-
1
-
-
23444450226
-
Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
-
Chica RA, Doucet N, Pelletier JN, (2005) Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design. Curr Opin Biotechnol 16: 378-384.
-
(2005)
Curr Opin Biotechnol
, vol.16
, pp. 378-384
-
-
Chica, R.A.1
Doucet, N.2
Pelletier, J.N.3
-
2
-
-
35148850038
-
Steering directed protein evolution: strategies to manage combinatorial complexity of mutant libraries
-
Wong TS, Roccatano D, Schwaneberg U, (2007) Steering directed protein evolution: strategies to manage combinatorial complexity of mutant libraries. Environ Microbiol 9: 2645-2659.
-
(2007)
Environ Microbiol
, vol.9
, pp. 2645-2659
-
-
Wong, T.S.1
Roccatano, D.2
Schwaneberg, U.3
-
3
-
-
65249152992
-
Advances in generating functional diversity for directed protein evolution
-
Shivange AV, Marienhagen J, Mundhada H, Schenk A, Schwaneberg U, (2009) Advances in generating functional diversity for directed protein evolution. Curr Opin Chem Biol 13: 19-25.
-
(2009)
Curr Opin Chem Biol
, vol.13
, pp. 19-25
-
-
Shivange, A.V.1
Marienhagen, J.2
Mundhada, H.3
Schenk, A.4
Schwaneberg, U.5
-
4
-
-
78049263384
-
Improving biocatalyst performance by integrating statistical methods into protein engineering
-
Brouk M, Nov Y, Fishman A, (2010) Improving biocatalyst performance by integrating statistical methods into protein engineering. Appl Environ Microbiol 76: 6397-6403.
-
(2010)
Appl Environ Microbiol
, vol.76
, pp. 6397-6403
-
-
Brouk, M.1
Nov, Y.2
Fishman, A.3
-
5
-
-
77954274719
-
Iterative saturation mutagenesis accelerates laboratory evolution of enzyme stereoselectivity: rigorous comparison with traditional methods
-
Reetz MT, Prasad S, Carballeira JD, Gumulya Y, Bocola M, (2010) Iterative saturation mutagenesis accelerates laboratory evolution of enzyme stereoselectivity: rigorous comparison with traditional methods. J Am Chem Soc 132: 9144-9152.
-
(2010)
J Am Chem Soc
, vol.132
, pp. 9144-9152
-
-
Reetz, M.T.1
Prasad, S.2
Carballeira, J.D.3
Gumulya, Y.4
Bocola, M.5
-
6
-
-
0032052278
-
An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes
-
Kirsch RD, Joly E, (1998) An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes. Nucleic Acids Res 26: 1848-1850.
-
(1998)
Nucleic Acids Res
, vol.26
, pp. 1848-1850
-
-
Kirsch, R.D.1
Joly, E.2
-
7
-
-
33744475011
-
Directed evolution of enantioselective enzymes: iterative cycles of CASTing for probing protein-sequence space
-
Reetz MT, Wang LW, Bocola M, (2006) Directed evolution of enantioselective enzymes: iterative cycles of CASTing for probing protein-sequence space. Angew Chem, Int Ed Engl 45: 1236-1241.
-
(2006)
Angew Chem, Int Ed Engl
, vol.45
, pp. 1236-1241
-
-
Reetz, M.T.1
Wang, L.W.2
Bocola, M.3
-
8
-
-
0027314904
-
Mutagenic oligonucleotide-directed PCR amplification (Mod-PCR): an efficient method for generating random base substitution mutations in a DNA sequence element
-
Chiang LW, Kovari I, Howe MM, (1993) Mutagenic oligonucleotide-directed PCR amplification (Mod-PCR): an efficient method for generating random base substitution mutations in a DNA sequence element. PCR Methods Appl 2: 210-217.
-
(1993)
PCR Methods Appl
, vol.2
, pp. 210-217
-
-
Chiang, L.W.1
Kovari, I.2
Howe, M.M.3
-
9
-
-
0024520745
-
Site-directed mutagenesis by overlap extension using the polymerase chain reaction
-
Ho SN, Hunt HD, Horton RM, Pullen JK, Pease LR, (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77: 51-59.
