Pichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space-time yields for less complex proteins

Biotechnology Journal

Volumn 9, Issue 4, 2014, Pages 526-537

  Maccani, Andreas ^a,b   Landes, Nils ^a,b   Stadlmayr, Gerhard ^b   Maresch, Daniel ^b   Leitner, Christian ^b   Maurer, Michael ^a,c   Gasser, Brigitte ^a,b   Ernst, Wolfgang ^a,b   Kunert, Renate ^a,b   Mattanovich, Diethard ^a,b  

^a AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

^b UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^c Fachhochschule Campus Wien   (Austria)

Author keywords

CHO cells; Fed batch; Protein secretion; Recombinant protein production; Volumetric productivity

Indexed keywords

BATCH DATA PROCESSING; CELL CULTURE; CELLS; LEAD ACID BATTERIES; MACHINERY; PHYSIOLOGY; RECOMBINANT PROTEINS;

CHO CELL; FED BATCHES; PROTEIN SECRETION; RECOMBINANT PROTEIN PRODUCTIONS; VOLUMETRIC PRODUCTIVITY;

YEAST;

HUMAN SERUM ALBUMIN; RECOMBINANT PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANIMAL CELL; ARTICLE; BATCH PROCESS; CELL DENSITY; CHO CELL; COMPARATIVE STUDY; DOWNSTREAM PROCESSING; FUNGAL BIOMASS; FUNGAL STRAIN; GENE AMPLIFICATION; GENE EXPRESSION SYSTEM; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN SECRETION; SECRETION RATE; ANIMAL; BIOTECHNOLOGY; CHEMISTRY; CHO CELL LINE; CRICETULUS; HAMSTER; METABOLISM; PICHIA; PROCEDURES; SECRETION (PROCESS);

CRICETULUS GRISEUS; HUMAN IMMUNODEFICIENCY VIRUS 1; PICHIA PASTORIS;

ANIMALS; BIOTECHNOLOGY; CHO CELLS; CRICETINAE; CRICETULUS; PICHIA; RECOMBINANT PROTEINS; SINGLE-CHAIN ANTIBODIES;

EID: 84897446895     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201300305     Document Type: Article

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