An asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy

BMC Biotechnology

Volumn 12, Issue , 2012, Pages

  Bi, Yanzhen ^a   Qiao, Xianfeng ^a   Hua, Zaidong ^a   Zhang, Liping ^a   Liu, Ximei ^a   Li, Li ^a   Hua, Wenjun ^a   Xiao, Hongwei ^a   Zhou, Jingrong ^a   Wei, Qingxin ^a   Zheng, Xinmin ^a  

^a INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Asymmetric; Bridge PCR; Intramolecular homologous recombination; Myostatin

Indexed keywords

ASYMMETRIC; DNA CLONING; HOMOLOGOUS RECOMBINATION; INHERENT LIMITATIONS; ITS EFFICIENCIES; LABOUR-INTENSIVE; MAMMALIAN CELLS; MYOSTATIN; NOVEL STRATEGIES; PRIMER DESIGN; RECOMBINANT PLASMID; REPORTER ASSAY; SINGLE TUBES; SITE-SPECIFIC RECOMBINATION; TRANSCRIPTIONAL ACTIVITY;

CLONING; DNA; DNA SEQUENCES; EFFICIENCY; GENES; TUBES (COMPONENTS);

POLYMERASE CHAIN REACTION;

MYOSTATIN; SINGLE STRANDED DNA; DNA; PRIMER DNA;

ARTICLE; CHIMERA; DNA RECOMBINATION; DNA SEQUENCE; ESCHERICHIA COLI; GENE CASSETTE; GENETIC ENGINEERING; GENETIC TRANSCRIPTION; MAMMAL CELL; MOLECULAR CLONING; NONHUMAN; PLASMID; PROTEIN EXPRESSION; RELIABILITY; VALIDATION STUDY; ANIMAL; CHEMISTRY; GENETICS; HOMOLOGOUS RECOMBINATION; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROMOTER REGION; REPORTER GENE; SWINE;

MAMMALIA; SUIDAE;

ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DNA; DNA PRIMERS; ESCHERICHIA COLI; GENES, REPORTER; GENETIC ENGINEERING; HOMOLOGOUS RECOMBINATION; MOLECULAR SEQUENCE DATA; MYOSTATIN; PLASMIDS; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; SWINE;

EID: 84863459976     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-12-39     Document Type: Article

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