-
(1989)
Gene
, vol.77
, pp. 51-59
-
-
Ho, S.N.1
Hunt, H.D.2
Horton, R.M.3
Pullen, J.K.4
Pease, L.R.5
-
10
-
-
0028290287
-
Codon cassette mutagenesis: a general method to insert or replace individual codons by using universal mutagenic cassettes
-
Kegler-Ebo DM, Docktor CM, DiMaio D, (1994) Codon cassette mutagenesis: a general method to insert or replace individual codons by using universal mutagenic cassettes. Nucleic Acids Res 22: 1593-1599.
-
(1994)
Nucleic Acids Res
, vol.22
, pp. 1593-1599
-
-
Kegler-Ebo, D.M.1
Docktor, C.M.2
DiMaio, D.3
-
11
-
-
18044363663
-
Whole plasmid mutagenic PCR for directed protein evolution
-
Matsumura I, Rowe LA, (2005) Whole plasmid mutagenic PCR for directed protein evolution. Biomol Eng 22: 73-79.
-
(2005)
Biomol Eng
, vol.22
, pp. 73-79
-
-
Matsumura, I.1
Rowe, L.A.2
-
12
-
-
33750329048
-
A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis
-
Peng RH, Xiong AS, Yao QH, (2006) A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis. Appl Microbiol Biotechnol 73: 234-240.
-
(2006)
Appl Microbiol Biotechnol
, vol.73
, pp. 234-240
-
-
Peng, R.H.1
Xiong, A.S.2
Yao, Q.H.3
-
13
-
-
34248567845
-
Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes
-
Reetz MT, Carballeira JD, (2007) Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes. Nat Protoc 2: 891-903.
-
(2007)
Nat Protoc
, vol.2
, pp. 891-903
-
-
Reetz, M.T.1
Carballeira, J.D.2
-
14
-
-
1642414512
-
Directed Evolution Library Creation: Methods and Protocols
-
Totowa, Humana Press
-
Arnold FH, Georgiou G, (2003) Directed Evolution Library Creation: Methods and Protocols. Totowa Humana Press.
-
(2003)
-
-
Arnold, F.H.1
Georgiou, G.2
-
15
-
-
0346362261
-
Multiple site-directed mutagenesis of more than 10 sites simultaneously and in a single round
-
Seyfang A, Jin JH, (2004) Multiple site-directed mutagenesis of more than 10 sites simultaneously and in a single round. Anal Biochem 324: 285-291.
-
(2004)
Anal Biochem
, vol.324
, pp. 285-291
-
-
Seyfang, A.1
Jin, J.H.2
-
16
-
-
0030851627
-
The elimination of primer-dimer accumulation in PCR
-
Brownie J, Shawcross S, Theaker J, Whitcombe D, Ferrie R, et al. (1997) The elimination of primer-dimer accumulation in PCR. Nucleic Acids Res 25: 3235-3241.
-
(1997)
Nucleic Acids Res
, vol.25
, pp. 3235-3241
-
-
Brownie, J.1
Shawcross, S.2
Theaker, J.3
Whitcombe, D.4
Ferrie, R.5
-
17
-
-
77952246399
-
MPprimer: a program for reliable multiplex PCR primer design
-
Shen Z, Qu W, Wang W, Lu Y, Wu Y, et al. (2010) MPprimer: a program for reliable multiplex PCR primer design. BMC Bioinf 11: 143.
-
(2010)
BMC Bioinf
, vol.11
, pp. 143
-
-
Shen, Z.1
Qu, W.2
Wang, W.3
Lu, Y.4
Wu, Y.5
-
18
-
-
0036841628
-
Creating randomized amino acid libraries with the QuikChange Multi Site-Directed Mutagenesis Kit
-
Hogrefe HH, Cline J, Youngblood GL, Allen RM, (2002) Creating randomized amino acid libraries with the QuikChange Multi Site-Directed Mutagenesis Kit. Biotechniques 33: 1158-1160, 1162,.
-
(2002)
Biotechniques
, vol.33
-
-
Hogrefe, H.H.1
Cline, J.2
Youngblood, G.L.3
Allen, R.M.4
-
19
-
-
34548014497
-
Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): a versatile tool for generating targeted libraries
-
Herman A, Tawfik DS, (2007) Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): a versatile tool for generating targeted libraries. Protein Eng, Des Sel 20: 219-226.
