The art and design of genetic screens: Caenorhabditis elegans

Nature Reviews Genetics

Volumn 3, Issue 5, 2002, Pages 356-369

  Jorgensen, Erik M ^a   Mango, Susan E ^b  

^a UNIVERSITY OF UTAH   (United States)

^b UNIVERSITY OF UTAH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CAENORHABDITIS ELEGANS; GENE IDENTIFICATION; GENE ISOLATION; GENE MUTATION; GENETIC SCREENING; GENOME ANALYSIS; MOLECULAR CLONING; MOLECULAR EVOLUTION; MOLECULAR GENETICS; MOLECULAR MODEL; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; REVIEW; SIGNAL TRANSDUCTION; TECHNIQUE;

ANIMALS; CAENORHABDITIS ELEGANS; GENES, LETHAL; MUTATION; SELECTION (GENETICS); SIGNAL TRANSDUCTION;

CAENORHABDITIS; CAENORHABDITIS ELEGANS;

EID: 0036254584     PISSN: 14710056     EISSN: None     Source Type: Journal    
DOI: 10.1038/nrg794     Document Type: Review

References (119)

1. The natural history of Caenorhabditis elegans research
2. The genetics of Caenorhabditis elegans
3. Control of type-D GABAergic neuron differentiation by C. Elegans UNC-30 homeodomain protein
4. The unc-5, unc-6, and unc-40 genes guide circumferential migration of pioneer axons and mesodermal cells on the epidermis in C. Elegans
5. UNC-6, a laminin-related protein, guides cell and pioneer axon migrations in C. Elegans
6. Abnormal cell lineages in mutants of the nematode Caenorhabditis elegans
7. Identification and characterization of 22 genes that affect the vulval cell lineages of Caenorhabditis elegans
8. Pattern formation during C. Elegans vulval induction
9. let-60, a gene that specifies cell fates during C. Elegans vulval induction, encodes a ras protein
10. Caenorhabditis elegans ras gene let-60 acts as a switch in the pathway of vulval induction
11. Role of a raf proto-oncogene during Caenorhabditis elegans vulval development
12. A MAP kinase homolog, mpk-1, is involved in ras-mediated induction of vulval cell fates in Caenorhabditis elegans
13. Suppression of activated LET-60 ras protein defines a role of Caenorhabditis elegans sur-1 MAP kinase in vulval differentiation
14. The ksr-1 gene encodes a novel protein kinase involved in ras-mediated signaling in C. Elegans
15. The Caenorhabditis elegans gene mek-2 is required for vulval induction and encodes a protein similar to the protein kinase MEK
16. sur-2, a novel gene, functions late in the let-60 ras-mediated signaling pathway during Caenorhabditis elegans vulval induction
17. Han elegans ksr-1 gene encodes a novel Raf-related kinase involved in Ras-mediated signal transduction
18. Genetics of RAS signaling
19. Genes involved in two Caenorhabditis elegans cell-signaling pathways
20. More sex·determination mutants of Caenorhabditis elegans
21. xol-1: A gene that controls the male modes of both sex determination and X chromosome dosage compensation in C elegans
22. The Caenorhabditis elegans gene sdc-2 controls sex determination and dosage compensation in XX animals
23. The Caenorhabditis elegans unc-17 gene: A putative vesicular acetylcholine transporter
24. The C. Elegans unc-104 gene encodes a putative kinesin heavy chain-like protein
25. The unc-18 gene encodes a novel protein affecting the kinetics of acetylcholine metabolism in the nematode Caenorhabditis elegans
26. A phorbol ester/diacylglycerol-binding protein encoded by the unc-13 gene of Caenorhabditis elegans
27. Synaptic function is impaired but not eliminated in C. Elegans mutants lacking synaptotagmin
28. UNC-11, a Caenorhabditis elegans AP180 homologue, regulates the size and protein composition of synaptic vesicles
29. Mutations in synaptojanin disrupt synaptic vesicle recycling
30. s protein induces neural degeneration
31. s neurodegeneration in Caenorhabditis elegans
