-
1
-
-
0028050350
-
Rapid evolution of a protein in vitro by DNA shuffling
-
Stemmer W.P. Rapid evolution of a protein in vitro by DNA shuffling. Nature 1994, 370:389-391.
-
(1994)
Nature
, vol.370
, pp. 389-391
-
-
Stemmer, W.P.1
-
2
-
-
0028110130
-
DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution
-
Stemmer W.P. DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution. Proc. Natl. Acad. Sci. U. S. A. 1994, 91:10747-10751.
-
(1994)
Proc. Natl. Acad. Sci. U. S. A.
, vol.91
, pp. 10747-10751
-
-
Stemmer, W.P.1
-
3
-
-
0032518266
-
DNA shuffling of a family of genes from diverse species accelerates directed evolution
-
Crameri A., et al. DNA shuffling of a family of genes from diverse species accelerates directed evolution. Nature 1998, 391:288-291.
-
(1998)
Nature
, vol.391
, pp. 288-291
-
-
Crameri, A.1
-
4
-
-
0028088274
-
The progeny of sexual PCR
-
Smith G.P. The progeny of sexual PCR. Nature 1994, 370:324-325.
-
(1994)
Nature
, vol.370
, pp. 324-325
-
-
Smith, G.P.1
-
5
-
-
20444368826
-
Laboratory evolution of catabolic enzymes and pathways
-
Parales R.E., Ditty J.L. Laboratory evolution of catabolic enzymes and pathways. Curr. Opin. Biotechnol. 2005, 16:315-325.
-
(2005)
Curr. Opin. Biotechnol.
, vol.16
, pp. 315-325
-
-
Parales, R.E.1
Ditty, J.L.2
-
6
-
-
24944455200
-
Laboratory-directed protein evolution
-
Yuan L., et al. Laboratory-directed protein evolution. Microbiol. Mol. Biol. Rev. 2005, 69:373-392.
-
(2005)
Microbiol. Mol. Biol. Rev.
, vol.69
, pp. 373-392
-
-
Yuan, L.1
-
7
-
-
33748339568
-
Mutant library construction in directed molecular evolution: casting a wider net
-
Wang T.W., et al. Mutant library construction in directed molecular evolution: casting a wider net. Mol. Biotechnol. 2006, 34:55-68.
-
(2006)
Mol. Biotechnol.
, vol.34
, pp. 55-68
-
-
Wang, T.W.1
-
8
-
-
0033964975
-
An effective family shuffling method using single-stranded DNA
-
Kikuchi M., et al. An effective family shuffling method using single-stranded DNA. Gene 2000, 243:133-137.
-
(2000)
Gene
, vol.243
, pp. 133-137
-
-
Kikuchi, M.1
-
9
-
-
0032814117
-
Novel family shuffling methods for the in vitro evolution of enzymes
-
Kikuchi M., et al. Novel family shuffling methods for the in vitro evolution of enzymes. Gene 1999, 236:159-167.
-
(1999)
Gene
, vol.236
, pp. 159-167
-
-
Kikuchi, M.1
-
10
-
-
0031855459
-
When blind is better: protein design by evolution
-
Arnold F.H. When blind is better: protein design by evolution. Nat. Biotechnol. 1998, 16:617-618.
-
(1998)
Nat. Biotechnol.
, vol.16
, pp. 617-618
-
-
Arnold, F.H.1
-
11
-
-
0032787560
-
'Itching' for new strategies in protein engineering
-
Michnick S.W., Arnold F.H. 'Itching' for new strategies in protein engineering. Nat. Biotechnol. 1999, 17:1159-1160.
-
(1999)
Nat. Biotechnol.
, vol.17
, pp. 1159-1160
-
-
Michnick, S.W.1
Arnold, F.H.2
-
12
-
-
0037114242
-
Random DNA fragmentation with endonuclease V: application to DNA shuffling
-
Miyazaki K. Random DNA fragmentation with endonuclease V: application to DNA shuffling. Nucleic Acids Res. 2002, 30:e139.
-
(2002)
Nucleic Acids Res.
, vol.30
-
-
Miyazaki, K.1
-
13
-
-
0034667190
-
High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2
-
Abécassis V., et al. High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2. Nucleic Acids Res. 2000, 28:e88.
-
(2000)
Nucleic Acids Res.
, vol.28
-
-
Abécassis, V.1
-
14
-
-
58949085244
-
An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol
-
Liu H., Naismith J.H. An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol. BMC Biotechnol. 2008, 8:91.
-
(2008)
BMC Biotechnol.
, vol.8
, pp. 91
-
-
Liu, H.1
Naismith, J.H.2
-
15
-
-
0029684150
-
Site-directed mutagenesis using double-stranded plasmid DNA templates
-
Braman J., et al. Site-directed mutagenesis using double-stranded plasmid DNA templates. Methods Mol. Biol. 1996, 57:31-44.