-
(2007)
Protein Eng, Des Sel
, vol.20
, pp. 219-226
-
-
Herman, A.1
Tawfik, D.S.2
-
20
-
-
33750353815
-
Directed evolution by accumulating tailored mutations: thermostabilization of lactate oxidase with less trade-off with catalytic activity
-
Hamamatsu N, Nomiya Y, Aita T, Nakajima M, Husimi Y, et al. (2006) Directed evolution by accumulating tailored mutations: thermostabilization of lactate oxidase with less trade-off with catalytic activity. Protein Eng, Des Sel 19: 483-489.
-
(2006)
Protein Eng, Des Sel
, vol.19
, pp. 483-489
-
-
Hamamatsu, N.1
Nomiya, Y.2
Aita, T.3
Nakajima, M.4
Husimi, Y.5
-
21
-
-
77956234144
-
Natural diversity to guide focused directed evolution
-
Jochens H, Bornscheuer UT, (2010) Natural diversity to guide focused directed evolution. ChemBioChem 11: 1861-1866.
-
(2010)
ChemBioChem
, vol.11
, pp. 1861-1866
-
-
Jochens, H.1
Bornscheuer, U.T.2
-
22
-
-
2342535792
-
Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution
-
Wong TS, Tee KL, Hauer B, Schwaneberg U, (2004) Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution. Nucleic Acids Res 32: e26.
-
(2004)
Nucleic Acids Res
, vol.32
-
-
Wong, T.S.1
Tee, K.L.2
Hauer, B.3
Schwaneberg, U.4
-
23
-
-
0024508604
-
Phosphorothioates in molecular biology
-
Eckstein F, Gish G, (1989) Phosphorothioates in molecular biology. Trends Biochem Sci 14: 97-100.
-
(1989)
Trends Biochem Sci
, vol.14
, pp. 97-100
-
-
Eckstein, F.1
Gish, G.2
-
24
-
-
1842525539
-
Enhancing the thermal tolerance and gastric performance of a microbial phytase for use as a phosphate-mobilizing monogastric-feed supplement
-
Garrett JB, Kretz KA, O'Donoghue E, Kerovuo J, Kim W, et al. (2004) Enhancing the thermal tolerance and gastric performance of a microbial phytase for use as a phosphate-mobilizing monogastric-feed supplement. Appl Environ Microbiol 70: 3041-3046.
-
(2004)
Appl Environ Microbiol
, vol.70
, pp. 3041-3046
-
-
Garrett, J.B.1
Kretz, K.A.2
O'Donoghue, E.3
Kerovuo, J.4
Kim, W.5
-
25
-
-
48449095514
-
GLUE-IT and PEDEL-AA: new programmes for analyzing protein diversity in randomized libraries
-
Firth AE, Patrick WM, (2008) GLUE-IT and PEDEL-AA: new programmes for analyzing protein diversity in randomized libraries. Nucleic Acids Res 36: W281-285.
-
(2008)
Nucleic Acids Res
, vol.36
, pp. 281-285
-
-
Firth, A.E.1
Patrick, W.M.2
-
26
-
-
54349090614
-
Addressing the numbers problem in directed evolution
-
Reetz MT, Kahakeaw D, Lohmer R, (2008) Addressing the numbers problem in directed evolution. ChemBioChem 9: 1797-1804.
-
(2008)
ChemBioChem
, vol.9
, pp. 1797-1804
-
-
Reetz, M.T.1
Kahakeaw, D.2
Lohmer, R.3
-
27
-
-
77956228537
-
Phosphorothioate-based ligase-independent gene cloning (PLICing): An enzyme-free and sequence-independent cloning method
-
Blanusa M, Schenk A, Sadeghi H, Marienhagen J, Schwaneberg U, (2010) Phosphorothioate-based ligase-independent gene cloning (PLICing): An enzyme-free and sequence-independent cloning method. Anal Biochem 406: 141-146.
-
(2010)
Anal Biochem
, vol.406
, pp. 141-146
-
-
Blanusa, M.1
Schenk, A.2
Sadeghi, H.3
Marienhagen, J.4
Schwaneberg, U.5
-
28
-
-
68049106179
-
Directed evolution drives the next generation of biocatalysts
-
Turner NJ, (2009) Directed evolution drives the next generation of biocatalysts. Nat Chem Biol 5: 567-573.
