Functional genomic approaches targeting the Wnt signaling network

Current Drug Targets

Volumn 10, Issue 7, 2009, Pages 620-631

  DasGupta, Ramanuj ^a  

^a NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

HTS; Network; RNAi; Signaling; Wingless; Wnt

Indexed keywords

AXIN; BETA CATENIN; CALCIUM CALMODULIN DEPENDENT PROTEIN KINASE II; CASEIN KINASE IALPHA; CYCLIN D1; DOUBLE STRANDED RNA; GLYCOGEN SYNTHASE KINASE 3BETA; GUANOSINE TRIPHOSPHATASE; HIGH MOBILITY GROUP PROTEIN; LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN 5; LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN 6; LYMPHOID ENHANCER FACTOR 1; MITOGEN ACTIVATED PROTEIN KINASE 1; MYC PROTEIN; PHOSPHOLIPASE C; RETINOIC ACID RECEPTOR; RHO KINASE; STRESS ACTIVATED PROTEIN KINASE; T CELL FACTOR PROTEIN; TRANSFORMING GROWTH FACTOR BETA; TRANSFORMING GROWTH FACTOR BETA ACTIVATED KINASE 1; UVOMORULIN; WNT1 PROTEIN; SMALL INTERFERING RNA; WNT PROTEIN;

CARCINOGENESIS; CELL ADHESION; CELL DEATH; CELL GROWTH; CELL PROLIFERATION; CELL STRUCTURE; COLORECTAL CANCER; DROSOPHILA; FUNCTIONAL GENOMICS; GENE EXPRESSION; GENE TECHNOLOGY; GENETIC CODE; GENETIC SCREENING; GENETIC TRANSCRIPTION; HUMAN; MAMMAL CELL; NONHUMAN; PROTEIN DEGRADATION; PROTEIN PROTEIN INTERACTION; REVIEW; RNA INTERFERENCE; SIGNAL TRANSDUCTION; ANIMAL; GENE EXPRESSION REGULATION; GENETICS; GENOMICS; METABOLISM; METHODOLOGY; NEOPLASM; PATHOLOGY;

ANIMALS; GENE EXPRESSION REGULATION; GENOMICS; HUMANS; NEOPLASMS; RNA INTERFERENCE; RNA, DOUBLE-STRANDED; RNA, SMALL INTERFERING; SIGNAL TRANSDUCTION; WNT PROTEINS;

EID: 70249114836     PISSN: 13894501     EISSN: None     Source Type: Journal    
DOI: 10.2174/138945009788680455     Document Type: Review

References (163)

1. St Johnston D. The art and design of genetic screens: Drosophila melanogaster. Nat Rev Genet 2002; 3(3): 176-188 (Pubitemid 34506586)
2. Jorgensen EM, Mango SE. The art and design of genetic screens: caenorhabditis elegans. Nat Rev Genet 2002; 3(5): 356-369 (Pubitemid 34506717)
3. Gunsalus KC, Ge H, Schetter AJ, Goldberg DS, Han JD, Hao T, et al. Predictive models of molecular machines involved in Caenorhabditis elegans early embryogenesis. Nature 2005; 436(7052): 861-865 (Pubitemid 41160646)
4. Boxem M, Maliga Z, Klitgord N, Li N, Lemmens I, Mana M et al. A protein domain-based interactome network for C. elegans early embryogenesis. Cell 2008; 134(3): 534-545
5. Tewari M, Hu PJ, Ahn JS, Ayivi-Guedehoussou N, Vidalain PO, Li S, et al. Systematic interactome mapping and genetic perturbation analysis of a C. elegans TGF-beta signaling network. Mol Cell 2004; 13(4): 469-482. (Pubitemid 38299376)
6. De Toni F, Racaud-Sultan C, Chicanne G, Mas VM, Cariven C, Mesange F, et al. A crosstalk between the Wnt and the adhesiondependent signaling pathways governs the chemosensitivity of acute myeloid leukemia. Oncogene 2006; 25(22): 3113-3122 (Pubitemid 43881553)
7. Hasson P, Egoz N, Winkler C, Volohonsky G, Jia S, Dinur T, et al. EGFR signaling attenuates Groucho-dependent repression to antagonize Notch transcriptional output. Nat Genet 2005; 37(1): 101-105 (Pubitemid 40070947)
8. Letamendia A, Labbe E, Attisano L. Transcriptional regulation by Smads: crosstalk between the TGF-beta and Wnt pathways. J Bone Joint Surg Am 2001; 83-A Suppl 1(Pt 1): S31-9. (Pubitemid 32246598)
9. Lum L, Yao S, Mozer B, Rovescalli A, Von Kessler D, Nirenberg M, et al. Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells. Science (New York, NY 2003; 299(5615): 2039-2045 (Pubitemid 36383726)
10. Musgrove EA. Wnt signalling via the epidermal growth factor receptor: a role in breast cancer? Breast Cancer Res 2004; 6(2): 65-68
11. von Bubnoff A, Cho KW. Intracellular BMP signaling regulation in vertebrates: pathway or network? Dev Biol 2001; 239(1): 1-14.