-
(1996)
Methods Mol. Biol.
, vol.57
, pp. 31-44
-
-
Braman, J.1
-
16
-
-
0036777652
-
One-step site-directed mutagenesis of ATM cDNA in large (20kb) plasmid constructs
-
Scott S.P., et al. One-step site-directed mutagenesis of ATM cDNA in large (20kb) plasmid constructs. Hum. Mutat. 2002, 20:323.
-
(2002)
Hum. Mutat.
, vol.20
, pp. 323
-
-
Scott, S.P.1
-
17
-
-
26244438851
-
A single-stage polymerase-based protocol for the introduction of deletions and insertions without subcloning
-
Salerno J.C., et al. A single-stage polymerase-based protocol for the introduction of deletions and insertions without subcloning. Mol. Biotechnol. 2005, 29:225-232.
-
(2005)
Mol. Biotechnol.
, vol.29
, pp. 225-232
-
-
Salerno, J.C.1
-
18
-
-
36249032614
-
A one-tube site-directed mutagenesis method using PCR and primer extension
-
Chapnik N., et al. A one-tube site-directed mutagenesis method using PCR and primer extension. Anal. Biochem. 2008, 372:255-257.
-
(2008)
Anal. Biochem.
, vol.372
, pp. 255-257
-
-
Chapnik, N.1
-
19
-
-
37549056267
-
Site-directed mutagenesis by combination of homologous recombination and DpnI digestion of the plasmid template in Escherichia coli
-
Li J., et al. Site-directed mutagenesis by combination of homologous recombination and DpnI digestion of the plasmid template in Escherichia coli. Anal. Biochem. 2008, 373:389-391.
-
(2008)
Anal. Biochem.
, vol.373
, pp. 389-391
-
-
Li, J.1
-
20
-
-
40649107834
-
A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method
-
Tseng W.C., et al. A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method. Anal. Biochem. 2008, 375:376-378.
-
(2008)
Anal. Biochem.
, vol.375
, pp. 376-378
-
-
Tseng, W.C.1
-
21
-
-
41149148236
-
Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts
-
Klock H.E., et al. Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts. Proteins 2008, 71:982-994.
-
(2008)
Proteins
, vol.71
, pp. 982-994
-
-
Klock, H.E.1
-
22
-
-
0036134273
-
Two-stage polymerase chain reaction protocol allowing introduction of multiple mutations, deletions, and insertions, using QuikChange site-directed mutagenesis
-
Wang W., Malcolm B.A. Two-stage polymerase chain reaction protocol allowing introduction of multiple mutations, deletions, and insertions, using QuikChange site-directed mutagenesis. Methods Mol. Biol. 2002, 182:37-43.
-
(2002)
Methods Mol. Biol.
, vol.182
, pp. 37-43
-
-
Wang, W.1
Malcolm, B.A.2
-
23
-
-
2342478578
-
Generation of eight adjacent mutations in a single step using a site-directed mutagenesis kit
-
Cabré A., et al. Generation of eight adjacent mutations in a single step using a site-directed mutagenesis kit. Clin. Chem. Lab. Med. 2004, 42:384-386.
-
(2004)
Clin. Chem. Lab. Med.
, vol.42
, pp. 384-386
-
-
Cabré, A.1
-
24
-
-
42649089776
-
Single-step overlap-primer-walk polymerase chain reaction for multiple mutagenesis without overlap extension
-
Kumar R., Rajagopal K. Single-step overlap-primer-walk polymerase chain reaction for multiple mutagenesis without overlap extension. Anal. Biochem. 2008, 377:105-107.
-
(2008)
Anal. Biochem.
, vol.377
, pp. 105-107
-
-
Kumar, R.1
Rajagopal, K.2
-
25
-
-
3843146246
-
An efficient one-step site-directed and site-saturation mutagenesis protocol
-
Zheng L., et al. An efficient one-step site-directed and site-saturation mutagenesis protocol. Nucleic Acids Res. 2004, 32:e115.
-
(2004)
Nucleic Acids Res.
, vol.32
-
-
Zheng, L.1
-
26
-
-
33744475011
-
Directed evolution of enantioselective enzymes: iterative cycles of CASTing for probing protein-sequence space
-
Reetz M.T., et al. Directed evolution of enantioselective enzymes: iterative cycles of CASTing for probing protein-sequence space. Angew. Chem. Int. Ed. Engl. 2006, 45:1236-1241.
-
(2006)
Angew. Chem. Int. Ed. Engl.
, vol.45
, pp. 1236-1241
-
-
Reetz, M.T.1
-
27
-
-
79958023522
-
Fine-tuning enzyme activity through saturation mutagenesis
-
Hogrefe H.H. Fine-tuning enzyme activity through saturation mutagenesis. Methods Mol. Biol. 2010, 634:271-283.
