An improved yeast transformation method for the generation of very large human antibody libraries

Protein Engineering, Design and Selection

Volumn 23, Issue 4, 2010, Pages 155-159

  Benatuil, Lorenzo ^a   Perez, Jennifer M ^a   Belk, Jonathan ^b   Hsieh, Chung Ming ^a  

^a ABBOTT LABORATORIES   (United States)

^b Adimab Inc   (United States)

Author keywords

antibody discovery; antibody engineering; antibody libraries; yeast electroporation

Indexed keywords

ANTIBODY ENGINEERING; ANTIBODY LIBRARIES; ANTIBODY LIBRARY; CELL VOLUME; DITHIOTHREITOL; ELECTROPORATION; HIGHLY SENSITIVE; HUMAN ANTIBODIES; LITHIUM ACETATE; MULTIPLE COMPONENTS; TRANSFORMATION EFFICIENCY; TRANSFORMATION METHODS; YEAST DISPLAY;

DNA; GENES; LIBRARIES; LITHIUM; SUGAR (SUCROSE); YEAST;

ANTIBODIES;

CALCIUM CHLORIDE; DITHIOTHREITOL; DNA; LITHIUM ACETATE; MAGNESIUM CHLORIDE; SORBITOL; SUCROSE; ANTIBODY; IMMUNOGLOBULIN F(AB) FRAGMENT; PEPTIDE LIBRARY;

ANTIBODY LIBRARY; ARTICLE; CELL VOLUME; ELECTRIC POTENTIAL; ELECTROPORATION; HUMAN; NONHUMAN; PRIORITY JOURNAL; YEAST; CHEMISTRY; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; IMMUNOLOGY; METABOLISM; METHODOLOGY; PROTEIN ENGINEERING; SACCHAROMYCES CEREVISIAE;

ANTIBODIES; ELECTROPORATION; GENETIC VECTORS; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; PEPTIDE LIBRARY; PROTEIN ENGINEERING; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC;

EID: 77954656041     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzq002     Document Type: Article

References (22)

1. Becker, D.M. and Guarente, L. (1991) Methods Enzymol., 194, 182-187.
2. Chao, G., Lau, W.L., Hackel, B.J., Sazinsky, S.L., Lippow, S.M. and Wittrup, K.D. (2006) Nat. Protoc., 1, 755-768.
3. Feldhaus, M.J., et al. (2003) Nat. Biotechnol., 21, 163-170.
4. Gai, S.A. and Wittrup, K.D. (2007) Curr. Opin. Struct. Biol., 17, 467-473.
5. Gietz, R.D., Schiestl, R.H., Willems, A.R. and Woods, R.A. (1995) Yeast, 11, 355-360.
6. Griffiths, A.D., et al. (1994) EMBO J., 13, 3245-3260.
7. He, M. and Khan, F. (2005) Expert Rev. Proteomics, 2, 421-430.
8. Helmuth, M., Altrock, W., Bockers, T.M., Gundelfinger, E.D. and Kreutz, M.R. (2001) Anal. Biochem., 293, 149-152.
9. Hoet, R.M., et al. (2005) Nat. Biotechnol., 23, 344-348.
10. Hoogenboom, H.R. (2002) Methods Mol. Biol., 178, 1-37.
11. Hoogenboom, H.R. (2005) Nat. Biotechnol., 23, 1105-1116.
12. Lee, H.W., Lee, S.H., Park, K.J., Kim, J.S., Kwon, M.H. and Kim, Y.S. (2006) Biochem. Biophys. Res. Commun., 346, 896-903.
13. Lipovsek, D., Lippow, S.M., Hackel, B.J., Gregson, M.W., Cheng, P., Kapila, A. and Wittrup, K.D. (2007) J. Mol. Biol., 368, 1024-1041.
14. Neumann, E., Kakorin, S., Tsoneva, I., Nikolova, B. and Tomov, T. (1996) Biophys. J., 71, 868-877.
15. Rothe, C., et al. (2008) J. Mol. Biol., 376, 1182-1200.
16. Sblattero, D. and Bradbury, A. (1998) Immunotechnology, 3, 271-278.
17. Sblattero, D. and Bradbury, A. (2000) Nat. Biotechnol., 18, 75-80.
18. Segal, L., Lapidot, M., Solan, Z., Ruppin, E., Pilpel, Y. and Horn, D. (2007) Bioinformatics, 23, i440-i449.
19. Simon, J.R. (1993) Methods Enzymol., 217, 478-483.
20. Suga, M. and Hatakeyama, T. (2003) Curr. Genet., 43, 206-211.
21. Thompson, J.R., Register, E., Curotto, J., Kurtz, M. and Kelly, R. (1998) Yeast, 14, 565-571.
22. Weaver, J.C., Harrison, G.I., Bliss, J.G., Mourant, J.R. and Powell, K.T. (1988) FEBS Lett., 229, 30-34.