Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: Application to difficult-to-amplify templates

Applied Microbiology and Biotechnology

Volumn 81, Issue 2, 2008, Pages 387-397

  Sanchis, Joaquin ^a   Fernández, Layla ^a   Carballeira, J Daniel ^a   Drone, Jullien ^a   Gumulya, Yosephine ^a   Höbenreich, Horst ^a   Kahakeaw, Daniel ^a   Kille, Sabrina ^a   Lohmer, Renate ^a   Peyralans, Jérôme J P ^a   Podtetenieff, John ^a   Prasad, Shreenath ^a   Soni, Pankaj ^a   Taglieber, Andreas ^a   Wu, Sheng ^a   Zilly, Felipe E ^a   Reetz, Manfred T ^a  

^a MAX PLANCK INSTITUT FÜR KOHLENFORSCHUNG   (Germany)

Author keywords

Antiprimer; Difficult to amplify templates; Directed evolution; Megaprimer; PCR; Saturation mutagenesis

Indexed keywords

ABS RESINS; BACTERIOLOGY; CATALYSTS; COSMIC RAYS; ENZYMES; LIPASES; MUTAGENESIS; PROTEINS;

ANTIPRIMER; DIFFICULT-TO-AMPLIFY TEMPLATES; DIRECTED EVOLUTION; MEGAPRIMER; PCR; SATURATION MUTAGENESIS;

AMPLIFICATION;

ACID LIPASE; CYTOCHROME P450 BM3; EPOXIDE HYDROLASE; LIPASE B;

BACTERIUM; ENZYME ACTIVITY; EVOLUTION; MUTATION; PLASMID; POLYMERASE CHAIN REACTION; SATURATION;

ARTICLE; ASPERGILLUS NIGER; BACILLUS MEGATERIUM; CANDIDA ANTARCTICA; CONTROLLED STUDY; GENE AMPLIFICATION; GENE SEQUENCE; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA;

ASPERGILLUS NIGER; BACILLUS MEGATERIUM; BACTERIAL PROTEINS; CANDIDA; CYTOCHROME P-450 ENZYME SYSTEM; DIRECTED MOLECULAR EVOLUTION; EPOXIDE HYDROLASES; GENE LIBRARY; LIPASE; MUTAGENESIS; NADPH-FERRIHEMOPROTEIN REDUCTASE; PLASMIDS; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA;

ASPERGILLUS NIGER; BACILLUS MEGATERIUM; CANDIDA ANTARCTICA; PSEUDOMONAS AERUGINOSA;

EID: 55649110680     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1678-9     Document Type: Article

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