12. Nusse R. Wnts and Hedgehogs: lipid-modified proteins and similarities in signaling mechanisms at the cell surface. Development (Cambridge, England) 2003; 130(22): 5297-5305. (Pubitemid 37483999)
13. Miller JR, Hocking AM, Brown JD, Moon RT. Mechanism and function of signal transduction by the Wnt/beta-catenin and Wnt/Ca2+ pathways. Oncogene 1999; 18(55): 7860-7872
14. Polakis P. Wnt signaling and cancer. Genes Develop 2000; 14(15): 1837-1851
15. Wodarz A, Nusse R. Mechanisms of Wnt signaling in development. Annu Rev Cell Dev Biol 1998; 1459-1488
16. Ciruna B, Jenny A, Lee D, Mlodzik M, Schier AF. Planar cell polarity signalling couples cell division and morphogenesis during neurulation. Nature 2006; 439(7073): 220-224 (Pubitemid 43083145)
17. Grove EA, Tole S, Limon J, Yip L, Ragsdale CW. The hem of the embryonic cerebral cortex is defined by the expression of multiple Wnt genes and is compromised in Gli3-deficient mice. Development (Cambridge, England) 1998; 125(12): 2315-2325 (Pubitemid 28336391)
18. Jiang R, Bush JO, Lidral AC. Development of the upper lip: morphogenetic and molecular mechanisms. Dev Dyn 2006; 235(5): 1152-1166 (Pubitemid 43843904)
19. Kokubu C, Heinzmann U, Kokubu T, Sakai N, Kubota T, Kawai M, et al. Skeletal defects in ringelschwanz mutant mice reveal that Lrp6 is required for proper somitogenesis and osteogenesis. Development (Cambridge, England) 2004; 131(21): 5469-5480 (Pubitemid 39562189)
20. Shu W, Jiang YQ, Lu MM, Morrisey EE. Wnt7b regulates mesenchymal proliferation and vascular development in the lung. Development (Cambridge, England) 2002; 129(20): 4831-4842 (Pubitemid 35277208)
21. Staal FJ, Clevers H. Tcf/Lef transcription factors during T-cell development: unique and overlapping functions. Hematol J 2000; 1(1): 3-6.
22. Kaykas A, Yang-Snyder J, Heroux M, Shah KV, Bouvier M, Moon RT. Mutant Frizzled 4 associated with vitreoretinopathy traps wildtype Frizzled in the endoplasmic reticulum by oligomerization. Nat Cell Biol 2004; 6(1): 52-58 (Pubitemid 38425730)
23. Robitaille J, MacDonald ML, Kaykas A, Sheldahl LC, Zeisler J, Dube MP, et al. Mutant frizzled-4 disrupts retinal angiogenesis in familial exudative vitreoretinopathy. Nat Genet 2002; 32(2): 326-330
24. Miyoshi K, Hennighausen L. Beta-catenin: a transforming actor on many stages. Breast Cancer Res 2003; 5(2): 63-68 (Pubitemid 36348098)
25. Miyoshi K, Rosner A, Nozawa M, Byrd C, Morgan F, Landesman- Bollag E, et al. Activation of different Wnt/beta-catenin signaling components in mammary epithelium induces transdifferentiation and the formation of pilar tumors. Oncogene 2002; 21(36): 5548-5556 (Pubitemid 34983544)
26. Moon RT, Kohn AD, De Ferrari GV, Kaykas A. WNT and betacatenin signalling: diseases and therapies. Nat Rev Genet 2004; 5(9): 691-701. (Pubitemid 39150127)
