Exponential Megapriming PCR (EMP) Cloning-Seamless DNA Insertion into Any Target Plasmid without Sequence Constraints

PLoS ONE

Volumn 7, Issue 12, 2012, Pages

  Ulrich, Alexander ^a,b   Andersen, Kasper R ^a   Schwartz, Thomas U ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b FREIE UNIVERSITÄT BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT;

ARTICLE; EXPONENTIAL MEGAPRIMING POLYMERASE CHAIN REACTION CLONING; GENE AMPLIFICATION; GENE INSERTION; GENE SEQUENCE; INTERMETHOD COMPARISON; MOLECULAR CLONING; PLASMID; POLYMERASE CHAIN REACTION; PROCESS OPTIMIZATION; RESTRICTION FREE CLONING;

CLONING, MOLECULAR; DNA; GENETIC VECTORS; PLASMIDS; POLYMERASE CHAIN REACTION;

EID: 84871723830     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0053360     Document Type: Article

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