Altering coenzyme specificity of Pichia stipitis xylose reductase by the semi-rational approach CASTing

Microbial Cell Factories

Volumn 6, Issue , 2007, Pages

  Liang, Ling ^a   Zhang, Jingqing ^a,b   Lin, Zhanglin ^a,c  

^a TSINGHUA UNIVERSITY   (China)

^b Massachusetts Institute of Technology Cambridge   (United States)

^c NANJING TECH UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE; FUNGAL ENZYME; NITRATE REDUCTASE (NADPH); OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; UNCLASSIFIED DRUG; XYLOSE; XYLOSE REDUCTASE;

ARTICLE; CASTING; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SPECIFICITY; FUNGAL STRAIN; GENE MUTATION; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA STIPITIS; PREDICTION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN INTERACTION; SCREENING TEST; SEQUENCE HOMOLOGY; WILD TYPE; YEAST;

PICHIA STIPITIS; SACCHAROMYCES CEREVISIAE;

EID: 38649127524     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-6-36     Document Type: Article

References (32)

1. Kötter P Ciriacy M Xylose fermentation by Saccharomyces cerevisiae Appl Microbiol Biotechnol 1993, 38:776-783.
2. Zhang YY Lee H Site-directed mutagenesis of the cysteine residues in the Pichia stipitis xylose reductase FEMS Microbiol Lett 1997, 147(2):227-232. 9119198
3. Kostrzynska M Sopher CR Lee H Mutational analysis of the role of the conserved lysine-270 in the Pichia stipitis xylose reductase FEMS Microbiol Lett 1998, 159(1):107-112. 9485600
4. Watanabe S Kodaki T Makino K Complete reversal of coenzyme specificity of xylitol dehydrogenase and increase of thermostability by the introduction of structural zinc J Biol Chem 2005, 280(11):10340-10349. 15623532
5. m for NADPH increases ethanol production from xylose in recombinant Saccharomyces cerevisiae Biotechnol Bioeng 2006, 93(4):665-673. 16372361
6. Jez JM Bennett MJ Schlegel BP Lewis M Penning TM Comparative anatomy of the aldo-keto reductase superfamily Biochem J 1997, 326:625-636. 1218714 9307009
7. Lee H The structure and function of yeast xylose (aldose) reductases Yeast 1998, 14(11):977-984. 9730277
8. Petschacher B Leitgeb S Kavanagh KL Wilson DK Nidetzky B The coenzyme specificity of Candida tenuis xylose reductase (AKR2B5) explored by site-directed mutagenesis and X-ray crystallography Biochem J 2005, 385:75-83. 1134675 15320875
9. Kavanagh KL Klimacek M Nidetzky B Wilson DK Structure of xylose reductase bound to NAD(+) and the basis for single and dual co-substrate specificity in family 2 aldo-keto reductases Biochem J 2003, 373:319-326. 1223518 12733986
10. Reetz MT Directed evolution of enantioselective enzymes as catalysts for organic synthesis Advances in Catalysis SAN DIEGO, ELSEVIER ACADEMIC PRESS INC Gates B, Knözinger H 2006, 49:1-69.
11. Reetz MT D Carballeira J Vogel A Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability Angew Chem, Int Ed 2006, 45(46):7745-7751.
12. Reetz MT Wang LW Bocola M Directed evolution of enantioselective enzymes: Iterative cycles of CASTing for probing protein-sequence space Angew Chem, Int Ed 2006, 45(8):1236-1241.
13. Reetz MT Bocola M Carballeira JD Zha DX Vogel A Expanding the range of substrate acceptance of enzymes: Combinatorial active-site saturation test Angew Chem, Int Ed 2005, 44(27):4192-4196.
14. Kavanagh KL Klimacek M Nidetzky B Wilson DK The structure of apo and holo forms of xylose reductase, a dimeric aldo-keto reductase from Candida tenuis Biochemistry 2002, 41(28):8785-8795. 12102621
