Efficient screening of fungal cellobiohydrolase class i enzymes for thermostabilizing sequence blocks by SCHEMA structure-guided recombination

Protein Engineering, Design and Selection

Volumn 23, Issue 11, 2010, Pages 871-880

  Heinzelman, Pete ^a   Komor, Russell ^a   Kanaan, Arvind ^a   Romero, Philip ^a   Yu, Xinlin ^a   Mohler, Shannon ^a   Snow, Christopher ^a   Arnold, Frances ^a  

^a California Institute of Technology   (United States)

Author keywords

biofuel; CBH I; Cel7A; cellulase; directed evolution; protein recombination; protein thermostability

Indexed keywords

AMINO ACID SEQUENCE; APPLICATION-SPECIFIC; CBH I; CEL7A; CELLOBIOHYDROLASES; CELLULASE; CLASS I; DIRECTED EVOLUTION; NATURAL ENZYME; TALAROMYCES EMERSONII; THERMOPHILIC FUNGUS; THERMOSTABLE CELLOBIOHYDROLASE;

AMINO ACIDS; BIOFUELS; CATALYSTS;

ENZYMES;

AMINO ACID; CELLOBIOHYDROLASE CLASS I; CELLULOSE; CELLULOSE 1,4 BETA CELLOBIOSIDASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CHIMERA; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ENGINEERING; ENZYME STRUCTURE; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; SCHEMA STRUCTURE GUIDED RECOMBINATION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; TALAROMYCES; THERMOSTABILITY;

AMINO ACID SEQUENCE; CELLULOSE; CELLULOSE 1,4-BETA-CELLOBIOSIDASE; ENZYME STABILITY; FUNGI; MODELS, MOLECULAR; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TEMPERATURE;

FUNGI; TALAROMYCES EMERSONII;

EID: 77958179580     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzq063     Document Type: Article

References (17)

1. Amin, N., Liu, A. D., Ramer, S., Aehle, W., Meijer, D., Metin, M., Wong, S., Gualfetti, P. and Schellenberger, V. (2004) Protein Eng. Des. Sel., 17, 787-793.
2. Den Haan, R., Rose, S. H., Lynd, L. R. and van Zyl, W. H. (2007) Metab. Eng., 9, 87-94.
3. Endelman, J. B., Silberg, J. J., Wang, Z. G. and Arnold, F. H. (2004) Protein Eng. Des. Sel., 17, 589-594.
4. Godbole, S., Decker, S. R., Nieves, R. A., Adney, W. S., Vinzant, T. B., Baker, J. O., Thomas, S. R. and Himmel, M. E. (1999) Biotechnol. Prog., 15, 828-833.
5. Grassick, A., Murray, P. G., Thompson, R., Collins, C. M., Byrnes, L., Birrane, G., Higgins, T. M. and Tuohy, M. G. (2004) Eur. J. Biochem., 271, 4495-4506.
6. Gusakov, A. V., Salanovich, T. N., Antonov, A. I., Ustinov, B. B., Okunev, O. N., Burlingame, R., Emalfarb, M., Baez, M. and Sinitsyn, A. P. (2007) Biotechnol. Bioeng., 97, 1028-1038.
7. Heinzelman, P., Snow, C. D., Smith, M. A., Yu, X., Kanaan, A., Boulware, K., Villalobos, A., Govindarajan, S., Minshull, J. and Arnold, F. H. (2009a) J. Biol. Chem., 284, 26229-26233.
8. Heinzelman, P., Snow, C. D., Wu, I., Nguyen, C., Villalobos, A., Govindarajan, S., Minshull, J. and Arnold, F. H. (2009b) Proc. Natl Acad. Sci. USA, 106, 5610-5615.
9. Jäckel, C., Bloom, J. D., Kast, P., Arnold, F. H. and Hilvert, D. (2010) J. Mol. Biol., 399, 541-546.
10. Le Crom, S., Schackwitz, W., Pennacchio, L., et al. (2009) Proc. Natl Acad. Sci. USA, 106, 16151-16156.
11. Lehmann, M., Loch, C., Middendorf, A., Studer, D., Lassen, S. F., Pasamontes, L., van Loon, A. P. and Wyss, M. (2002) Protein Eng., 15, 403-411.
12. Li, Y., Drummond, D. A., Sawayama, A. M., Snow, C. D., Bloom, J. D. and Arnold, F. H. (2007) Nat. Biotechnol., 25, 1051-1056.
13. Meyer, M. M., Hochrein, L. and Arnold, F. H. (2006) Protein Eng. Des. Sel., 19, 563-570.
14. Viikari, L., Alapuranen, M., Puranen, T., Vehmaanpera, J. and Siika-aho, M. (2007) Adv. Biocheml. Eng. Biotechnol., 108, 121-145.
15. Voutilainen, S. P., Puranen, T., Siika-Aho, M., et al. (2008) Biotechnol. Bioeng., 101, 515-528.
16. Voutilainen, S. P., Boer, H., Alapuranen, M., Jänis, J., Vehmaanperä, J. and Koivula, A. (2009) Appl. Microbiol. Biotechnol., 83, 261-272.
17. Voutilainen, S. P., Murray, P. G., Tuohy, M. G. and Koivula, A. (2010) Protein Eng. Des. Sel., 23, 69-79.