Exploring the potential of megaprimer PCR in conjunction with orthogonal array design for mutagenesis library construction

Biotechnology and Applied Biochemistry

Volumn 60, Issue 2, 2013, Pages 190-195

  Tang, Lixia ^a   Zheng, Kai ^a   Liu, Yu ^a   Zheng, Huayu ^a   Wang, Hu ^a   Song, Chunlei ^a   Zhou, Hong ^a  

^a UNIVERSITY OF ELECTRONIC SCIENCE AND TECHNOLOGY OF CHINA   (China)

Author keywords

mutagenesis efficiency; orthogonal array; PCR optimization

Indexed keywords

DIRECTED EVOLUTION; HALOHYDRIN DEHALOGENASE; HIGH QUALITY; MEGAPRIMER PCR; ORTHOGONAL ARRAY; ORTHOGONAL ARRAY DESIGNS; RANDOM MUTAGENESIS; SATURATION MUTAGENESIS;

LIBRARIES; OPTIMIZATION;

MUTAGENESIS;

DNA POLYMERASE; MAGNESIUM ION; PRIMER DNA;

ANALYTICAL EQUIPMENT; ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; DNA LIBRARY; DNA TEMPLATE; GENE; MEGAPRIMER POLYMERASE CHAIN REACTION; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; ORTHOGONAL ARRAY DESIGN; POLYMERASE CHAIN REACTION; PROCESS DESIGN; PROCESS OPTIMIZATION; RANDOMIZATION; RHIZOBIUM RADIOBACTER; WILD TYPE;

BASE SEQUENCE; DNA PRIMERS; MUTAGENESIS; POLYMERASE CHAIN REACTION;

EID: 84876471612     PISSN: 08854513     EISSN: 14708744     Source Type: Journal    
DOI: 10.1002/bab.1065     Document Type: Article

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