-
(2010)
Methods Mol. Biol.
, vol.634
, pp. 271-283
-
-
Hogrefe, H.H.1
-
28
-
-
1842842876
-
Creating random mutagenesis libraries using megaprimer PCR of whole plasmid
-
1036-1038
-
Miyazaki K., Takenouchi M. Creating random mutagenesis libraries using megaprimer PCR of whole plasmid. Biotechniques 2002, 33:1033-1034. 1036-1038.
-
(2002)
Biotechniques
, vol.33
, pp. 1033-1034
-
-
Miyazaki, K.1
Takenouchi, M.2
-
29
-
-
0036841628
-
Creating randomized amino acid libraries with the QuikChange multi site-directed mutagenesis kit
-
1162, 1164-1165
-
Hogrefe H.H., et al. Creating randomized amino acid libraries with the QuikChange multi site-directed mutagenesis kit. Biotechniques 2002, 33:1158-1160. 1162, 1164-1165.
-
(2002)
Biotechniques
, vol.33
, pp. 1158-1160
-
-
Hogrefe, H.H.1
-
30
-
-
0034947940
-
Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase
-
92
-
Geiser M., et al. Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase. Biotechniques 2001, 31:88-90. 92.
-
(2001)
Biotechniques
, vol.31
, pp. 88-90
-
-
Geiser, M.1
-
31
-
-
77955506174
-
A QuikChange-like method to realize efficient blunt-ended DNA directional cloning and site-directed mutagenesis simultaneously
-
An Y., et al. A QuikChange-like method to realize efficient blunt-ended DNA directional cloning and site-directed mutagenesis simultaneously. Biochem. Biophys. Res. Commun. 2010, 397:136-139.
-
(2010)
Biochem. Biophys. Res. Commun.
, vol.397
, pp. 136-139
-
-
An, Y.1
-
32
-
-
57349088820
-
Sequence-independent construction of ordered combinatorial libraries with predefined crossover points
-
Jézéquel L., et al. Sequence-independent construction of ordered combinatorial libraries with predefined crossover points. Biotechniques 2008, 45:523-532.
-
(2008)
Biotechniques
, vol.45
, pp. 523-532
-
-
Jézéquel, L.1
-
33
-
-
0038731100
-
Library analysis of SCHEMA-guided protein recombination
-
Meyer M.M., et al. Library analysis of SCHEMA-guided protein recombination. Protein Sci. 2003, 12:1686-1693.
-
(2003)
Protein Sci.
, vol.12
, pp. 1686-1693
-
-
Meyer, M.M.1
-
34
-
-
0038799745
-
General method for sequence-independent site-directed chimeragenesis
-
Hiraga K., Arnold F.H. General method for sequence-independent site-directed chimeragenesis. J. Mol. Biol. 2003, 330:287-296.
-
(2003)
J. Mol. Biol.
, vol.330
, pp. 287-296
-
-
Hiraga, K.1
Arnold, F.H.2
-
35
-
-
0036307433
-
Analysis of shuffled gene libraries
-
Joern J.M., et al. Analysis of shuffled gene libraries. J. Mol. Biol. 2002, 316:643-656.
-
(2002)
J. Mol. Biol.
, vol.316
, pp. 643-656
-
-
Joern, J.M.1
-
36
-
-
38349129109
-
Revealing biases inherent in recombination protocols
-
Chaparro-Riggers J.F., et al. Revealing biases inherent in recombination protocols. BMC Biotechnol. 2007, 7:77.
-
(2007)
BMC Biotechnol.
, vol.7
, pp. 77
-
-
Chaparro-Riggers, J.F.1
-
38
-
-
0028804911
-
Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli
-
Kane J.F. Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli. Curr. Opin. Biotechnol. 1995, 6:494-500.
-
(1995)
Curr. Opin. Biotechnol.
, vol.6
, pp. 494-500
-
-
Kane, J.F.1
-
39
-
-
33750432194
-
Rare codon clusters at 5'-end influence heterologous expression of archaeal gene in Escherichia coli
-
Kim S., Lee S.B. Rare codon clusters at 5'-end influence heterologous expression of archaeal gene in Escherichia coli. Protein Exp. Purif. 2006, 50:49-57.
-
(2006)
Protein Exp. Purif.
, vol.50
, pp. 49-57
-
-
Kim, S.1
Lee, S.B.2
-
40
-
-
0029670577
-
Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents
-
Moore J.C., Arnold F.H. Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents. Nat. Biotechnol. 1996, 14:458-467.
-
(1996)
Nat. Biotechnol.
, vol.14
, pp. 458-467
-
-
Moore, J.C.1
Arnold, F.H.2
|