32. Dominant unc-37 mutations suppress the movement defect of a homeodomain mutation in unc-4, a neural specificity gene in C. Elegans
33. UNC-4/UNC-37-dependent repression of motor neuron-specific genes controls synaptic choice in Caenorhabditis elegans
34. Independent domains of the SDC-3 protein control sex determination and dosage compensation in C. Elegans
35. The VAB-1 Eph receptor tyrosine kinase functions in neural and epithelial morphogenesis in C. Elegans
36. The ephrin VAB-2/EFN-1 functions in neuronal signaling to regulate epidermal morphogenesis in C. Elegans
37. MEC-2 regulates C. Elegans DEG/ENaC channels needed for mechanosensation
38. MRT-2 checkpoint protein is required for germline immortality and telomere replication in C. Elegans
39. Mutations affecting programmed cell deaths in the nematode Caenorhabditis elegans
40. Genetic control of programmed cell death in the nematode C. Elegans
41. The molecular mechanism of programmed cell death in C. Elegans
42. Caenorhabditis elegans gene ced-9 protects cells from programmed cell death
43. C. Elegans cell survival gene ced-9 encodes a functional homolog of the mammalian proto-oncogene bcl-2
44. Green fluorescent protein as a marker for gene expression
45. Visualization of synaptic specializations in live C. Elegans with synaptic vesicle protein-GFP fusions
46. rpm-1, a conserved neuronal gene that regulates targeting and synaptogenesis in C. Elegans
47. Regulation of presynaptic terminal organization by C. Elegans RPM-1, a putative guanine nucleotide exchanger with a RING-H 2 finger domain
48. The SAD-1 kinase regulates presynaptic vesicle clustering and axon termination
49. The conserved immunoglobulin superfamily member SAX-3/Robo directs multiple aspects of axon guidance in C. Elegans
50. Specification of thermosensory neuron fate in C. Elegans requires ttx-1, a homolog of otd/Otx
51. The RING finger/B-box factor TAM-1 and a retinoblastoma-like protein LIN-35 modulate context-dependent gene silencing in Caenorhabditis elegans
52. Receptor-mediated endocytosis in the Caenorhabditis elegans oocyte
53. Regulation of endocytosis by CUP-5, the Caenorhabditis elegans mucolipin-1 homolog
54. Genetic analysis of endocytosis in Caenorhabditis elegans: Coelomocyte uptake defective mutants
55. Identification of heterochronic mutants in Caenorhabditis elegans. Temporal misexpression of a collagen: Green fluorescent protein fusion gene
56. Lateral signaling mediated by axon contact and calcium entry regulates asymmetric odorant receptor expression in C. Elegans
57. A cell that dies during wild-type C. Elegans development can function as a neuron in a ced-3 mutant
58. The genetics of feeding in Caenorhabditis elegans
59. A mutation in the C. Elegans EXP-2 potassium channel that alters feeding behavior
60. The unc-22(IV) region of Caenorhabditis elegans: Genetic analysis of lethal mutations
61. Genetic analysis of a major segment (LGV(left)) of the genome of Caenorhabditis elegans
62. What does a worm want with 20,000 genes?
63. Crossover suppressors and balanced recessive lethals in Caenorhabditis elegans
64. The genetic analysis of a reciprocal translocation, eT1 (III; V), in Caenorhabditis elegans
65. ELT-1, a GATA-like transcription factor, is required for epidermal cell fates in Caenorhabditis elegans embryos
66. The glp-1 locus and cellular interactions in early C. Elegans embryos
67. Identification of genes required for cytoplasmic localization of early C. Elegans embryos
68. skn-1, a maternally expressed gene required to specify the fate of ventral blastomeres in the early C. Elegans embryo
69. A genetic screen for tempereture-sensitive cell-division mutants of Caenorhabditis elegans
70. Embryonic tissue differentiation in Caenorhabditis elegans requires dif-1, a gene homologous to mitochondrial solute carriers
