Quo vadis? The challenges of recombinant protein folding and secretion in Pichia pastoris

Applied Microbiology and Biotechnology

Volumn 99, Issue 7, 2015, Pages 2925-2938

  Puxbaum, Verena ^a,b   Mattanovich, Diethard ^a,b   Gasser, Brigitte ^a,b  

^a AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

^b UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Folding; Glycosylation; Pichia pastoris; Recombinant protein; Secretion; Yeast

Indexed keywords

ESTERIFICATION; GLYCOSYLATION; PHYSIOLOGY; PRODUCTION PLATFORMS; PROTEIN FOLDING; PROTEINS; STRAIN; YEAST;

FOLDING; INTRACELLULAR TRAFFICKING; PICHIA PASTORIS; PRODUCTION PROCESS; PRODUCTION STRAIN; PROTEOLYTIC DEGRADATION; SECRETION; STRAIN IMPROVEMENT;

RECOMBINANT PROTEINS;

FUNGAL PROTEIN; RECOMBINANT PROTEIN; SECRETORY PROTEIN; POLYSACCHARIDE;

CELL MIGRATION; ENDOPLASMIC RETICULUM STRESS; HUMAN; KOMAGATAELLA PASTORIS; NONHUMAN; PROTEIN DEGRADATION; PROTEIN FOLDING; PROTEIN GLYCOSYLATION; PROTEIN SECRETION; PROTEIN SYNTHESIS; REVIEW; SECRETORY PATHWAY; UNFOLDED PROTEIN RESPONSE; CELL VACUOLE; CHEMISTRY; ENDOPLASMIC RETICULUM ASSOCIATED DEGRADATION; EXOCYTOSIS; GENETICS; GLYCOSYLATION; GOLGI COMPLEX; METABOLISM; PHYSIOLOGY; PICHIA; PROCEDURES; PROTEIN ENGINEERING; PROTEIN TRANSPORT;

ENDOPLASMIC RETICULUM-ASSOCIATED DEGRADATION; EXOCYTOSIS; GLYCOSYLATION; GOLGI APPARATUS; PICHIA; POLYSACCHARIDES; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN TRANSPORT; RECOMBINANT PROTEINS; UNFOLDED PROTEIN RESPONSE; VACUOLES;

EID: 84925491299     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-015-6470-z     Document Type: Review

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