Phosphorothioate-based ligase-independent gene cloning (PLICing): An enzyme-free and sequence-independent cloning method

Analytical Biochemistry

Volumn 406, Issue 2, 2010, Pages 141-146

  Blanusa, Milan ^a   Schenk, Alexander ^a   Sadeghi, Hengameh ^a   Marienhagen, Jan ^b   Schwaneberg, Ulrich ^a,b  

^a JACOBS UNIVERSITY BREMEN   (Germany)

^b RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

Enzyme free cloning; Iodine; Mutant library; Phosphorothioate; Sequence independent cloning

Indexed keywords

CLONE CELLS; EFFICIENCY; ENZYMES; GENES; IODINE; POLYMERASE CHAIN REACTION; PURIFICATION;

CHEMICAL CLEAVAGE; DNA CLEAVAGE; GENE CLONING; MUTANT LIBRARIES; PHOSPHOROTHIOATES; TRANSFORMATION EFFICIENCY;

CLONING;

ALCOHOL; CYTOCHROME P450 BM3; IODINE; PHOSPHOROTHIOIC ACID;

ARTICLE; DNA CLEAVAGE; DNA HYBRIDIZATION; EDFP GENE; GENE; GENE EXPRESSION; GENETIC TRANSFORMATION; MOLECULAR CLONING; PHOSPHOROTHIOATE BASED LIGASE INDEPENDENT GENE CLONING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PRODUCTIVITY; PROM57 GENE; PURIFICATION; RELIABILITY; SOLUTION AND SOLUBILITY;

EID: 77956228537     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.07.011     Document Type: Article

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