlap extension. Anal. Biochem. 2008, 377:105-107.
-
(2008)
Anal. Biochem.
, vol.377
, pp. 105-107
-
-
Kumar, R.1
Rajagopal, K.2
-
14
-
-
0346362261
-
Multiple site-directed mutagenesis of more than 10 sites simultaneously and in a single round
-
Seyfang A., Jin J.H. Multiple site-directed mutagenesis of more than 10 sites simultaneously and in a single round. Anal. Biochem. 2004, 324:285-291.
-
(2004)
Anal. Biochem.
, vol.324
, pp. 285-291
-
-
Seyfang, A.1
Jin, J.H.2
-
15
-
-
40649107834
-
A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method
-
Tseng W.C., Lin J.W., Wei T.Y., Fang T.Y. A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method. Anal. Biochem. 2008, 375:376-378.
-
(2008)
Anal. Biochem.
, vol.375
, pp. 376-378
-
-
Tseng, W.C.1
Lin, J.W.2
Wei, T.Y.3
Fang, T.Y.4
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