27. Nusse R. WNT targets. Repression and activation. Trends Genet 1999; 15(1): 1-3.
28. Peifer M, Orsulic S, Pai LM, Loureiro J. A model system for cell adhesion and signal transduction in Drosophila. Dev Suppl 1993; 163-176 (Pubitemid 24139757)
29. McCrea PD, Turck CW, Gumbiner B. A homolog of the armadillo protein in Drosophila (plakoglobin) associated with E-cadherin. Science (New York, NY 1991; 254(5036): 1359-1361 (Pubitemid 21917467)
30. Willert K, Logan CY, Arora A, Fish M, Nusse R. A Drosophila Axin homolog, Daxin, inhibits Wnt signaling. Development (Cambridge, England) 1999; 126(18): 4165-4173 (Pubitemid 29481573)
31. Ikeda S, Kishida S, Yamamoto H, Murai H, Koyama S, Kikuchi A. Axin, a negative regulator of the Wnt signaling pathway, forms a complex with GSK-3beta and beta-catenin and promotes GSK- 3beta-dependent phosphorylation of beta-catenin. EMBO J 1998; 17(5): 1371-1384 (Pubitemid 28105516)
32. Liu C, Kato Y, Zhang Z, Do VM, Yankner BA, He X. beta-Trcp couples beta-catenin phosphorylation-degradation and regulates Xenopus axis formation. Proc Nat Acad Sci USA 1999; 96(11): 6273-6278 (Pubitemid 29256655)
33. Latres E, Chiaur DS, Pagano M. The human F box protein beta- Trcp associates with the Cul1/Skp1 complex and regulates the stability of beta-catenin. Oncogene 1999; 18(4): 849-854 (Pubitemid 29086261)
34. Tamai K, Semenov M, Kato Y, Spokony R, Liu C, Katsuyama Y, et al. LDL-receptor-related proteins in Wnt signal transduction. Nature 2000; 407(6803): 530-535 (Pubitemid 30746709)
35. He X, Semenov M, Tamai K, Zeng X. LDL receptor-related proteins 5 and 6 in Wnt/beta-catenin signaling: arrows point the way. Development (Cambridge, England) 2004; 131(8): 1663-1677 (Pubitemid 38559836)
36. Zeng X, Tamai K, Doble B, Li S, Huang H, Habas R, et al. A dualkinase mechanism for Wnt co-receptor phosphorylation and activation. Nature 2005; 438(7069): 873-877 (Pubitemid 41753071)
37. Zeng X, Huang H, Tamai K, Zhang X, Harada Y, Yokota C, et al. Initiation of Wnt signaling: control of Wnt coreceptor Lrp6 phosphorylation/activation via frizzled, dishevelled and axin functions. Development (Cambridge, England) 2008; 135(2): 367-375 (Pubitemid 351228428)
38. Daniels DL, Weis WI. Beta-catenin directly displaces Groucho/ TLE repressors from Tcf/Lef in Wnt-mediated transcription activation. Nat Struct Mol Biol 2005; 12(4): 364-371
39. Valenta T, Lukas J, Korinek V. HMG box transcription factor TCF-4's interaction with CtBP1 controls the expression of the Wnt target Axin2/Conductin in human embryonic kidney cells. Nucleic Acids Res 2003; 31(9): 2369-2380 (Pubitemid 37442105)
40. Stadeli R, Basler K. Dissecting nuclear Wingless signalling: recruitment of the transcriptional co-activator Pygopus by a chain of adaptor proteins. Mech Develop 2005; 122(11): 1171-1182 (Pubitemid 41476322)
41. Hoffmans R, Basler K. Identification and in vivo role of the Armadillo-Legless interaction. Development (Cambridge, England) 2004; 131(17): 4393-4400
42. Townsley FM, Cliffe A, Bienz M. Pygopus and Legless target Armadillo/beta-catenin to the nucleus to enable its transcriptional co-activator function. Nat Cell Biol 2004; 6(7): 626-633 (Pubitemid 38960920)
43. Kramps T, Peter O, Brunner E, Nellen D, Froesch B, Chatterjee S, et al. Wnt/wingless signaling requires BCL9/legless-mediated recruitment of pygopus to the nuclear beta-catenin-TCF complex. Cell 2002; 109(1): 47-60. (Pubitemid 34327516)
44. Logan CY, Nusse R. The Wnt signaling pathway in development and disease. Annu Rev Cell Dev Biol 2004; 20781-20810
45. Nusse R. Wnt signaling in disease and in development. Cell research 2005; 15(1): 28-32.
46. Katoh M. WNT/PCP signaling pathway and human cancer (review). Oncol Rep 2005; 14(6): 1583-1588
47. Fanto M, McNeill H. Planar polarity from flies to vertebrates. J Cell Sci 2004; 117(Pt 4): 527-533
48. Kohn AD, Moon RT. Wnt and calcium signaling: beta-cateninindependent pathways. Cell Calcium 2005; 38(3-4): 439-446 (Pubitemid 41283282)
49. Andersson ER, Prakash N, Cajanek L, Minina E, Bryja V, Bryjova L et al. Wnt5a regulates ventral midbrain morphogenesis and the development of A9-A10 dopaminergic cells in vivo. PLoS ONE 2008; 3(10): e3517.
