Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies

BMC Biotechnology

Volumn 9, Issue , 2009, Pages

  Edelheit, Oded ^a,b,c   Hanukoglu, Aaron ^b,c   Hanukoglu, Israel ^a  

^a ARIEL UNIVERSITY   (Israel)

^b TEL AVIV UNIVERSITY   (Israel)

^c WOLFSON MEDICAL CENTER   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

ALTERNATIVE METHODS; ALTERNATIVE PROCEDURES; DNA POLYMERASE; E. COLI; HIGH FIDELITY; OLIGONUCLEOTIDE PRIMERS; PROBABILITY OF SUCCESS; PROTEIN ENGINEERING; PROTEIN STRUCTURES; SITE DIRECTED MUTAGENESIS; SLOWLY COOLED; TANDEM REPEATS;

AMPLIFICATION; BIOCHEMICAL ENGINEERING; DNA; DNA SEQUENCES; ESCHERICHIA COLI; GENES; MUTAGENESIS; NUCLEIC ACIDS; OLIGONUCLEOTIDES; SODIUM; SYNTHESIS (CHEMICAL);

CLONING;

COMPLEMENTARY DNA; DNA POLYMERASE; EPITHELIAL SODIUM CHANNEL; NICOTINAMIDE ADENINE DINUCLEOTIDE; PLASMID DNA; PRIMER DNA;

ANIMAL CELL; ARTICLE; CODON; CONTROLLED STUDY; DNA DENATURATION; DNA METHYLATION; DNA MODIFICATION; DNA SEQUENCE; DNA STRAND; DNA SYNTHESIS; ESCHERICHIA COLI; GENE INSERTION; GENE MUTATION; MOLECULAR CLONING; MOLECULAR INTERACTION; NONHUMAN; OOCYTE; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; TANDEM REPEAT; XENOPUS; HUMAN; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE;

BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; HUMANS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 67650865950     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-9-61     Document Type: Article

References (12)

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