Recombinant human factor VIII-specific affinity ligands selected from phage-displayed combinatorial libraries of protein A

European Journal of Biochemistry

Volumn 268, Issue 15, 2001, Pages 4269-4277

  Nord, Karin ^a   Nord, Olof ^a   Uhlén, Mathias ^a   Kelley, Brian ^b   Ljungqvist, Charlotta ^c   Nygren, Per Åke ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b PFIZER INC   (United States)

^c Pharmacia Corporation   (Sweden)

Author keywords

Affibody ligand; Affinity chromatography; Affinity maturation; Coagulation factor VIII; Combinatorial phage library

Indexed keywords

BLOOD CLOTTING FACTOR 8; CYSTEINE; LIGAND; MONOCLONAL ANTIBODY; PROTEIN A;

ALPHA HELIX; ARTICLE; BACTERIOPHAGE; BIOSENSOR; CARBOXY TERMINAL SEQUENCE; CHO CELL; CHROMATOGRAPHY; CIRCULAR DICHROISM; COMBINATORIAL LIBRARY; CONTROLLED STUDY; EPITOPE MAPPING; HUMAN; IMMUNOAFFINITY CHROMATOGRAPHY; MONITORING; MUTAGENESIS; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN SECONDARY STRUCTURE; PURIFICATION; STAPHYLOCOCCUS;

AMINO ACID SEQUENCE; ANIMALS; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CIRCULAR DICHROISM; CRICETINAE; CYSTEINE; EPITOPE MAPPING; FACTOR VIII; HUMANS; KINETICS; LIGANDS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PEPTIDE LIBRARY; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; TIME FACTORS;

BACTERIA (MICROORGANISMS); CRICETINAE; CRICETULUS GRISEUS; POSIBACTERIA; STAPHYLOCOCCUS;

EID: 0034832744     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.02344.x     Document Type: Article

References (29)

1. Immunoaffinity chromatography
2. Affinity chromatography
3. Viral inactivation vs. biological activity
4. Affinity chromatography: Production and regulatory considerations
5. Composite affinity sorbents and their cleaning in place
6. Filamentous fusion phage: Novel expression vectors that display cloned antigens on the virion surface
7. Binding proteins selected from combinatorial libraries of an α-helical bacterial receptor domain
8. Ligands selected from combinatorial libraries of protein A for use in affinity capture of apolipoprotein A-1M and taq DNA polymerase
9. Novel forms of B-domain-deleted recombinant factor VIII molecules. Construction and biochemical characterization
10. Immunopurified clotting factor concentrates
11. The purification process for recombinant factor VIII SQ Thromb
12. Purification of factor VIII by monoclonal antibody affinity chromatography
13. pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts
14. R 3 and autogenous negative control by repressor
15. A combinatorial library of an a-helical bacterial receptor domain
16. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support
17. Analysis and use of the serum albumin binding domains of streptococcal protein G
18. A simple and accurate microplate assay for the determination of factor VIII activity
19. Assay of factor VIII: C with a chromogenic substrate
20. A synthetic IgG-binding domain based on staphylococcal protein A
21. Competitive elution of protein A fusion proteins allows specific recovery under mild conditions
22. Comparison of methods for immobilization to carboxymethyl dextran sensor surfaces by analysis of the specific activity of monoclonal antibodies
23. The association of factor VIII with von Willebrand factor
24. An in vitro selected binding protein (affibody) shows conformation-dependent recognition of the respiratory syncytial virus (RSV) G protein
25. Separation processes in biotechnology. Process affinity chromatography
26. Convergent solutions to binding at a protein-protein interface
27. Affinity maturation of a Taq DNA polymerase specific affibody by helix shuffling
28. Isolation of picomolar affinity anti-c-erbB-2 single-chain Fv by molecular evolution of the complementarity determining regions in the center of the antibody binding site