High-throughput cloning of human liver complete open reading frames using homologous recombination in Escherichia coli

Analytical Biochemistry

Volumn 397, Issue 2, 2010, Pages 162-167

  Zhu, Dewu ^a   Zhong, Xin ^a   Tan, Rong ^b   Chen, Liang ^a   Huang, Guangrui ^a   Li, Jing ^a   Sun, Xiaoyan ^a   Xu, Li ^a   Chen, Jin ^a   Ou, Yangping ^a   Zhang, Ting ^a   Yuan, Dongxia ^a   Zhang, Zhen ^a   Shu, Wangyun ^a   Ma, Lixin ^a  

^a HUBEI UNIVERSITY   (China)

^b NANTONG UNIVERSITY   (China)

Author keywords

Genome; High throughput; Homologous recombination; Proteome; Seamless

Indexed keywords

CLONE CELLS; CLONING; ESCHERICHIA COLI; GENES; LINEARIZATION; PROTEINS; VECTORS;

EXPRESSION VECTORS; FLANKING SEQUENCE; HIGH THROUGHPUT; HOMOLOGOUS RECOMBINATION; OPEN READING FRAME; PCR AMPLIFICATION; SEAMLESS; SPECIFIC PRIMERS;

POLYMERASE CHAIN REACTION;

DNA FRAGMENT;

ARTICLE; CONTROLLED STUDY; DNA FLANKING REGION; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE SEQUENCE; HIGH THROUGHPUT SCREENING; HOMOLOGOUS RECOMBINATION; HUMAN; LIVER; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; SEAMLESS ENZYME FREE CLONING;

ESCHERICHIA COLI;

EID: 72049132108     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.10.018     Document Type: Article

References (23)

1. Cyranoski D. China takes center stage for liver proteome. Nature 425 (2003) 441
2. Service R.F. Public projects gear up to chart the protein landscape. Science 302 (2003) 1316-1318
3. He F. Human liver proteome project: plan, progress, and perspectives. Mol. Cell. Proteomics 4 (2005) 1841-1848
4. Aslanidis C., and deJong P.J. Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res. 18 (1990) 6069-6074
5. Hsiao K. Exonuclease III induced ligase-free directional subcloning of PCR products. Nucleic Acids Res. 21 (1993) 5528-5529
6. Bubeck P., Winkler M., and Bautsch W. Rapid cloning by homologous recombination in vivo. Nucleic Acids Res. 21 (1993) 3601-3602
7. Liu Q., Li M.Z., Leibham D., Cortez D., and Elledge S.J. The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes. Curr. Biol. 8 (1998) 1300-1309
8. Oliner J.D., Kinzler K.W., and Vogelstein B. In vivo cloning of PCR products in E. coli. Nucleic Acids Res. 21 (1993) 5192-5197
9. Hartley J.L., Temple G.F., and Brasch M.A. DNA cloning using in vitro site-specific recombination. Genome Res. 10 (2000) 1788-1795
10. Walhout A.J., Temple G.F., Brasch M.A., Hartley J.L., Lorson M.A., van den Heuvel S., and Vidal M. GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes. Methods Enzymol. 328 (2000) 575-592
11. Fu C., Wehr D.R., Edwards J., and Hauge B. Rapid one-step recombinational cloning. Nucleic Acids Res. 36 (2008) e54
12. Li M.Z., and Elledge S.J. MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules. Nat. Genet. 37 (2005) 311-319
13. Siegel R.W., Velappan N., Pavlik P., Chasteen L., and Bradbury A. Recombinatorial cloning using heterologous Lox sites. Genome Res. 14 (2004) 1119-1129
14. Zhu H., Bilgin M., Bangham R., Hall D., Casamayor A., Bertone P., Lan N., Jansen R., Bidlingmaier S., and Houfek T. Global analysis of protein activities using proteome chips. Science 293 (2001) 2101-2105
15. Berrow N.S., Alderton D., Sainsbury S., Nettleship J., Assenberg R., Rahman N., Stuart D.I., and Owens R.J. A versatile ligation-independent cloning method suitable for high-throughput expression screening applications. Nucleic Acids Res. 35 (2007) e45
16. Zhu B., Cai G., Hall E.O., and Freeman G.J. In-fusion assembly: seamless engineering of multidomain fusion proteins. BioTechniques 43 (2007) 354-359
17. Marsischky G., and LaBaer J. Many paths to many clones: a comparative look at high-throughput cloning methods. Genome Res. 14 (2004) 2020-2028
18. Suzuki J.Y., Sriraman P., Svab Z., and Maliga P. Unique architecture of the plastid ribosomal RNA operon promoter recognized by the multisubunit RNA polymerase in tobacco and other higher plants. Plant Cell 15 (2003) 195-205
19. Inoue H., Nojima H., and Okayama H. High efficiency transformation of Escherichia coli with plasmids. Gene 96 (1990) 23-28
20. Xu J., Zeng J., Wan G., Hu G., Yan H., and Ma L. Construction of siRNA/miRNA expression vectors based on a one-step PCR process. BMC Biotechnol. 9 (2009) 53
21. Dutra B.E., Sutera Jr. V.A., and Lovett S.T. RecA-independent recombination is efficient but limited by exonucleases. Proc. Natl. Acad. Sci. USA 104 (2007) 216-221
22. Li J., Li C., Xiao W., Yuan D., Wan G., and Ma L. Site-directed mutagenesis by combination of homologous recombination and DpnI digestion of the plasmid template in Escherichia coli. Anal. Biochem. 373 (2008) 389-391
23. Tan R., Li C., Jiang S., and Ma L. A novel, simple method for construction of recombinant adenoviruses. Nucleic Acids Res. 34 (2006) e89