15. Skolnick J Kihara D Zhang Y Development and large scale benchmark testing of the PROSPECTOR_3 threading algorithm Proteins: Struct, Funct, Bioinf 2004, 56(3):502-518.
16. Zhang Y Skolnick J Automated structure prediction of weakly homologous proteins on a genomic scale Proc Natl Acad Sci U S A 2004, 101(20):7594-7599. 419651 15126668
17. Petschacher B Nidetzky B Engineering Candida tenuis xylose reductase for improved utilization of NADH: Antagonistic effects of multiple side chain replacements and performance of site-directed mutants under simulated in vivo conditions Appl Environ Microbiol 2005, 71(10):6390-6393. 1265968 16204564
18. Warren MS Benkovic SJ Combinatorial manipulation of three key active site residues in glycinamide ribonucleotide transformylase Protein Eng 1997, 10(1):63-68. 9051735
19. Reetz MT Carballeira JD Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes Nat Protocols 2007, 2(4):891-903.
20. Reetz MT Wilensek S Zha DX Jaeger KE Directed evolution of an enantioselective enzyme through combinatorial multiple-cassette mutagenesis Angew Chem, Int Ed 2001, 40(19):3589-3591.
21. Watanabe S Abu Saleh A Pack SP Annaluru N Kodaki T Makino K Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein-engineered NADH-preferring xylose reductase from Pichia stipitis Microbiology 2007, 153(9):3044-3054. 17768247
22. Woodyer R Simurdiak M van der Donk WA Zhao HM Heterologous expression, purification, and characterization of a highly active xylose reductase from Neurospora crassa Appl Environ Microbiol 2005, 71(3):1642-1647. 1065158 15746370
23. 2O-forming NADH oxidase and impact on redox metabolism Metab Eng 2006, 8(4):303-314. 16473032
24. Walfridsson M Anderlund M Bao X HahnHagerdal B Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and its effects on product formation during xylose utilisation Appl Microbiol Biotechnol 1997, 48(2):218-224. 9299780
25. Kuyper M Harhangi HR Stave AK Winkler AA Jetten MSM de Laat W den Ridder JJJ Op den Camp HJM van Dijken JP Pronk JT High-level functional expression of a fungal xylose isomerase: The key to efficient ethanolic fermentation of xylose by Saccharomyces cerevisiae? FEMS Yeast Res 2003, 4(1):69-78. 14554198
26. Amore R Kotter P Kuster C Ciriacy M Hollenberg CP Cloning and expression in Saccharomyces cerevisiae of the NAD(P)H-dependent xylose reductase-encoding gene (XYL1) from the xylose-assimilating yeast Pichia stipitis Gene 1991, 109(1):89-97. 1756986
27. Glieder A Meinhold P High-throughput screens based on NAD(P)H depletion Directed Enzyme Evolution: Screening and Selection Methods Totowa, NJ, Humana Press Inc Arnold FH, Georgiou G 2003, 230:157-170.
28. O'Hare HM Baerga-Ortiz A Popovic B Spencer JB Leadlay PF High-throughput mutagenesis to evaluate models of stereochemical control in ketoreductase domains from the erythromycin polyketide synthase Chem Biol 2006, 13(3):287-296. 16638534
29. Sapan CV Lundblad RL Price NC Colorimetric protein assay techniques Biotechnol Appl Biochem 1999, 29:99-108. 10075906
30. Humphrey W Dalke A Schulten K VMD: Visual molecular dynamics J Mol Graphics 1996, 14(1):33-38.
31. TASSER-Lite: Protein structure prediction and modeling tool (beta-release) http://cssb.biology.gatech.edu/skolnick/webservice/ tasserlite/index.html
32. Verduyn C Van Kleef R Frank J Schreuder H Van Dijken JP Scheffers WA Properties of the NAD(P)H-dependent xylose reductase from the xylose-fermenting yeast Pichia stipitis Biochem J 1985, 226(3):669-677. 1144764 3921014