A method for multi-codon scanning mutagenesis of proteins based on asymmetric transposons

Protein Engineering, Design and Selection

Volumn 25, Issue 2, 2012, Pages 67-72

  Liu, Jia ^a   Cropp, T Ashton ^a  

^a VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

Author keywords

codon mutagenesis; directed evolution; transposon

Indexed keywords

DIRECTED EVOLUTION; PROTEIN FUNCTIONS; PROTEIN SEQUENCES; RANDOM MUTAGENESIS; SINGLE EXCITATION; SITE PREFERENCES; TRANSPOSON; UV REGION;

AMINO ACIDS; GENE ENCODING; PROTEINS;

MUTAGENESIS;

OLIGONUCLEOTIDE;

AMINO ACID SEQUENCE; ARTICLE; CLONE; CODON; DNA LIBRARY; GENE LIBRARY; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN FUNCTION; SCREENING; TRANSPOSON;

BASE SEQUENCE; CLONING, MOLECULAR; CODON; DIRECTED MOLECULAR EVOLUTION; DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; PROTEIN ENGINEERING;

EID: 84863060412     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzr060     Document Type: Article

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