Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution

Biotechnology and Bioengineering

Volumn 110, Issue 3, 2013, Pages 711-720

  Martinez, Ronny ^a,b   Jakob, Felix ^a   Tu, Ran ^c   Siegert, Petra ^d   Maurer, Karl Heinz ^d,e   Schwaneberg, Ulrich ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b JACOBS UNIVERSITY BREMEN   (Germany)

^c Chinese Academy of Sciences   (China)

^d HENKEL KGAA   (Germany)

^e AB Enzymes GmbH   (Germany)

Author keywords

Alkaline protease; Directed evolution; Low temperature; Thermostability

Indexed keywords

ACTIVE SITE; ALKALINE PROTEASE; AMINO ACID SUBSTITUTION; CHARGED AMINO ACIDS; DETECTION METHODS; DIRECTED EVOLUTION; EXPRESSION SYSTEM; HIGH THROUGHPUT SCREENING; LOW TEMPERATURES; MICROTITER PLATES; PROTEOLYTIC ACTIVITIES; RANDOM MUTAGENESIS; SPECIFIC ACTIVITY; STABILITY IMPROVEMENT; STABILITY PROPERTIES; STERICALLY DEMANDING; SUBTILIS; SUBTILISIN; THERMOSTABILITY;

BACTERIOLOGY; HYDROGEN BONDS; ITERATIVE METHODS; SOAPS (DETERGENTS); STABILITY;

AMINO ACIDS;

ALKALINE PROTEINASE; BACILLUS GIBSONII ALKALINE PROTEASE; BACTERIAL ENZYME; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; BACILLUS; BACILLUS GIBSONII; HALF LIFE TIME; HEAT TOLERANCE; HYDROGEN BOND; INCUBATION TEMPERATURE; LOW TEMPERATURE; MICROTITER PLATE ASSAY; MOLECULAR EVOLUTION; MOLECULAR INTERACTION; MUTAGENESIS; NONHUMAN; PROTEIN DEGRADATION; PROTEIN EXPRESSION; SURFACE PROPERTY; THERMOSTABILITY;

AMINO ACID SUBSTITUTION; BACILLUS; BACTERIAL PROTEINS; DIRECTED MOLECULAR EVOLUTION; ENDOPEPTIDASES; ENZYME STABILITY; KINETICS; MUTAGENESIS; PROTEIN STABILITY; TEMPERATURE;

BACILLUS GIBSONII;

EID: 84872657998     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24766     Document Type: Article

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