Measuring CamKII activity in xenopus embryos as a read-out for non-canonical Wnt signaling

Methods in Molecular Biology

Volumn 468, Issue , 2008, Pages 173-186

  Kühl, Michael ^a   Pandur, Petra ^a  

^a UNIVERSITY OF ULM   (Germany)

Author keywords

Ca2+ calmodulin dependent kinase II; Calcium; CamKII; Enzyme activity assay; Non canonical signaling; Wnts; Xenopus

Indexed keywords

CALCIUM CALMODULIN DEPENDENT PROTEIN KINASE II; WNT PROTEIN; XENOPUS PROTEIN;

ANIMAL; ARTICLE; BIOASSAY; METABOLISM; METHODOLOGY; PHYSIOLOGY; PRENATAL DEVELOPMENT; SIGNAL TRANSDUCTION; XENOPUS LAEVIS;

ANIMALS; BIOLOGICAL ASSAY; CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE TYPE 2; SIGNAL TRANSDUCTION; WNT PROTEINS; XENOPUS LAEVIS; XENOPUS PROTEINS;

EID: 58749107556     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-249-6_13     Document Type: Article

References (21)

1. Slusarski, D. C., Corees, V. G., and Moon, R. T. (1997a) Interaction of Wnt and a Frizzled homologue triggers G-proteinlinked phosphatidylinositol signaling. Nature 390, 410-413.
2. 2+ signaling by Wnt-5A. Dev. Biol. 182, 114-120.
3. Eisenberg, L. M. and Eisenberg, C. A. (2002) Onset of a cardiac phenotype in the early embryo. Cardiovascular molecular morphogenesis: myofibrillogenesis. New York: Springer Verlag.
4. Kühl, M. (2002) Non-canonical Wnt signaling in Xenopus: regulation of axis formation and gastrulation. Semin. Cell. Dev. Biol. 13, 243-249.
5. Westfall, T. A., Brimeyer, R., Twedt, J., Gladon, J., Olberding, A., Furutani-Seiki, M., and Slusarski, D. C (2003) Wnt-5/pipetail functions in vertebrate axis formation as a negative regulator of Wnt/beta-catenin activity/ Cell. Biol. 162, 889-898.
6. Kremenevskaja, N., von Wasielewski, R., Rao, A. S., Schofl, C., Andersson, T., and Brabant, G. (2005) Wnt-5a has tumor suppressor activity in thyroid carcinoma. Oncogene 24, 2144-2154.
7. Säfholm, A., Leandersson, K., Dejmek, J., Nielsen, C K., Villoutreix, B. O., and Andersson, T. (2006) A formylated hexapeptide ligand mimics the ability of Wnt-5a to impair migration of human breast epithelial cells. J. Biol. Chem. 281, 2740-2749.
8. 2+-induced NFAT activity is counteracted by Wnt-5a/Yes-Cdc42Casein kinase la signaling in human mammary epithelial cells. Mol. Cell. Biol. 26, 6024-6036.
9. 2+ pathway. J Biol Chem. 281, 30990-31001.
10. Sheldahl, L. C., Park, M., Malbon, C. C., and Moon, R. T. (1999) Protein kinase C. is differentially stimulated by Wnt and Frizzled homologs in a G-protein-dependent manner. Curr. Biol. 9, 695-698.
11. Winklbauer, R, Medina, A., Swain, R.K., and Steinbeisser, H. (2001) Frizzled-7 signalling controls tissue separation during Xenopus gastrulation. Nature413, 856-860.
12. Kinoshita, N., Iioka, H., Miyakoshi, A., and Ueno, N. (2003) PKC delta is essential for Dishevelled function in a non-canonical Wnt pathway that regulates Xenopus convergent extension movements. Genes Dev. 17, 1663-1676.
13. Tu, X., Joeng, K. S., Nakayama, K. I., Nakayama, K., Rajagopal, J., Carroll, T. J., Mc Mahon, A. P., and Long, F. (2007) Non-canonical Wnt signaling through G-protein-linked PKC delta activation promotes bone formation. Dev. Cell 12, 113-127.
14. Saneyoshi, T., Kume, S., Amasaki, Y., and Mikoshiba, K. (2002) The Wnt/calcium pathway activates NF-AT and promotes ventral cell fate in Xenopus embryos. Nature 417, 295-299.
15. 2+/Calmodulin-dependent protein kinase II is stimulated by Wnt and Frizzled homologs and promotes ventral cell fates in Xenopus. J. Biol. Chem. 275, 12701-12711.
16. Ishitani, T., Kishida, S., Hyodo-Miura, J., Ueno, N., Yasuda, J., Waterman, M., Shibuya, H., Moon, R. T., Ninomiya-Tsuji, J., and Matsumoto, K. (2003) The TAKI-NLK mitogen-activated protein kinase cascade functions in the Wnt-5a/Ca(2+) pathway to antagonize Wnt-beta catenin signaling. Mol. Cell. Biol. 23, 131-139.
17. Topol, L., Jiang, X., Choi, H., GarrettBeal, L., Carolan, P. J., and Yang, Y. (2003) Wnt-5a inhibits the canonical Wnt pathway by promoting GSK-3-independent betacatenin degradation. J. Cell. Biol. 162, 899-908.
18. Ouko, L., Ziegler, T. R., Gu, L. H., Eisenberg, L. M., and Yang, V W (2004) Wnt11 signaling promotes proliferation, transformation, and migration of IEC6 intestinal epithelial cells. J. Biol. Chem. 279, 26707-26715.
19. Garriock, R. J. and Krieg, P. A. (2007) Wnt11-R signaling regulates a calcium sensitive EMT event essential for dorsal fin development of Xenopus. Dev. Biol. 304, 127-140.
20. Nieuwkoop, P. D. and Faber, J. (1967) Normaltabelle von Xenopus laevis. Elsevier North-Holland Biomedical Press, Amsterdam.
21. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of bacteriophage T4. Nature 227, 680-685.