50. Habas R, Dawid IB, He X. Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation. Genes Dev 2003; 17(2): 295-309. (Pubitemid 36105900)
51. Habas R, He X. Activation of Rho and Rac by Wnt/frizzled signaling. Meth Enzymol 2006; 406500-406511
52. Nakaya MA, Habas R, Biris K, Dunty WC, Jr., Kato Y, He X, et al. Identification and comparative expression analyses of Daam genes in mouse and Xenopus. Gene Expr Patterns 2004; 5(1): 97-105. (Pubitemid 39529805)
53. Park TJ, Gray RS, Sato A, Habas R, Wallingford JB. Subcellular localization and signaling properties of dishevelled in developing vertebrate embryos. Curr Biol 2005; 15(11): 1039-1044 (Pubitemid 40772152)
54. Moriguchi T, Kawachi K, Kamakura S, Masuyama N, Yamanaka H, Matsumoto K et al. Distinct domains of mouse dishevelled are responsible for the c-Jun N-terminal kinase/stress-activated protein kinase activation and the axis formation in vertebrates. J Biol Chem 1999; 274(43): 30957-30962 (Pubitemid 129576846)
55. Yamanaka H, Moriguchi T, Masuyama N, Kusakabe M, Hanafusa H, Takada R, et al. JNK functions in the non-canonical Wnt pathway to regulate convergent extension movements in vertebrates. EMBO reports 2002; 3(1): 69-75. (Pubitemid 34144818)
56. Veeman MT, Slusarski DC, Kaykas A, Louie SH, Moon RT. Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements. Curr Biol 2003; 13(8): 680-685 (Pubitemid 36453318)
57. Wang HY, Malbon CC. Wnt signaling, Ca2+, and cyclic GMP: visualizing Frizzled functions. Science 2003; 300(5625): 1529-1530
58. Wang HY, Malbon CC. Wnt-frizzled signaling to G-proteincoupled effectors. Cell Mol Life Sci 2004; 61(1): 69-75.
59. Kuhl M. Non-canonical Wnt signaling in Xenopus: regulation of axis formation and gastrulation. Semin Cell Develop Biol 2002; 13(3): 243-249 (Pubitemid 35304529)
60. Kuhl M, Pandur P. Measuring CamKII activity in Xenopus embryos as a read-out for non-canonical Wnt signaling. Methods Mol Biol (Clifton, NJ 2008; 468173-468186
61. Kuhl M, Sheldahl LC, Malbon CC, Moon RT. Ca(2+)/calmodulindependent protein kinase II is stimulated by Wnt and Frizzled homologs and promotes ventral cell fates in Xenopus. J Biol Chem 2000; 275(17): 12701-12711
62. Behrens J. Cross-regulation of the Wnt signalling pathway: a role of MAP kinases. J Cell Sci 2000; 113 ( Pt 6)911-919 (Pubitemid 30179471)
63. Ishitani T, Kishida S, Hyodo-Miura J, Ueno N, Yasuda J, Waterman M, et al. The TAK1-NLK mitogen-activated protein kinase cascade functions in the Wnt-5a/Ca(2+) pathway to antagonize Wnt/beta-catenin signaling. Mol Cell Biol 2003; 23(1): 131-139
64. Ishitani T, Ninomiya-Tsuji J, Nagai S, Nishita M, Meneghini M, Barker N, et al. The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF. Nature 1999; 399(6738): 798-802. (Pubitemid 29293177)
65. Meneghini MD, Ishitani T, Carter JC, Hisamoto N, Ninomiya-Tsuji J, Thorpe CJ, et al. MAP kinase and Wnt pathways converge to downregulate an HMG-domain repressor in Caenorhabditis elegans. Nature 1999; 399(6738): 793-797.
66. Smit L, Baas A, Kuipers J, Korswagen H, van de Wetering M, Clevers H. Wnt activates the Tak1/Nemo-like kinase pathway. J Biol Chem 2004; 279(17): 17232-17240. (Pubitemid 38560481)
67. Arsura M, Panta GR, Bilyeu JD, Cavin LG, Sovak MA, Oliver AA et al. Transient activation of NF-kappaB through a TAK1/IKK kinase pathway by TGF-beta1 inhibits AP-1/SMAD signaling and apoptosis: implications in liver tumor formation. Oncogene 2003; 22(3): 412-425. (Pubitemid 36194337)
68. Kaminska B, Wesolowska A, Danilkiewicz M. TGF beta signalling and its role in tumour pathogenesis. Acta Biochim Pol 2005; 52(2): 329-337. (Pubitemid 41133768)
69. Matsumoto-Ida M, Takimoto Y, Aoyama T, Akao M, Takeda T, Kita T. Activation of TGF-beta1-TAK1-p38 MAPK pathway in spared cardiomyocytes is involved in left ventricular remodeling after myocardial infarction in rats. Am J Physiol Heart Circ Physiol 2006; 290(2): H709-715.
70. Hayward P, Brennan K, Sanders P, Balayo T, DasGupta R, Perrimon N, et al. Notch modulates Wnt signalling by associating with Armadillo/beta-catenin and regulating its transcriptional activity. Development (Cambridge, England) 2005; 132(8): 1819-1830. (Pubitemid 40691772)
71. Helms W, Lee H, Ammerman M, Parks AL, Muskavitch MA, Yedvobnick B. Engineered truncations in the Drosophila mastermind protein disrupt Notch pathway function. Dev Biol 1999; 215(2): 358-374.
