A simple and reproducible method for directed evolution: Combination of random mutation with dITP and DNA fragmentation with endonuclease v

Molecular Biotechnology

Volumn 53, Issue 1, 2013, Pages 49-54

  Wang, Zun ^a   Wang, Hai Yan ^a   Feng, Hong ^a  

^a SICHUAN UNIVERSITY   (China)

Author keywords

dITP; DNA shuffling; Endonuclease V; Mutagenesis; PCR

Indexed keywords

ALKALINE PROTEASE; ALTERNATIVE METHODS; DIRECTED EVOLUTION; DITP; DNA FRAGMENTATION; DNA SHUFFLING; ENDONUCLEASES; ERROR PRONE PCR; KANAMYCINS; MUTATION FREQUENCY; PCR; PCR PRODUCTS; PCR REACTIONS; RANDOM MUTATION;

AMINO ACIDS; GENE ENCODING; MUTAGENESIS;

POLYMERASE CHAIN REACTION;

ALKALINE PROTEINASE; DOUBLE STRANDED DNA; ENDONUCLEASE; ENDONUCLEASE V; UNCLASSIFIED DRUG;

ARTICLE; DNA FRAGMENTATION; GENE MUTATION; GENETIC CODE; MOLECULAR EVOLUTION; MOLECULAR LIBRARY; MUTAGENESIS; NICK END LABELING; POLYMERASE CHAIN REACTION; REPRODUCIBILITY;

CLONING, MOLECULAR; DEOXYRIBONUCLEASE (PYRIMIDINE DIMER); DIRECTED MOLECULAR EVOLUTION; DNA; DNA FRAGMENTATION; DNA SHUFFLING; ESCHERICHIA COLI; GENE LIBRARY; INOSINE TRIPHOSPHATE; KANAMYCIN; MUTAGENESIS; MUTATION; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SEQUENCE ANALYSIS, DNA;

EID: 84871664385     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-012-9516-9     Document Type: Article

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