Fluorescence-activated cell sorting of specific affibody-displaying staphylococci

Applied and Environmental Microbiology

Volumn 69, Issue 9, 2003, Pages 5328-5335

  Wernérus, Henrik ^a   Samuelson, Patrik ^a   Ståhl, Stefan ^a  

^a ALBANOVA UNIVERSITY CENTER   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; CELL CULTURE; FLUORESCENCE; IMMUNOLOGY; MOLECULAR BIOLOGY; PROTEINS; VIRUSES;

LIGANDS;

BACTERIOLOGY;

BACTERIAL PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN; IMMUNOGLOBULIN G; PEPTIDE LIBRARY; PROTEIN A;

BACTERIUM; MICROBIOLOGY;

ARTICLE; BINDING AFFINITY; CELL SURFACE; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; HUMAN; NONHUMAN; PROTEIN DOMAIN; PROTEIN EXPRESSION; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS CARNOSUS;

ANTIBODY AFFINITY; ESCHERICHIA COLI; FLOW CYTOMETRY; HUMANS; IMMUNOGLOBULIN G; PEPTIDE LIBRARY; PLASMIDS; RESTRICTION MAPPING; STAPHYLOCOCCAL PROTEIN A; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS; TRANSFORMATION, GENETIC;

BACTERIA (MICROORGANISMS); HUMAN RESPIRATORY SYNCYTIAL VIRUS; NEGIBACTERIA; PNEUMOVIRUS; POSIBACTERIA; RESPIRATORY SYNCYTIAL VIRUS; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS CARNOSUS;

EID: 0141718631     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.9.5328-5335.2003     Document Type: Article

References (46)

