Affinity recovery of eight HER2-binding affibody variants using an anti-idiotypic affibody molecule as capture ligand

Protein Expression and Purification

Volumn 76, Issue 1, 2011, Pages 127-135

  Wllberg, Helena ^a   Löfdahl, Per Ke ^a   Tschapalda, Kirsten ^a,c   Uhlén, Mathias ^a   Tolmachev, Vladimir ^b   Nygren, Per Ke ^a   Sthl, Stefan ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b UPPSALA UNIVERSITY   (Sweden)

^c BIELEFELD UNIVERSITY   (Germany)

Author keywords

Affibody molecule; Affinity column; Anti idiotypic affibody ligand; Molecular imaging; Protein engineering; Protein fragment complementation assay

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR 2; HER2 PROTEIN, HUMAN; HYBRID PROTEIN; LIGAND;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CHEMISTRY; CIRCULAR DICHROISM; HUMAN; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN SECONDARY STRUCTURE;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, AFFINITY; CIRCULAR DICHROISM; HUMANS; LIGANDS; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STRUCTURE, SECONDARY; RECEPTOR, ERBB-2; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 78650180317     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.10.008     Document Type: Article

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