Restriction enzyme-free construction of random gene mutagenesis libraries in Escherichia coli

Analytical Biochemistry

Volumn 421, Issue 2, 2012, Pages 640-648

  Pai, Jen C ^a   Entzminger, Kevin C ^a   Maynard, Jennifer A ^a  

^a The University of Texas at Austin   (United States)

Author keywords

Directed evolution; Error prone PCR; Kunkel mutagenesis; Megaprimers; Protein engineering

Indexed keywords

BINDING ENERGY; DNA; ENZYMES; ESCHERICHIA COLI; GENES; LIBRARIES; MUTAGENESIS;

DIRECTED EVOLUTION; ENGINEERING APPLICATIONS; ERROR PRONE PCR; LIGAND BINDING AFFINITY; MEGAPRIMERS; PROTEIN ENGINEERING; SINGLE-CHAIN ANTIBODIES; SITE DIRECTED MUTAGENESIS;

POLYMERASE CHAIN REACTION;

POLYDEOXYRIBONUCLEOTIDE SYNTHASE; RESTRICTION ENDONUCLEASE;

ARTICLE; ESCHERICHIA COLI; GENE LIBRARY; GENE TARGETING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS;

ESCHERICHIA COLI;

EID: 84859707637     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.11.009     Document Type: Article

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