In vivo synthesis of mammalian-like, hybrid-type N-glycans in Pichia pastoris

Applied and Environmental Microbiology

Volumn 70, Issue 5, 2004, Pages 2639-2646

  Vervecken, Wouter ^a   Kaigorodov, Vladimir ^a   Callewaert, Nico ^a,c   Geysens, Steven ^a   De Vusser, Kristof ^a   Contreras, Roland ^a,b  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^b Fundamental and Appl Molec Biology   (Belgium)

^c ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BLOOD; MICROBIOLOGY; OLIGOMERS; PROTEINS; STRUCTURE (COMPOSITION);

GLYCOSYLATION; HYPERMANNOSYLATION; MANNOSE REDUCTION;

CELLS;

ALPHA MANNOSIDASE; BETA 1,4 GALACTOSYLTRANSFERASE; GALACTOSYLTRANSFERASE; GLYCAN DERIVATIVE; GLYCOPROTEIN; MANNOSE; N ACETYLGLUCOSAMINYLTRANSFERASE; OLIGOSACCHARIDE; UNCLASSIFIED DRUG;

MICROBIOLOGY;

ARTICLE; CHEMICAL REACTION; CHEMICAL STRUCTURE; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; ENZYME ACTIVITY; FUNGAL STRAIN; GENE INACTIVATION; GENETIC ENGINEERING; GLYCOSYLATION; GOLGI COMPLEX; HETEROLOGOUS EXPRESSION; HYBRID; HYPERMANNOSYLATION; IN VIVO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN SECRETION; PROTEIN SYNTHESIS; SEQUENCE HOMOLOGY; SYNTHESIS; TECHNIQUE;

BIOTECHNOLOGY; ENDOPLASMIC RETICULUM; GALACTOSYLTRANSFERASES; GENETIC ENGINEERING; GLYCOSYLATION; GOLGI APPARATUS; HUMANS; MANNOSIDASES; N-ACETYLGLUCOSAMINYLTRANSFERASES; PICHIA; POLYSACCHARIDES; RECOMBINANT FUSION PROTEINS;

MAMMALIA; PICHIA; PICHIA PASTORIS;

EID: 2442661971     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.5.2639-2646.2004     Document Type: Article

References (33)

