From sequence to phenotype: Reverse genetics in Drosophila melanogaster

Nature Reviews Genetics

Volumn 3, Issue 3, 2002, Pages 189-198

  Adams, Melissa D ^a   Sekelsky, Jeff J ^a  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED RNA;

CHEMICAL MUTAGENESIS; DNA TRANSPOSITION; DROSOPHILA MELANOGASTER; GENE DELETION; GENE DISRUPTION; GENE INSERTION; GENE SEQUENCE; GENE SILENCING; GENE TECHNOLOGY; HYBRID GENE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; REVIEW; TRANSPOSON;

ANIMALS; DNA REPAIR; DROSOPHILA MELANOGASTER; GENE EXPRESSION; GENE TARGETING; GENES, INSECT; GENETIC SCREENING; MODELS, GENETIC; MUTAGENESIS, INSERTIONAL; PHENOTYPE; RECOMBINATION, GENETIC;

DROSOPHILA MELANOGASTER; INSERTION SEQUENCES; MELANOGASTER; TRANSPOSONS;

EID: 0036250571     PISSN: 14710056     EISSN: None     Source Type: Journal    
DOI: 10.1038/nrg752     Document Type: Review

References (75)

1. The art and design of genetic screens: Drosophila melanogaster
2. The genome sequence of Drosophila melanogaster
3. Method for feeding ethyl-methane sulfonate (EMS) to Drosophila males Drosoph
4. Mutational specificity of ethyl methanesulfonate in excision-repair-proficient and -deficient strains of Drosophila melanogaster
5. Targeted recovery of mutations in Drosophila
6. A pre-Columbian Y chromosome-specific transition and its implications for human evolutionary history
7. Kpn which revert the prune/Killer of prune lethal interaction affect conserved residues that are involved in nucleoside diphosphate kinase substrate binding and catalysis
8. The mutability of the minute loci of Drosophila melanogaster with ethyl methanesulfonate
9. The Berkeley Drosophila Genome Project Gene Disruption Project: Single P-element insertions mutating 25% of vital Drosophila genes
10. Searching for pattern and mutation in the Drosophila genome with P-lacZ vector
11. Gene disruptions using P transposable elements: An integral component of the Drosophila genome project
12. Genetic transformation of Drosophila with transposable element vectors
13. Systematic gain-of-function genetics in Drosophila
14. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
15. Dual-tagging gene trap of novel genes in Drosophila melanogaster
16. A P element containing suppressor of Hairy-wing binding regions has novel properties for mutagenesis in Drosophila melanogaster
17. The Su(Hw) protein insulates the expression of the Drosophila melanogaster white gene from chromosomal position-effects
18. On the molecular mechanism of gypsy-induced mutations at the yellow locus of Drosophila melanogaster
19. Efficient and dispersed local P element transposition from Drosophila females
20. Targeted gene mutations in Drosophila
21. 'Site-selected' transposon mutagenesis of Drosophila
22. Genetic analysis of a Drosophila microtubule-associated protein
23. An inverse PCR screen for the detection of P element insertions in cloned genomic intervals in Drosophila melanogaster
24. Frequent imprecise excision among reversions of a P element-caused lethal mutation in Drosophila
25. Dysgenesis-induced instability of rosy locus transformation in Drosophila melanogaster: Analysis of excision events and the selective recovery of control element deletions
26. Drosophila P-element transposase is a novel site-specific endonuclease
27. High-frequency P element loss in Drosophila is homolog dependent
28. Protected P-element termini suggest a role for inverted repeat-binding protein in transposase-induced gap repair in Drosophila melanogaster
29. In situ dissection of the Fab- 7 region of the bithorax complex into a chromatin domain boundary and a Polycomb-response element
30. The Drosophila p 38 MAPK pathway is required during oogenesis for egg asymmetric development
31. Drosophila E 22 promotes the conversion from genomic DNA replication to gene amplification in ovarian follicle cells
32. Targeted gene replacement in Drosophila via P element-induced gap repair
33. Gene targeting of a plasmid-borne sequence to a double-strand DNA break in Drosophila melanogaster
34. In vivo gap repair in Drosophila: A one-way street with many destinations
35. Genetic instability in Drosophila melanogaster: P-element mutagenesis by gene conversion
36. Targeted transposition at the vestigial locus of Drosophila melanogaster
37. Enhancer-trap targeting at the Broad-Complex locus of Drosophila melanogaster
38. P-element-induced recombination in Drosophila melanogaster: Hybrid element insertion
39. Flanking duplications and deletions associated with P-induced male recombination in Drosophila
40. Mapping of Drosophila mutations using site-specific male recombination
41. mei-W68 in Drosophila melanogaster encodes a Spo 11 homolog: Evidence that the mechanism for initiating meiotic recombination is conserved
42. Gene targeting by homologous recombination in Drosophila
43. A targeted gene knockout in Drosophila
44. RAD1 and RAD10, but not other excision repair genes, are required for double-strand break-induced recombination in Saccharomyces cerevisiae
45. The effect of chromosomal position on the expression of the Drosophila xanthine dehrydrogenase gene
46. Transformation of white locus DNA in Drosophila: Dosage compensation, zeste interaction, and position effects
47. Developmentally regulated expression of Drosophila chorion genes introduced at diverse chromosomal positions
48. Post-transcriptional gene silencing by double-stranded RNA
49. Evidence that processed small dsRNAs may mediate sequence-specific mRNA degradation during RNAi in Drosophila embryos
50. RNAi: Double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals
51. RNA interference is mediated by 21- and 22-nucleotide RNAs
52. An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans
53. An extensive class of small RNAs in Caenorhabditis elegans
54. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
55. Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways
56. Essential roles of Drosophila inner centromere protein (INCENP) and aurora B in histone H3 phosphoryation, metaphase chromosome alignment, kinetochore disjunction, and chromosome segregation
57. Drosophila aurora B kinase is required for histone H 3 phosphorydation and condensin recruitment during chromosome condensation and to organize the central spindle during cytoknesis
58. dsRNA-mediated gene silencing in cultured Drosophila cells: A tissue culture model for the analysis of RNA interference
59. Targeted disruption of gene function in Drosophila by RNA interference (RNA-i): A role for nautilus in embryonic somatic muscle formation
60. Heritable gene silencing in Drosophila using double-stranded RNA
61. Inducible expression of double-stranded RNA directs specific genetic interference in Drosophila
62. Efficient and heritable functional knock-out of an adult phenotype in Drosophila using a GAL4-driven hairpin RNA incorporating a heterologous spacer
63. Comparison of physical and genetic properties of palindromic DNA sequences
64. Temperature-dependent gene silencing by an expressed inverted repeat in Drosophila
65. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis
66. Functional genomic analysis of C. elegans chromosome I by systematic RNA interference
67. Functional genomic analysis of cell division in C elegans using RNAi of genes on chromosome III
68. RNAi analysis of genes expressed in the ovary of Caenorhabditis elegans
69. Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi
70. Cis-acting DNA sequence requirements for P-element transposition
71. P element transposition in vitro proceeds by a cut-and-paste mechanism and uses GTP as a cofactor
72. hobo enhancer trapping mutagenesis in Drosophila reveals an insertion specificity different from P elements
73. A versatile vector set for animal transgenesis
74. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells