Two-stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChange(TM) Site-Directed Mutagenesis

BioTechniques

Volumn 26, Issue 4, 1999, Pages 680-682

  Wang, Wenyan ^a   Malcolm, Bruce A ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DNA SEQUENCE; GENE DELETION; GENE INSERTION; POINT MUTATION; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS;

EID: 0032957639     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99264st03     Document Type: Article

References (7)

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2. Ke, S.H. and E.L. Madison. 1997. Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method. Nucleic Acids Res. 25:3371-3372.
3. Mullins, K.B. and F.A. Faloona. 1987. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 155:335-350.
4. Mullis, K., F. Faloona, S. Scharf, R. Saiki, G. Horn and H. Erlich. 1986. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harb. Symp. Quant. Biol. 51 Pt 1:263-273.
5. Parikh, A. and F.P. Guengerich. 1998. Random mutagenesis by whole-plasmid PCR amplification. BioTechniques 24:428-431.
6. Shen, T.J., L.Q. Zhu and X. Sun. 1991. A marker-coupled method for site-directed mutagenesis. Gene 103:13-11.
7. Silver, J., T. Limjoco and S. Feinstone. 1995. Site-specific mutagenesis using the polymerase chain reaction, p. 179-180, PCR Strategies. Academic Press, San Diego.