1. Ballou, C. E. 1990. Isolation, characterization, and properties of Saccharomyces cerevisiae mnn mutants with nonconditional protein glycosylation defects. Methods Enzymol. 185:440-470.
2. Bretthauer, R. K. 2003. Genetic engineering of Pichia pastoris to humanize N-glycosylation of proteins. Trends Biotechnol. 21:459-462.
3. Bretthauer, R. K., and F. J. Castellino. 1999. Glycosylation of Pichia pastoris-derived proteins. Biotechnol. Appl. Biochem. 30:193-200.
4. Callewaert, N., S. Geysens, F. Molemans, and R. Contreras. 2001. Ultrasensitive profiling and sequencing of N-linked oligosaccharides using standard DNA-sequencing equipment. Glycobiology 11:275-281.
5. Callewaert, N., W. Laroy, H. Cadirgi, S. Geysens, X. Saelens, W. Min Jou, and R. Contreras. 2001. Use of HDEL-tagged Trichoderma reesei mannosyl oligosaccharide 1,2-alpha-D-mannosidase for N-glycan engineering in Pichia pastoris. FEBS Lett. 503:173-178.
6. Cereghino, G. L., J. L. Cereghino, A. J. Sunga, M. A. Johnson, M. Lim, M. A. G. Gleeson, and J. M. Cregg. 2001. New selectable marker/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris. Gene 263:159-169.
7. Chen, X., P. R. Andreana, and P. G. Wang. 1999. Carbohydrates in transplantation. Curr. Opin. Chem. Biol. 3:650-658.
8. Chiba, Y., M. Suzuki, S. Yoshida, A. Yoshida, H. Ikenaga, M. Takeuchi, Y. Jigami, and E. Ichishima. 1998. Production of human compatible high mannose-type (Man5GlcNAc2) sugar chains in Saccharomyces cerevisiae. J. Biol. Chem. 273:26298-26304.
9. Chiba, Y., Y. Yamagata, S. Iijima, T. Nakajima, and E. Ichishima. 1993. The carbohydrate moiety of the acid carboxypeptidase from Aspergillus saitoi. Curr. Microbiol. 27:281-288.
10. Chitlaru, T., C. Kronman, M. Zeevi, M. Kam, A. Harel, A. Ordentlich, B. Velan, and A. Shafferman. 1998. Modulation of circulatory residence of recombinant acetylcholinesterase through biochemical or genetic manipulation of sialylation levels. Biochem. J. 336:647-658.
11. Choi, B. K., P. Bobrowicz, R. C. Davidson, S. R. Hamilton, D. H. Kung, H. Li, R. G. Miele, J. H. Nett, S. Wildt, and T. U. Gerngross. 2003. Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichia pastoris. Proc. Natl. Acad. Sci. USA. 100:5022-5027.
12. Cregg, J. M., J. L. Cereghino, J. Shi, and D. R. Higgins. 2000. Recombinant protein expression in Pichia pastoris. Mol. Biotechnol. 16:23-52.
13. Cregg, J. M., and K. A. Russell. 1998. Transformation, p. 27-39. In D. R. Higgins and J. M. Cregg (ed.), Pichia protocols, vol. 103. Humana Press, Totowa, N.J.
14. Dean, N. 1999. Asparagine-linked glycosylation in the yeast Golgi. Biochim. Biophys. Acta 1426:309-322.
15. Fukuda, M. 2000. Cell surface carbohydrates: cell type-specific expression, p. 12-61. In M. Fukuda and O. Hindgaul (ed.), Molecular and cellular glycobiology, vol. 30. Oxford University Press, New York, N.Y.
16. Hamilton, S. R., P. Bobrowicz, B. Bobrowicz, R. C. Davidson, H. Li, T. Mitchell, J. H. Nett, S. Rausch, T. A. Stadheim, H. Wischnewski, S. Wildt, and T. U. Gerngross. 2003. Production of complex human glycoproteins in yeast. Science 301:1244-1246.
17. Jackson, P. 1990. The use of polyacrylamide-gel electrophoresis for the high-resolution separation of reducing saccharides labelled with the fluorophore 8-aminonaphthalene-1,3,6-trisulphonic acid. Detection of picomolar quantities by an imaging system based on a cooled charge-coupled device. Biochem. J. 270:705-713.
18. Kornfeld, R., and S. Kornfeld. 1985. Assembly of asparagine-linked oligosaccharides. Annu. Rev. Biochem. 54:631-664.
19. Laroy, W., and R. Contreras. 2000. Cloning of Trypanosoma cruzi transsialidase and expression in Pichia pastoris. Protein Expr. Purif. 20:389-393.
20. Lee, S. J., S. Evers, D. Roeder, A. F. Parlow, J. Risteli, L. Risteli, Y. C. Lee, T. Feizi, H. Langen, and M. C. Nussenzweig. 2002. Mannose receptor-mediated regulation of serum glycoprotein homeostasis. Science 295:1898-1901.
21. Lussier, M., A. M. Sdicu, T. Ketela, and H. Bussey. 1995. Localization and targeting of the Saccharomyces cerevisiae Kre2p/Mnt1p alpha-1,2-mannosyltransferase to a medial-Golgi compartment. J. Cell Biol. 131:913-927.
22. Maras, M., N. Callewaert, K. Piens, M. Claeyssens, W. Martinet, S. Dewaele, H. Contreras, I. Dewerte, M. Penttila, and R. Contreras. 2000. Molecular cloning and enzymatic characterization of a Trichoderma reesei 1,2-alpha-D-mannosidase. J. Biotechnol. 77:255-263.
23. Maras, M., X. Saelens, W. Laroy, K. Piens, M. Claeyssens, W. Fiers, and R. Contreras. 1997. In vitro conversion of the carbohydrate moiety of fungal glycoproteins to mammalian-type oligosaccharides-evidence for N-acetyl-glucosaminyltransferase-I-accepting glycans from Trichoderma reesei. Eur. J. Biochem. 249:701-707.
24. Martinet, W., M. Maras, X. Saelens, W. M. Jou, and R. Contreras. 1998. Modification of the protein glycosylation pathway in the methylotrophic yeast Pichia pastoris. Bio/Technol. Lett. 20:1171-1177.
25. Murakami, K., and N. Sugio. 7 January 1997. Production of glycoprotein. Japan patent JP 1997000261-A1.
26. Roy, S. K., T. Yoko-o, H. Ikenaga, and Y. Jigami. 1998. Functional evidence for UDP-galactose transporter in Saccharomyces cerevisiae through the in vivo galactosylation and in vitro transport assay. J. Biol. Chem. 273:2583-2590.
27. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
28. Schwientek, T., H. Narimatsu, and J. F. Ernst. 1996. Golgi localization and in vivo activity of a mammalian glycosyltransferase (human beta-1,4-galactosyltransferase) in yeast. J. Biol. Chem. 271:3398-3405.
29. Van Petegem, F., H. Contreras, R. Contreras, and J. Van Beeumen. 2001. Trichoderma reesei alpha-1,2-mannosidase: structural basis for the cleavage of four consecutive mannose residues. J. Mol. Biol. 312:157-165.
30. Weigel, P. H. 1994. Galactosyl and N-acetylgalactosaminyl homeostasis: a function for mammalian asialoglycoprotein receptors. Bioessays 16:519-524.
31. Winkelhake, J. L., and G. L. Nicolson. 1976. Aglycosyl antibody. Effects of exoglycosidase treatments on autochthonous antibody survival time in the circulation. J. Biol. Chem. 251:1074-1080.
32. Yip, C. L., S. K. Welch, F. Klebl, T. Gilbert, P. Seidel, F. J. Grant, P. J. O'Hara, and V. L. MacKay. 1994. Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins. Proc. Natl. Acad. Sci. USA 91:2723-2727.
33. Yoshida, S., M. Suzuki, S. Yamano, M. Takeuchi, H. Ikenaga, N. Kioka, H. Sakai, and T. Komano. 1999. Expression and characterization of rat UDPN-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I in Saccharomyces cerevisiae. Glycobiology 9:53-58.