Design, construction, and characterization of a second-generation DARPin library with reduced hydrophobicity

Protein Science

Volumn 22, Issue 9, 2013, Pages 1239-1257

  Seeger, Markus A ^a   Zbinden, Reto ^a   Flütsch, Andreas ^a   Gutte, Petrus G M ^a   Engeler, Sibylle ^a   Roschitzki Voser, Heidi ^a   Grütter, Markus G ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

DARPins; In vitro selection; Library assembly; Protein protein interactions; Ribosome display; Structure based protein engineering; Synthetic binder libraries

Indexed keywords

ANKYRIN; CASPASE 3; CASPASE 7; DESIGNED ANKYRIN REPEAT PROTEIN; ISOLEUCINE; LEUCINE; MEMBRANE PROTEIN; METHIONINE; PHENYLALANINE; TRYPTOPHAN; TYROSINE; UNCLASSIFIED DRUG; VALINE; ACRBP PROTEIN, HUMAN; CARRIER PROTEIN; PEPTIDE; PEPTIDE LIBRARY; PROTEIN BINDING;

AMINO ACID SEQUENCE; ARTICLE; BETA TURN; CELL SELECTION; CRYSTAL STRUCTURE; ENTROPY; ENZYME LINKED IMMUNOSORBENT ASSAY; HYDROPHOBICITY; MOLECULAR LIBRARY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN MODIFICATION; PROTEIN UNFOLDING; RIBOSOME; SURFACE PROPERTY; CHEMICAL PHENOMENA; CHEMICAL STRUCTURE; CHEMISTRY; HUMAN; METABOLISM; PEPTIDE LIBRARY; SYNTHESIS;

ANKYRIN REPEAT; CARRIER PROTEINS; CASPASE 3; CASPASE 7; ENTROPY; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; MODELS, MOLECULAR; PEPTIDE LIBRARY; PEPTIDES; PROTEIN BINDING; SURFACE PROPERTIES; TRYPTOPHAN; TYROSINE;

EID: 84892930656     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.2312     Document Type: Article

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