STRU-cloning: A fast, inexpensive and efficient cloning procedure applicable to both small scale and structural genomics size cloning

Molecular Biotechnology

Volumn 48, Issue 1, 2011, Pages 30-37

  Bellini, Dom ^a   Fordham Skelton, Anthony P ^b   Papiz, Miroslav Z ^a  

^a UNIVERSITY OF LIVERPOOL   (United Kingdom)

^b DARESBURY LABORATORY   (United Kingdom)

Author keywords

Automation; DNA gel extraction; Gene cloning; High throughput cloning; Ligation dependent cloning; Ligation independent cloning; Restriction enzymes; STRU cloning

Indexed keywords

GENE CLONING; HIGH-THROUGHPUT; LIGATION-DEPENDENT CLONING; LIGATION-INDEPENDENT CLONING; RESTRICTION ENZYMES; STRU-CLONING;

AUTOMATION; CATALYSTS; CENTRIFUGATION; DNA; ELECTROPHORESIS; ENZYMES; GELS; GENES; POLYMERASE CHAIN REACTION; ULTRAFILTRATION;

CLONING;

DNA FRAGMENT;

ANTIBIOTIC RESISTANCE; ARTICLE; AUTOMATION; DNA PURIFICATION; ESCHERICHIA COLI; GENE INSERTION; GENE SYNTHESIS; GENOMICS; MOLECULAR CLONING; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; SINGLE TUBE RESTRICTION BASED ULTRAFILTRATION CLONING;

CLONING, MOLECULAR; DNA; REPRODUCIBILITY OF RESULTS;

EID: 79955914893     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-010-9345-7     Document Type: Article

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