Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells

Science

Volumn 303, Issue 5659, 2004, Pages 832-835

  Boutros, Michael ^a,e   Kiger, Amy A ^a   Armknecht, Susan ^a,b   Kerr, Kim ^a,b   Hild, Marc ^c   Koch, Britta ^c   Haas, Stefan A ^d   Paro, Renato ^c   Perrimon, Norbert ^a,b  

^a HARVARD MEDICAL SCHOOL   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^c UNIVERSITY OF HEIDELBERG   (Germany)

^d MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

^e GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; CELLS; GENES; GROWTH KINETICS; MUTAGENESIS; TUMORS;

HOMOLOGY; MUTANTS;

RNA;

DOUBLE STRANDED RNA;

GENOME;

ALLELISM; AML1 GENE; ANIMAL CELL; ARTICLE; CELL COUNT; CELL CULTURE; CELL GROWTH; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; DROSOPHILA; GENE; GENE FUNCTION; GENE IDENTIFICATION; GENE SEQUENCE; GENETIC ANALYSIS; GENOMICS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RNA INTERFERENCE; SEQUENCE HOMOLOGY;

ANIMALS; APOPTOSIS; CELL CYCLE; CELL SURVIVAL; CELLS, CULTURED; COMPUTATIONAL BIOLOGY; CORE BINDING FACTOR ALPHA 2 SUBUNIT; DNA-BINDING PROTEINS; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; GENES, ESSENTIAL; GENES, INSECT; GENOME; HUMANS; INHIBITOR OF APOPTOSIS PROTEINS; PHENOTYPE; PROTEOME; PROTO-ONCOGENE PROTEINS; REPRODUCIBILITY OF RESULTS; RNA INTERFERENCE; RNA, DOUBLE-STRANDED; SEQUENCE HOMOLOGY; TRANSCRIPTION FACTORS;

ANIMALIA; MAMMALIA;

EID: 0842309871     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1091266     Document Type: Article

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24. We thank T. Mitchison and the Institute of Chemistry and Cell Biology for advice on cell-based screens and usage of equipment; L. Hrdlicka and S. Hagar for excellent technical support; R. Steen for use of equipment; L. Kockel, B. Gelbart, and D. Emmert for constructive discussions; and G. Rubin, T. Ingolia, P. Leder, T. Mitchison, A. McMahon, L. Perkins, R. Tanis, M. Vincent, and B. Ward for support at various stages of this project. Supported by HHMI, as well as a gift from M. Crowinshield and additional support from Harvard Medical School. Work in R.P. laboratory was supported by the German Human Genome Project (DHGP). M.B. was supported by an Emmy-Noether grant from the Deutsche Forschungsgemeinschaft. A.K. was supported by The Jane Coffin Childs Memorial Fund for Medical Research.