Directed evolution of nucleotide-based libraries using lambda exonuclease

BioTechniques

Volumn 53, Issue 6, 2012, Pages 357-364

  Lim, Bee Nar ^a   Choong, Yee Siew ^a   Ismail, Asma ^a   Glökler, Jörn ^b   Konthur, Zoltán ^c   Lim, Theam Soon ^a  

^a INSTITUTE FOR RESEARCH IN MOLECULAR MEDICINE INFORMM   (Malaysia)

^b ALACRIS THERANOSTICS GMBH   (Germany)

^c MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

Author keywords

Antibody; Diversity; Lambda exonuclease; Mutagenesis; Nucleotide based libraries

Indexed keywords

DOUBLE STRANDED DNA; EXONUCLEASE; LAMBDA EXONUCLEASE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; SINGLE STRANDED DNA; UNCLASSIFIED DRUG;

ANTIBODY LIBRARY; ANTIBODY TITER; CONTROLLED STUDY; DNA FRAGMENTATION; DNA LIBRARY; DNA SEQUENCE; DNA SHUFFLING; HEAVY CHAIN; HUMAN; HUMAN CELL; LIGHT CHAIN; MOLECULAR CLONING; MUTAGENESIS; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; REVIEW;

BACTERIOPHAGE LAMBDA; DIRECTED MOLECULAR EVOLUTION; DNA POLYMERASE I; EXONUCLEASES; FLUORESCENT DYES; GENE LIBRARY; MODELS, GENETIC; VIRAL PROTEINS;

EID: 84871352798     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000113964     Document Type: Review

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