A rapid and efficient one-step site-directed deletion, insertion, and substitution mutagenesis protocol

Analytical Biochemistry

Volumn 434, Issue 2, 2013, Pages 254-258

  Wu, Deguang ^a   Guo, Xuewu ^a   Lu, Jun ^a   Sun, Xi ^a   Li, Feng ^a   Chen, Yefu ^a   Xiao, Dongguang ^a  

^a TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Homologous recombination; Mutation frequency; One step ligation; Separate PCR amplification; Site directed mutagenesis

Indexed keywords

ESCHERICHIA COLI; GENES; GENETIC ENGINEERING; MUTAGENESIS; SPACE DIVISION MULTIPLE ACCESS; YEAST;

HOMOLOGOUS RECOMBINATION; MUTATION FREQUENCY; ONE-STEP LIGATION; PCR AMPLIFICATION; SITE DIRECTED MUTAGENESIS;

POLYMERASE CHAIN REACTION;

PROTEINASE ACTIVATED RECEPTOR;

ARTICLE; CLINICAL PROTOCOL; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE DELETION; GENE INSERTION; GENE TARGETING; GENETIC ENGINEERING; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS;

EID: 84873398378     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.11.028     Document Type: Article

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