1. Andréoni, C., L. Goetsch, C. Libon, P. Samuelson, T. N. Nguyen, A. Robert, M. Uhlén, H. Binz, and S. Ståhl. 1997. Flow cytometric quantification of surface-displayed recombinant receptors on staphylococci. BioTechniques 23:696-702, 704.
2. Ayora, S., P. E. Lindgren, and F. Götz. 1994. Biochemical properties of a novel metalloprotease from Staphylococcus hyicus subsp. hyicus involved in extracellular lipase processing. J. Bacteriol. 176:3218-3223.
3. Benhar, I. 2001. Biotechnological applications of phage and cell display. Biotechnol. Adv. 19:1-33.
4. Boder, E. T., K. S. Midelfort, and K. D. Wittrup. 2000. Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity. Proc. Natl. Acad. Sci. USA 97:10701-10705.
5. Boder, E. T., and K. D. Wittrup. 2000. Yeast surface display for directed evolution of protein expression, affinity, and stability. Methods Enzymol. 328:430-444.
6. Cano, F., H. Plotnicky-Gilquin, T. N. Nguyen, S. Liljeqvist, P. Samuelson, J. Bonnefoy, S. Ståhl, and A. Robert. 2000. Partial protection to respiratory syncytial virus (RSV) elicited in mice by intranasal immunization using live staphylococci with surface-displayed RSV-peptides. Vaccine 18:2743-2752.
7. Daugherty, P. S., B. L. Iverson, and G. Georgiou. 2000. Flow cytometric screening of cell-based libraries. J. Immunol. Methods 243:211-227.
8. Demleitner, G., and F. Götz. 1994. Evidence for importance of the Staphylococcus hyicus lipase pro-peptide in lipase secretion, stability and activity. FEMS Microbiol. Lett. 121:189-197.
9. Götz, F. 1990. Staphylococcus carnosus: a new host organism for gene cloning and protein production. Soc. Appl. Bacteriol. Symp. Ser. 19:49S-53S.
10. Götz, F., and B. Schumacher. 1987. Improvements of protoplast transformation in Staphylococcus carnosus. FEMS Microbiol. Lett. 40:285-288.
11. Gunneriusson, E., K. Nord, M. Uhlén, and P. Å. Nygren. 1999. Affinity maturation of a TaqDNA polymerase specific affibody by helix shuffling. Protein Eng. 12:873-878.
12. Gunneriusson, E., P. Samuelson, J. Ringdahl, H. Grönlund, P.-Å. Nygren, and S. Ståhl. 1999. Staphylococcal surface display of immunoglobulin A (IgA)- and IgE-specific in vitro-selected binding proteins (affibodies) based on Staphylococcus aureus protein A. Appl. Environ. Microbiol. 65:4134-4140.
13. Gunneriusson, E., P. Samuelson, M. Uhlén, P.-Å. Nygren, and S. Ståhl. 1996. Surface display of a functional single-chain Fv antibody on staphylococci. J. Bacteriol. 178:1341-1346.
14. Hanes, J., and A. Plückthun. 1997. In vitro selection and evolution of functional proteins by using ribosome display. Proc. Natl. Acad. Sci. USA 94:4937-4942.
15. Hansson, M., J. Ringdahl, A. Robert, U. Power, L. Goetsch, T. N. Nguyen, M. Uhlén, S. Ståhl, and P. Å. Nygren. 1999. An in vitro selected binding protein (affibody) shows conformation-dependent recognition of the respiratory syncytial virus (RSV) G protein. Immunotechnology 4:237-252.
16. Hansson, M., S. Ståhl, T. N. Nguyen, T. Bachi, A. Robert, H. Binz, A. Sjölander, and M. Uhlén. 1992. Expression of recombinant proteins on the surface of the coagulase-negative bacterium Staphylococcus xylosus. J. Bacteriol. 174:4239-4245.
17. Holt, L. J., C. Enever, R. M. de Wildt, and I. M. Tomlinson. 2000. The use of recombinant antibodies in proteomics. Curr. Opin. Biotechnol. 11:445-449.
18. Lee, S. Y., J. H. Choi, and Z. Xu. 2003. Microbial cell-surface display. Trends Biotechnol. 21:45-52.
19. Lehtiö, J., T. T. Teeri, and P. Nygren. 2000. Alpha-amylase inhibitors selected from a combinatorial library of a cellulose binding domain scaffold. Proteins 41:316-322.
20. Liebl, W., and F. Götz. 1986. Studies on lipase directed export of Escherichia coli beta-lactamase in Staphylococcus carnosus. Mol. Gen. Genet. 204:166-173.
21. Mazmanian, S. K., G. Liu, H. Ton-That, and O. Schneewind. 1999. Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall. Science 285:760-763.
22. Nilsson, B., T. Moks, B. Jansson, L. Abrahmsén, A. Elmblad, E. Holmgren, C. Henrichson, T. A. Jones, and M. Uhlén. 1987. A synthetic IgG-binding domain based on staphylococcal protein A. Protein Eng. 1:107-113.