72. Yedvobnick B, Helms W, Barrett B. Identification of chromosomal deficiencies that modify Drosophila mastermind mutant phenotypes. Genesis 2001; 30(4): 250-258. (Pubitemid 32816508)
73. Alves-Guerra MC, Ronchini C, Capobianco AJ. Mastermind-like 1 Is a specific coactivator of beta-catenin transcription activation and is essential for colon carcinoma cell survival. Cancer Res 2007; 67(18): 8690-8698. (Pubitemid 47437444)
74. Mulholland DJ, Dedhar S, Coetzee GA, Nelson CC. Interaction of nuclear receptors with the Wnt/beta-catenin/Tcf signaling axis: Wnt you like to know? Endocr Rev 2005; 26(7): 898-915.
75. Easwaran V, Pishvaian M, Salimuddin, Byers S. Cross-regulation of beta-catenin-LEF/TCF and retinoid signaling pathways. Curr Biol 1999; 9(23): 1415-1418.
76. Kennell JA, MacDougald OA. Wnt signaling inhibits adipogenesis through beta-catenin-dependent and -independent mechanisms. The Journal of biological chemistry 2005; 280(25): 24004-24010. (Pubitemid 40884888)
77. Prestwich TC, Macdougald OA. Wnt/beta-catenin signaling in adipogenesis and metabolism. Curr Opin Cell Biol 2007; 19(6): 612-617.
78. Jansson EA, Are A, Greicius G, Kuo IC, Kelly D, Arulampalam V et al. The Wnt/beta-catenin signaling pathway targets PPARgamma activity in colon cancer cells. Proc Nat Acad Sci USA 2005; 102(5): 1460-1465. (Pubitemid 40209192)
79. Crosetto N, Bienko M, Dikic I. Ubiquitin hubs in oncogenic networks. Mol Cancer Res 2006; 4(12): 899-904.
80. McDonald SA, Preston SL, Lovell MJ, Wright NA, Jankowski JA. Mechanisms of disease: from stem cells to colorectal cancer. Nat Clin Pract Gastroenterol Hepatol 2006; 3(5): 267-274. (Pubitemid 43675023)
81. van Es JH, Barker N, Clevers H. You Wnt some, you lose some: oncogenes in the Wnt signaling pathway. Curr Opin Genet Dev 2003; 13(1): 28-33. (Pubitemid 36143924)
82. van Noort M, Meeldijk J, van der Zee R, Destree O, Clevers H. Wnt signaling controls the phosphorylation status of beta-catenin. J Biol Chem 2002; 277(20): 17901-17905. (Pubitemid 34967600)
83. Winer IS, Bommer GT, Gonik N, Fearon ER. Lysine residues Lys- 19 and Lys-49 of beta-catenin regulate its levels and function in T cell factor transcriptional activation and neoplastic transformation. J Biol Chem 2006; 281(36): 26181-26187. (Pubitemid 44401825)
84. Segditsas S, Tomlinson I. Colorectal cancer and genetic alterations in the Wnt pathway. Oncogene 2006; 25(57): 7531-7537. (Pubitemid 44885752)
85. Clevers H. Wnt breakers in colon cancer. Cancer Cell 2004; 5(1): 5-6.
86. Taniguchi K, Roberts LR, Aderca IN, Dong X, Qian C, Murphy LM, et al. Mutational spectrum of beta-catenin, AXIN1, and AXIN2 in hepatocellular carcinomas and hepatoblastomas. Oncogene 2002; 21(31): 4863-4871.
87. Satoh S, Daigo Y, Furukawa Y, Kato T, Miwa N, Nishiwaki T, et al. AXIN1 mutations in hepatocellular carcinomas, and growth suppression in cancer cells by virus-mediated transfer of AXIN1. Nat Genet 2000; 24(3): 245-250. (Pubitemid 30132191)
88. Chan EF, Gat U, McNiff JM, Fuchs E. A common human skin tumour is caused by activating mutations in beta-catenin. Nat Genet 1999; 21(4): 410-413. (Pubitemid 29159579)
89. DasGupta R, Fuchs E. Multiple roles for activated LEF/TCF transcription complexes during hair follicle development and differentiation. Development (Cambridge, England) 1999; 126(20): 4557-4568.
90. Gat U, DasGupta R, Degenstein L, Fuchs E. De Novo hair follicle morphogenesis and hair tumors in mice expressing a truncated beta-catenin in skin. Cell 1998; 95(5): 605-614. (Pubitemid 28544122)
91. Barker N, van Es JH, Kuipers J, Kujala P, van den Born M, Cozijnsen M et al. Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature 2007; 449(7165): 1003-1007. (Pubitemid 350014593)
92. Charafe-Jauffret E, Monville F, Ginestier C, Dontu G, Birnbaum D, Wicha MS. Cancer stem cells in breast: current opinion and future challenges. Pathobiology 2008; 75(2): 75-84. (Pubitemid 351820725)
93. Chiba T, Zheng YW, Kita K, Yokosuka O, Saisho H, Onodera M, et al. Enhanced self-renewal capability in hepatic stem/progenitor cells drives cancer initiation. Gastroenterology 2007; 133(3): 937-950. (Pubitemid 47374130)
94. Congdon KL, Voermans C, Ferguson EC, DiMascio LN, Uqoezwa M, Zhao C et al. Activation of Wnt signaling in hematopoietic regeneration. Stem cells (Dayton, Ohio) 2008; 26(5): 1202-1210.
95. Fodde R, Brabletz T. Wnt/beta-catenin signaling in cancer stemness and malignant behavior. Curr Opin Cell Biol 2007; 19(2): 150-158. (Pubitemid 46386404)
96. Kang S, Bennett CN, Gerin I, Rapp LA, Hankenson KD, Macdougald OA. Wnt signaling stimulates osteoblastogenesis of mesenchymal precursors by suppressing CCAAT/enhancer-binding protein alpha and peroxisome proliferator-activated receptor gamma. J Biol Chem 2007; 282(19): 14515-14524. (Pubitemid 47100486)
97. Lobo NA, Shimono Y, Qian D, Clarke MF. The biology of cancer stem cells. Annu Rev Cell Dev Biol 2007; 23675-23699
98. Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, et al. A role for Wnt signalling in self-renewal of haematopoietic stem cells. Nature 2003; 423(6938): 409-414. (Pubitemid 36626987)
99. Sinner D, Rankin S, Lee M, Zorn AM. Sox17 and beta-catenin cooperate to regulate the transcription of endodermal genes. Development (Cambridge, England) 2004; 131(13): 3069-3080. (Pubitemid 39077432)
100. Zhang C, Basta T, Jensen ED, Klymkowsky MW. The betacatenin/ VegT-regulated early zygotic gene Xnr5 is a direct target of SOX3 regulation. Development (Cambridge, England) 2003; 130(23): 5609-5624. (Pubitemid 37540838)
101. Banziger C, Soldini D, Schutt C, Zipperlen P, Hausmann G, Basler K. Wntless, a conserved membrane protein dedicated to the secretion of Wnt proteins from signaling cells. Cell 2006; 125(3): 509-522.
102. Bartscherer K, Pelte N, Ingelfinger D, Boutros M. Secretion of Wnt ligands requires Evi, a conserved transmembrane protein. Cell 2006; 125(3): 523-533.
103. DasGupta R, Kaykas A, Moon RT, Perrimon N. Functional genomic analysis of the Wnt-wingless signaling pathway. Science (NY 2005; 308(5723): 826-833. (Pubitemid 40629258)
104. Dasgupta R, Perrimon N. Using RNAi to catch Drosophila genes in a web of interactions: insights into cancer research. Oncogene 2004; 23(51): 8359-8365. (Pubitemid 39540717)
105. Hausmann G, Banziger C, Basler K. Helping Wingless take flight: how WNT proteins are secreted. Nat Rev Mol Cell Biol 2007; 8(4): 331-336. (Pubitemid 46474657)
106. Yang L, Lin C, Liu ZR. P68 RNA Helicase Mediates PDGFInduced Epithelial Mesenchymal Transition by Displacing Axin from beta-Catenin. Cell 2006; 127(1): 139-155. (Pubitemid 44466634)
107. Adams MD, Celniker SE, Holt RA, Evans CA, Gocayne JD, Amanatides PG, et al. The genome sequence of Drosophila melanogaster. Science (New York, NY 2000; 287(5461): 2185-2195.
108. Rubin GM, Yandell MD, Wortman JR, Gabor Miklos GL, Nelson CR, Hariharan IK, et al. Comparative genomics of the eukaryotes. Science (New York, NY 2000; 287(5461): 2204-2215.
109. Venter JC, Adams MD, Sutton GG, Kerlavage AR, Smith HO, Hunkapiller M. Shotgun sequencing of the human genome. Science (New York, NY 1998; 280(5369): 1540-1542.
110. Hild M, Beckmann B, Haas SA, Koch B, Solovyev V, Busold C et al. An integrated gene annotation and transcriptional profiling approach towards the full gene content of the Drosophila genome. Genome Biol 2003; 5(1): R3.
111. Oliver B, Leblanc B. How many genes in a genome? Genome Biol 2003; 5(1): 204.
112. Sharp PA. RNAi and double-strand RNA. Genes Develop 1999; 13(2): 139-141.
113. Hutvagner G, Simard MJ, Mello CC, Zamore PD. Sequencespecific inhibition of small RNA function. PLoS Biol 2004; 2(4): E98. (Pubitemid 39234504)
114. Mello CC, Conte D, Jr. Revealing the world of RNA interference. Nature 2004; 431(7006): 338-342.
115. Montgomery MK. RNA interference: unraveling a mystery. Nat Struct Mol Biol 2006; 13(12): 1039-1041.
116. Sen CK, Roy S. miRNA: licensed to kill the messenger. DNA and Cell Biol 2007; 26(4): 193-194.
117. Tabara H, Grishok A, Mello CC. RNAi in C. elegans: soaking in the genome sequence. Science (New York, NY 1998; 282(5388): 430-431.
