A hyperthermophilic protein acquires function at the cost of stability

Biochemistry

Volumn 45, Issue 42, 2006, Pages 12673-12679

  Mukaiyama, Atsushi ^a   Haruki, Mitsuru ^b   Ota, Motonori ^c   Koga, Yuichi ^a   Takano, Kazufumi ^a,d   Kanaya, Shigenori ^a  

^a OSAKA UNIVERSITY   (Japan)

^b NIHON UNIVERSITY   (Japan)

^c TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^d JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CONFORMATIONS; ENZYME KINETICS;

ACTIVE SITE RESIDUES; CONFORMATIONAL STABILITY; HYPERTHERMOPHILIC PROTEIN; MUTATIONS;

PROTEINS;

GLUTAMINE; GLYCINE; GUANIDINE HYDROCHLORIDE; MUTANT PROTEIN; PROTEIN D105A; PROTEIN D135A; PROTEIN D7N; PROTEIN E8A; RIBONUCLEASE; UNCLASSIFIED DRUG;

ARTICLE; CIRCULAR DICHROISM; ENZYME ACTIVE SITE; ENZYME CONFORMATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN FUNCTION; PROTEIN STABILITY; STRUCTURE ACTIVITY RELATION; TEMPERATURE DEPENDENCE; THERMOCOCCUS KODAKARAENSIS; THERMODYNAMICS; THERMOPHILIC BACTERIUM;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BINDING SITES; CIRCULAR DICHROISM; CRYSTALLIZATION; ENZYME STABILITY; KINETICS; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; RIBONUCLEASE H, CALF THYMUS; THERMOCOCCUS; THERMODYNAMICS;

THERMOCOCCUS KODAKARENSIS;

EID: 33750349072     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi060907v     Document Type: Article

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