23. Nord, K., E. Gunneriusson, J. Ringdahl, S. Ståhl, M. Uhlén, and P. Å. Nygren. 1997. Binding proteins selected from combinatorial libraries of an alpha-helical bacterial receptor domain. Nat. Biotechnol. 15:772-777.
24. Nord, K., J. Nilsson, B. Nilsson, M. Uhlén, and P.-Å. Nygren. 1995. A combinatorial library of an alpha-helical bacterial receptor domain. Protein Eng. 8:601-608.
25. Nord, K., O. Nord, M. Uhlén, B. Kelley, C. Ljungqvist, and P.-Å. Nygren. 2001. Recombinant human factor VIII-specific affinity ligands selected from phage-displayed combinatorial libraries of protein A. Eur. J. Biochem. 268:4269-4277.
26. Nygren, P.-Å., and M. Uhlén. 1997. Scaffolds for engineering novel binding sites in proteins. Curr. Opin. Struct. Biol. 7:463-469.
27. Nygren, P.-Å., M. Eliasson, L. Abrahmsén, M. Uhlén, and E. Palmcrantz. 1988. Analysis and use of the serum albumin binding domains of streptococcal protein G. J. Mol. Recognit. 1:69-74.
28. Olsen, M., B. Iverson, and G. Georgiou. 2000. High-throughput screening of enzyme libraries. Curr. Opin. Biotechnol. 11:331-337.
29. Roberts, R. W. 1999. Totally in vitro protein selection using mRNA-protein fusions and ribosome display. Curr. Opin. Chem. Biol. 3:268-273.
30. Rönnmark, J., H. Grönlund, M. Uhlén, and P.-Å. Nygren. 2002. Human immunoglobulin A (IgA)-specific ligands from combinatorial engineering of protein A. Eur. J. Biochem. 269:2647-2655.
31. Rüther, U. 1982. pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts. Nucleic Acids Res. 10:5765-5772.
32. Samuelson, P., F. Cano, A. Robert, and S. Ståhl. 1999. Engineering of a Staphylococcus carnosus surface display system by substitution or deletion of a Staphylococcus hyicus lipase propeptide. FEMS Microbiol. Lett. 179:131-139.
33. Samuelson, P., E. Gunneriusson, P.-Å. Nygren, and S. Ståhl. 2002. Display of proteins on bacteria. J. Biotechnol. 96:129-154.
34. Samuelson, P., M. Hansson, N. Ahlborg, C. Andréoni, F. Götz, T. Bachi, T. N. Nguyen, H. Binz, M. Uhlén, and S. Ståhl. 1995. Cell surface display of recombinant proteins on Staphylococcus carnosus. J. Bacteriol. 177:1470-1476.
35. Samuelson, P., H. Wernérus, M. Svedberg, and S. Ståhl. 2000. Staphylococcal surface display of metal-binding polyhistidyl peptides. Appl. Environ. Microbiol. 66:1243-1248.
36. Schneewind, O., A. Fowler, and K. F. Faull. 1995. Structure of the cell wall anchor of surface proteins in Staphylococcus aureus. Science 268:103-106.
37. Shusta, E. V., P. D. Holler, M. C. Kieke, D. M. Kranz, and K. D. Wittrup. 2000. Directed evolution of a stable scaffold for T-cell receptor engineering. Nat. Biotechnol. 18:754-759.
38. Skerra, A. 2000. Engineered protein scaffolds for molecular recognition. J. Mol. Recognit. 13:167-187.
39. Smith, G. P., and V. A. Petrenko. 1997. Phage display. Chem. Rev. 97:391-410.
40. Ståhl, S., A. Robert, E. Gunneriusson, H. Wernérus, F. Cano, S. Liljeqvist, M. Hansson, T. N. Nguyen, and P. Samuelson. 2000. Staphylococcal surface display and its applications. Int. J. Med. Microbiol. 290:571-577.
41. Ståhl, S., P. Samuelson, M. Hansson, C. Andréoni, L. Goetsch, C. Libon, S. Liljeqvist, E. Gunneriusson, H. Binz, N. Nguyen, and M. Uhlén. 1997. Development of non-pathogenic staphylococci as vaccine delivery vehicles, p. 62-81. In G. Pozzi and J. Wells (ed.), Gram-positive bacteria: vaccine vehicles for mucosal immunization. Landes Bioscience, Georgetown, Tex.
42. Ton-That, H., S. K. Mazmanian, K. F. Faull, and O. Schneewind. 2000. Anchoring of surface proteins to the cell wall of Staphylococcus aureus. Sortase catalyzed in vitro transpeptidation reaction using LPXTG peptide and NH(2)-Gly(3) substrates. J. Biol. Chem. 275:9876-9881.
43. Wernérus, H., J. Lehtiö, P. Samuelson, and S. Ståhl. 2002. Engineering of staphylococcal surfaces for biotechnological applications. J. Biotechnol. 96:67-78.
44. Wernérus, H., J. Lehtiö, T. Teeri, P.-Å. Nygren, and S. Ståhl. 2001. Generation of metal-binding staphylococci through surface display of combinatorially engineered cellulose-binding domains. Appl. Environ. Microbiol. 67:4678-4684.
45. Wernérus, H., and S. Ståhl. 2002. Vector engineering to improve a staphylococcal surface display system. FEMS Microbiol. Lett. 212:47-54.
46. Wittrup, K. D. 2001. Protein engineering by cell-surface display. Curr. Opin. Biotechnol. 12:395-399.