118. Clemens JC, Worby CA, Simonson-Leff N, Muda M, Maehama T, Hemmings BA, et al. Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways. Proc Nat Acad Sc USA 2000; 97(12): 6499-6503. (Pubitemid 30412742)
119. Zamore PD, Haley B. Ribo-gnome: the big world of small RNAs. Science (New York, NY 2005; 309(5740): 1519-1524.
120. Bridge AJ, Pebernard S, Ducraux A, Nicoulaz AL, Iggo R. Induction of an interferon response by RNAi vectors in mammalian cells. Nat Genet 2003; 34(3): 263-264. (Pubitemid 36792856)
121. Scacheri PC, Rozenblatt-Rosen O, Caplen NJ, Wolfsberg TG, Umayam L, Lee JC, et al. Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells. Proc Nat Acad Sci USA 2004; 101(7): 1892-1897. (Pubitemid 38228987)
122. Sledz CA, Holko M, de Veer MJ, Silverman RH, Williams BR. Activation of the interferon system by short-interfering RNAs. Nat Cell Biol 2003; 5(9): 834-839. (Pubitemid 37087172)
123. Yang S, Tutton S, Pierce E, Yoon K. Specific double-stranded RNA interference in undifferentiated mouse embryonic stem cells. Mol Cell Biol 2001; 21(22): 7807-7816. (Pubitemid 32988789)
124. Baeg GH, Zhou R, Perrimon N. Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila. Genes Develop 2005; 19(16): 1861-1870. (Pubitemid 41186987)
125. Friedman A, Perrimon N. A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Nature 2006; 444(7116): 230-234. (Pubitemid 44722065)
126. Nybakken K, Vokes SA, Lin TY, McMahon AP, Perrimon N. A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. Nat Genet 2005; 37(12): 1323-1332. (Pubitemid 41722187)
127. Kiger AA, Baum B, Jones S, Jones MR, Coulson A, Echeverri C, et al. A functional genomic analysis of cell morphology using RNA interference. J Biol 2003; 2(4): 27.
128. Bakal C, Linding R, Llense F, Heffern E, Martin-Blanco E, Pawson T, et al. Phosphorylation networks regulating JNK activity in diverse genetic backgrounds. Science (New York, NY 2008; 322(5900): 453-456.
129. Boutros M, Kiger AA, Armknecht S, Kerr K, Hild M, Koch B, et al. Genome-wide RNAi analysis of growth and viability in Drosophila cells. Science (NY 2004; 303(5659): 832-835.
130. Eggert US, Kiger AA, Richter C, Perlman ZE, Perrimon N, Mitchison TJ, et al. Parallel chemical genetic and genome-wide RNAi screens identify cytokinesis inhibitors and targets. PLoS Biol 2004; 2(12): e379. (Pubitemid 40280520)
131. Friedman A, Perrimon N. Genome-wide high-throughput screens in functional genomics. Curr Opin Genet Dev 2004; 14(5): 470-476. (Pubitemid 39365986)
132. Berns K, Hijmans EM, Mullenders J, Brummelkamp TR, Velds A, Heimerikx M, et al. A large-scale RNAi screen in human cells identifies new components of the p53 pathway. Nature 2004; 428(6981): 431-437. (Pubitemid 38419689)
133. Grimm D, Pandey K, Kay MA. Adeno-associated virus vectors for short hairpin RNA expression. Meth Enzymol 2005; 392381-392405
134. Milhavet O, Gary DS, Mattson MP. RNA interference in biology and medicine. Pharmacol Rev 2003; 55(4): 629-648.
135. Paddison PJ, Silva JM, Conklin DS, Schlabach M, Li M, Aruleba S, et al. A resource for large-scale RNA-interference-based screens in mammals. Nature 2004; 428(6981): 427-431. (Pubitemid 38419688)
136. Zheng L, Liu J, Batalov S, Zhou D, Orth A, Ding S, et al. An approach to genomewide screens of expressed small interfering RNAs in mammalian cells. Proc Nat Acad Sci USA 2004; 101(1): 135-140. (Pubitemid 38084216)
137. Kennerdell JR, Carthew RW. Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway. Cell 1998; 95(7): 1017-1026.
138. Kennerdell JR, Carthew RW. Heritable gene silencing in Drosophila using double-stranded RNA. Nat Biotechnol 2000; 18(8): 896-898.