-
(2009)
Nat Chem Biol
, vol.5
, pp. 567-573
-
-
Turner, N.J.1
-
29
-
-
73149119826
-
Ultrahigh-throughput FACS-based screening for directed enzyme evolution
-
Yang G, Withers SG, (2009) Ultrahigh-throughput FACS-based screening for directed enzyme evolution. ChemBioChem 10: 2704-2715.
-
(2009)
ChemBioChem
, vol.10
, pp. 2704-2715
-
-
Yang, G.1
Withers, S.G.2
-
30
-
-
22144485602
-
Expanding the range of substrate acceptance of enzymes: combinatorial active-site saturation test
-
Reetz MT, Bocola M, Carballeira JD, Zha D, Vogel A, (2005) Expanding the range of substrate acceptance of enzymes: combinatorial active-site saturation test. Angew Chem, Int Ed Engl 44: 4192-4196.
-
(2005)
Angew Chem, Int Ed Engl
, vol.44
, pp. 4192-4196
-
-
Reetz, M.T.1
Bocola, M.2
Carballeira, J.D.3
Zha, D.4
Vogel, A.5
-
31
-
-
47549095527
-
Modular construction of plasmids through ligation-free assembly of vector components with oligonucleotide linkers
-
Vroom JA, Wang CL, (2008) Modular construction of plasmids through ligation-free assembly of vector components with oligonucleotide linkers. BioTechniques 44: 924-926.
-
(2008)
BioTechniques
, vol.44
, pp. 924-926
-
-
Vroom, J.A.1
Wang, C.L.2
-
33
-
-
13244269857
-
Multi-line split DNA synthesis: a novel combinatorial method to make high quality peptide libraries
-
Tabuchi I, Soramoto S, Ueno S, Husimi Y, (2004) Multi-line split DNA synthesis: a novel combinatorial method to make high quality peptide libraries. BMC Biotechnol 4: 19.
-
(2004)
BMC Biotechnol
, vol.4
, pp. 19
-
-
Tabuchi, I.1
Soramoto, S.2
Ueno, S.3
Husimi, Y.4
-
34
-
-
78049450070
-
Synthetic genomes for synthetic biology
-
Wang HH, (2010) Synthetic genomes for synthetic biology. J Mol Cell Biol 2: 178-179.
-
(2010)
J Mol Cell Biol
, vol.2
, pp. 178-179
-
-
Wang, H.H.1
-
35
-
-
0024283277
-
DNA and RNA sequence determination based on phosphorothioate chemistry
-
Gish G, Eckstein F, (1988) DNA and RNA sequence determination based on phosphorothioate chemistry. Science 240: 1520-1522.
-
(1988)
Science
, vol.240
, pp. 1520-1522
-
-
Gish, G.1
Eckstein, F.2
-
36
-
-
0025675856
-
High efficiency transformation of Escherichia coli with plasmids
-
Inoue H, Nojima H, Okayama H, (1990) High efficiency transformation of Escherichia coli with plasmids. Gene 96: 23-28.
-
(1990)
Gene
, vol.96
, pp. 23-28
-
-
Inoue, H.1
Nojima, H.2
Okayama, H.3
-
37
-
-
0004136246
-
Molecular Cloning: A Laboratory Manual
-
Cold Spring Harbor, N.Y, Cold Spring Harbor Laboratory Press
-
Maniatis T, Fritsch EF, Sambrook J, (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, N.Y Cold Spring Harbor Laboratory Press pp. 1-545.
-
(1982)
, pp. 1-545
-
-
Maniatis, T.1
Fritsch, E.F.2
Sambrook, J.3
-
38
-
-
0026038362
-
Plasmid transformation of Escherichia coli and other bacteria
-
Hanahan D, Jessee J, Bloom FR, (1991) Plasmid transformation of Escherichia coli and other bacteria. Methods Enzymol 204: 63-113.
-
(1991)
Methods Enzymol
, vol.204
, pp. 63-113
-
-
Hanahan, D.1
Jessee, J.2
Bloom, F.R.3
|