USER friendly DNA recombination (USERec): A simple and flexible near homology-independent method for gene library construction

Protein Engineering, Design and Selection

Volumn 23, Issue 1, 2010, Pages 1-8

  Villiers, B R M ^a   Stein, V ^a   Hollfelder, F ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Directed evolution; DNA library; Exon shuffling; Homology independent DNA recombination; USER enzyme

Indexed keywords

DIRECTED EVOLUTION; DNA LIBRARY; DNA RECOMBINATION; EXON SHUFFLING; HOMOLOGY-INDEPENDENT DNA RECOMBINATION;

DNA; ENZYMES; GENES;

DNA SEQUENCES;

DNA FRAGMENT; RESTRICTION ENDONUCLEASE;

ARTICLE; DNA LIBRARY; DNA RECOMBINATION; EXON; GENE AMPLIFICATION; GENE CONSTRUCT; GENE LIBRARY; GENETIC VARIABILITY; MOLECULAR CLONING; MUTAGENESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA; GENE LIBRARY; GENETIC ENGINEERING; MOLECULAR SEQUENCE DATA; MUTAGENESIS; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC;

EID: 73849104591     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzp063     Document Type: Article

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