A Gradient of Sitewise Diversity Promotes Evolutionary Fitness for Binder Discovery in a Three-Helix Bundle Protein Scaffold

Biochemistry

Volumn 56, Issue 11, 2017, Pages 1656-1671

  Woldring, Daniel R ^a   Holec, Patrick V ^a   Stern, Lawrence A ^a   Du, Yang ^b   Hackel, Benjamin J ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

^b STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BINS; LIGANDS; PROTEINS;

COMBINATORIAL LIBRARY; FUNDAMENTAL RESEARCH; HIGH THROUGHPUT; LIBRARY DESIGNS; NOVEL FUNCTIONS; PROTEIN SCAFFOLDS; SEARCHING SEQUENCE; STRUCTURAL COMPUTATIONS;

SCAFFOLDS (BIOLOGY);

AMINO ACID; CYSTEINE; LIGAND; MUTANT PROTEIN; SCAFFOLD PROTEIN; B7 ANTIGEN; CD276 PROTEIN, HUMAN; CYTOCHROME C; G PROTEIN COUPLED RECEPTOR; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; IMMUNOGLOBULIN G; LYSOZYME; MET PROTEIN, HUMAN; PEPTIDE LIBRARY; PROTEIN BINDING; SCATTER FACTOR RECEPTOR; TRANSFERRIN; TUMOR NECROSIS FACTOR RELATED APOPTOSIS INDUCING LIGAND RECEPTOR;

AMINO ACID SEQUENCE; ARTICLE; CIRCULAR DICHROISM; GENE MUTATION; HIGH THROUGHPUT SEQUENCING; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN FUNCTION; PROTEIN STABILITY; PROTEIN STRUCTURE; SACCHAROMYCES CEREVISIAE; SURFACE AREA; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; GENETICS; HUMAN; METABOLISM; MOLECULAR MODEL; MUTATION; PEPTIDE LIBRARY; PROCEDURES; PROTEIN DENATURATION; PROTEIN SECONDARY STRUCTURE;

B7 ANTIGENS; CYTOCHROMES C; DIRECTED MOLECULAR EVOLUTION; GLUCOSEPHOSPHATE DEHYDROGENASE; HUMANS; IMMUNOGLOBULIN G; MODELS, MOLECULAR; MURAMIDASE; MUTATION; PEPTIDE LIBRARY; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN STABILITY; PROTEIN STRUCTURE, SECONDARY; PROTO-ONCOGENE PROTEINS C-MET; RECEPTORS, G-PROTEIN-COUPLED; RECEPTORS, TNF-RELATED APOPTOSIS-INDUCING LIGAND; SACCHAROMYCES CEREVISIAE; TRANSFERRIN;

EID: 85015956694     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/acs.biochem.6b01142     Document Type: Article

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