139. Bale AE. Hedgehog signaling and human disease. Ann Rev Genom Hum Genet 2002; 347-365
140. Belvin MP, Anderson KV. A conserved signaling pathway: the Drosophila toll-dorsal pathway. Annu Rev Cell Dev Biol 1996; 12393-12416
141. Evans CJ, Hartenstein V, Banerjee U. Thicker than blood: conserved mechanisms in Drosophila and vertebrate hematopoiesis. Develop Cell 2003; 5(5): 673-690. (Pubitemid 37362212)
142. Pandur P, Maurus D, Kuhl M. Increasingly complex: new players enter the Wnt signaling network. Bioessays 2002; 24(10): 881-884. (Pubitemid 35180843)
143. Wajant H, Scheurich P. Analogies between Drosophila and mammalian TRAF pathways. Progr Mol SubcellBiol 2004; 3447-3472
144. Gwack Y, Sharma S, Nardone J, Tanasa B, Iuga A, Srikanth S, et al. A genome-wide Drosophila RNAi screen identifies DYRKfamily kinases as regulators of NFAT. Nature 2006; 441(7093): 646-650. (Pubitemid 43927297)
145. Adams MD, Sekelsky JJ. From sequence to phenotype: reverse genetics in Drosophila melanogaster. Nat Rev Genet 2002; 3(3): 189-198.
146. Nagy A, Perrimon N, Sandmeyer S, Plasterk R. Tailoring the genome: the power of genetic approaches. Nat Genet 2003; 33 Suppl276-284.
147. Echeverri CJ, Perrimon N. High-throughput RNAi screening in cultured cells: a user's guide. Nat Rev Genet 2006; 7(5): 373-384.
148. Korinek V, Barker N, Willert K, Molenaar M, Roose J, Wagenaar G, et al. Two members of the Tcf family implicated in Wnt/betacatenin signaling during embryogenesis in the mouse. Mol Cell Biol 1998; 18(3): 1248-1256. (Pubitemid 28100888)
149. He X. Unwinding a Path to Nuclear beta-Catenin. Cell 2006; 127(1): 40-42.
150. Kim JH, Kim B, Cai L, Choi HJ, Ohgi KA, Tran C, et al. Transcriptional regulation of a metastasis suppressor gene by Tip60 and beta-catenin complexes. Nature 2005; 434(7035): 921-926. (Pubitemid 40559007)
151. Sierra J, Yoshida T, Joazeiro CA, Jones KA. The APC tumor suppressor counteracts beta-catenin activation and H3K4 methylation at Wnt target genes. Genes Develop 2006; 20(5): 586-600. (Pubitemid 43345324)
152. Kennell JA, Gerin I, MacDougald OA, Cadigan KM. The microRNA miR-8 is a conserved negative regulator of Wnt signaling. Proc Nat Acad Sci USA 2008; 105(40): 15417-15422
153. Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004; 116(2): 281-297
154. Zamore PD, Tuschl T, Sharp PA, Bartel DP. RNAi: doublestranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell 2000; 101(1): 25-33.
155. Echeverri CJ, Beachy PA, Baum B, Boutros M, Buchholz F, Chanda SK, et al. Minimizing the risk of reporting false positives in large-scale RNAi screens. Nat Methods 2006; 3(10): 777-779 (Pubitemid 44445821)
156. Saxena S, Jonsson ZO, Dutta A. Small RNAs with imperfect match to endogenous mRNA repress translation. Implications for offtarget activity of small inhibitory RNA in mammalian cells. J Biol Chem 2003; 278(45): 44312-44319 (Pubitemid 37377177)
157. Birmingham A, Anderson EM, Reynolds A, Ilsley-Tyree D, Leake D, Fedorov Y et al. 3' UTR seed matches, but not overall identity, are associated with RNAi off-targets. Nat Methods 2006; 3(3): 199-204.
158. Off-Target Effects: Disturbing the Silence of RNA interference (RNAi)
159. Kulkarni MM, Booker M, Silver SJ, Friedman A, Hong P, Perrimon N, et al. Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays. Nat Methods 2006; 3(10): 833-838 (Pubitemid 44445831)
160. Ma Y, Creanga A, Lum L, Beachy PA. Prevalence of off-target effects in Drosophila RNA interference screens. Nature 2006; 443(7109): 359-363 (Pubitemid 44435157)
161. Carmell MA, Hannon GJ. RNase III enzymes and the initiation of gene silencing. Nat Struct Mol Biol 2004; 11(3): 214-218
162. Major MB, Roberts BS, Berndt JD, Marine S, Anastas J, Chung N et al. New regulators of Wnt/beta-catenin signaling revealed by integrative molecular screening. Sci Signal 2008; 1(45): ra12.
163. Dietzl G, Chen D, Schnorrer F, Su KC, Barinova Y, Fellner M, et al. A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Nature 2007; 448(7150): 151-156 (Pubitemid 47067374)