71. Patterning the C. Elegans embryo: Moving beyond the cell lineage
72. Embryonic polarity: Protein stability in asymmetric cell division
73. Dissection of cell division processes in the one cell stage Caenorhabditis elegans embryo by mutational analysis
74. Cellular interactions in early C. Elegans embryos
75. aph-2 encodes a novel extracellular protein required for GLP-1-mediated signaling
76. APH-1 is a multipass membrane protein essential for the Notch signaling pathway in Caenorhabditis elegans embryos
77. Rasl and a putative guanine nucleotide exchange factor perform crucial steps in signaling by the sevenless protein tyrosine kinase
78. Genetic interactions affecting touch sensitivity in Caenorhabditis elegans
79. mRNA surveillance mitigates genetic dominance in Caenorhabditis elegans
80. Rules of nonallelic noncomplementation at the synapse in Caenorhabditis elegans
81. Genome sequence of the nematode C. Elegans: A platform for investigating biology
82. Changing styles in C. Elegans genetics
83. Functional genomic analysis of C. Elegans chromosome I by systematic RNA interference
84. Functional genomic analysis of cell division in C. Elegans using RNAi of genes on chromosome III
85. Thinking about genetic redundancy
86. end-1 encodes an apparent GATA factor that specifies the endoderm precursor in Caenorhabditis elegans embryos
87. Two homologous regulatory genes, lin-12 and glp-1, have overlapping functions
88. lag-1, a gene required for lin-12 and glp-1 signaling in Caenorhabditis elegans, is homologous to human CBF1 and Drosophila Su(H)
89. lag-2 may encode a signaling ligand for the GLP-1 and LIN-12 receptors of C. Elegans
90. Sequence of C. Elegans, lag-2 reveals a cell-signalling domain shared with Delta and Serrate of Drosophila
91. The multivulva phenotype of certain C. Elegans mutants results from defects in two functionally-redundant pathways
92. The synthetic multivulval genes of C. Elegans: Functional redundancy, Ras-antagonism, and cell fate determination
93. The mec-8 gene of C. Elegans, encodes a protein with two RNA recognition motifs and regulates alternative splicing of unc. 52 transcripts
94. Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae
95. Functional overlap between the mec-8 gene and five sym genes in Caenorhabditis elegans
96. fzr-1 and lin-35/Rb function redundantly to control cell proliferation in C. Elegans as revealed by a nonbiased synthetic screen
97. Natural variation in a nouropeptide Y receptor homolog modifies social behavior and food response in C. Elegans
98. Post-embryonic cell lineages of the nematode Caenorhabditis elegans
99. The embryonic cell lineage of the nematode Caenorhabditis elegans
100. Toward a physical map of the genome of the nematode C. Elegans
101. Integrative transformation of Caenorhabditis elegans
102. Single nucleotide polymorphisms in wild isolates of Caenorhabditis elegans
103. Mobilization of a Drosophila transposon in the Caenorhabditis elegans germ line
104. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
105. The rest is silence
106. Stop making nonsense: The C. Elegans smg genes
107. RNAi as a tool for understanding germline development in Caenorhabditis elegans: Uses and cautions
108. Broeks., A., Van Meurs, J., Groenen, J. T. & Plasterk, R. H. Target·selected gene inactivation in Caenorhabditis elegans by using a frozen transposon insertion mutant bank
109. Reverse genetics by chemical mutagenesis in Caenorhabditis elegans
110. High-throughput isolation of Caenorhabditis elegans deletion mutants
111. Reverse genetics by chemical mutagenesis in Caenorhabditis elegans
112. The Caenorhabditis elegans, unc-32 gene encodes alternative forms of a vacuolar ATPase a subunit