메뉴 건너뛰기




Volumn 31, Issue 1, 2003, Pages 115-122

Production of the active antifungal Pisum sativum defensin 1 (Psd1) in Pichia pastoris: Overcoming the inefficiency of the STE13 protease

Author keywords

Antifungal protein; Circular dichroism; Heterologous expression; NMR; Pichia pastoris; Pisum sativum defensin

Indexed keywords

ASPERGILLUS; ASPERGILLUS NIGER; FUNGI; INSECTA; PICHIA; PICHIA PASTORIS; PISUM; PISUM SATIVUM; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0141650651     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(03)00136-0     Document Type: Article
Times cited : (95)

References (26)
  • 1
    • 0029347190 scopus 로고
    • Plant defensins: Novel antimicrobial peptides as components of the host defense system
    • W.F. Broekaert, F.R.G. Terras, B.P.A. Cammue, R.W. Osborn, Plant defensins: novel antimicrobial peptides as components of the host defense system, Plant Physiol. 108 (1995) 1353-1358.
    • (1995) Plant Physiol. , vol.108 , pp. 1353-1358
    • Broekaert, W.F.1    Terras, F.R.G.2    Cammue, B.P.A.3    Osborn, R.W.4
  • 4
    • 0031465590 scopus 로고    scopus 로고
    • Specific, high affinity binding sites for an antifungal plant defensin on Neurospora crassa hyphae and microsomal membranes
    • K. Thevissen, R.W. Osborn, D.P. Ackland, W.F. Broekaert, Specific, high affinity binding sites for an antifungal plant defensin on Neurospora crassa hyphae and microsomal membranes, J. Biol. Chem. 272 (1997) 32176-32181.
    • (1997) J. Biol. Chem. , vol.272 , pp. 32176-32181
    • Thevissen, K.1    Osborn, R.W.2    Ackland, D.P.3    Broekaert, W.F.4
  • 7
    • 6544244002 scopus 로고    scopus 로고
    • The promoter of the plant defensin gene PDF1.2 from Arabidopsis is systemically activated by fungal pathogens and responds to methyl jasmonate but not to salicylic acid
    • J.M. Manners, I.A. Penninckx, K. Vermaere, K. Kazan, R.L. Brown, A. Morgan, D.J. Maclean, M.D. Curtis, B.P. Cammue. W.F. Broekaert, The promoter of the plant defensin gene PDF1.2 from Arabidopsis is systemically activated by fungal pathogens and responds to methyl jasmonate but not to salicylic acid, Plant Mol. Biol. 6 (1998) 1071-1080.
    • (1998) Plant Mol. Biol. , vol.6 , pp. 1071-1080
    • Manners, J.M.1    Penninckx, I.A.2    Vermaere, K.3    Kazan, K.4    Brown, R.L.5    Morgan, A.6    Maclean, D.J.7    Curtis, M.D.8    Cammue, B.P.9    Broekaert, W.F.10
  • 9
    • 0036301039 scopus 로고    scopus 로고
    • Solution structure of Pisum sativum defensin 1 by high resolution NMR: Plant defensins, identical backbone with different mechanisms of action
    • M.S. Almeida, K.M. Cabral, E. Kurtenbach, F.C.L. Almeida, A.P. Valente, Solution structure of Pisum sativum defensin 1 by high resolution NMR: plant defensins, identical backbone with different mechanisms of action, J. Mol. Biol. 315 (2002) 749-757.
    • (2002) J. Mol. Biol. , vol.315 , pp. 749-757
    • Almeida, M.S.1    Cabral, K.M.2    Kurtenbach, E.3    Almeida, F.C.L.4    Valente, A.P.5
  • 13
    • 0033955337 scopus 로고    scopus 로고
    • Heterologous protein expression in the methylotrophic yeast Pichia pastoris
    • J.L. Cereghino, J.M. Cregg, Heterologous protein expression in the methylotrophic yeast Pichia pastoris, FEMS Microb. Rev. 24 (2000) 45-66.
    • (2000) FEMS Microb. Rev. , vol.24 , pp. 45-66
    • Cereghino, J.L.1    Cregg, J.M.2
  • 14
  • 15
    • 0028326048 scopus 로고
    • Isolation of intact DNA and RNA from plant tissues
    • R. Soni, J.A.H. Murray, Isolation of intact DNA and RNA from plant tissues, Anal. Biochem. 218 (1994) 474-476.
    • (1994) Anal. Biochem. , vol.218 , pp. 474-476
    • Soni, R.1    Murray, J.A.H.2
  • 16
  • 17
    • 0026183530 scopus 로고
    • The Fusarium solani-induced expression of a pea gene family encoding high cysteine content proteins
    • C.C. Chiang, L.A. Hadwiger, The Fusarium solani-induced expression of a pea gene family encoding high cysteine content proteins, Mol. Plant Microbe. Interact. 4 (1991) 324-331.
    • (1991) Mol. Plant Microbe. Interact. , vol.4 , pp. 324-331
    • Chiang, C.C.1    Hadwiger, L.A.2
  • 18
    • 0001001711 scopus 로고
    • cDNA sequences for pea disease resistance response genes
    • B. Fristensky, D. Horovitz, L.A. Hadwiger, cDNA sequences for pea disease resistance response genes, Plant Mol. Biol. 11 (1988) 713-715.
    • (1988) Plant Mol. Biol. , vol.11 , pp. 713-715
    • Fristensky, B.1    Horovitz, D.2    Hadwiger, L.A.3
  • 20
    • 0014949207 scopus 로고
    • Cleavage of structrual proteins during the assembly of the head of bacteriophage T4
    • U.K. Laemmli, Cleavage of structrual proteins during the assembly of the head of bacteriophage T4, Nature 227 (1970) 680-685.
    • (1970) Nature , vol.227 , pp. 680-685
    • Laemmli, U.K.1
  • 21
    • 45249127991 scopus 로고
    • Rapid recording of 2D NMR spectra without phase cycling-application to the study of hydrogen exchange in proteins
    • D. Marion, M. Ikura, R. Tschudin, A. Bax, Rapid recording of 2D NMR spectra without phase cycling-application to the study of hydrogen exchange in proteins, J. Magn. Reson. 85 (1989) 393-399.
    • (1989) J. Magn. Reson. , vol.85 , pp. 393-399
    • Marion, D.1    Ikura, M.2    Tschudin, R.3    Bax, A.4
  • 22
    • 13444269041 scopus 로고
    • Computer-optimized decoupling scheme for wideband applications and low-level operation
    • A.J. Shaka, P.B. Barker, R. Freeman, Computer-optimized decoupling scheme for wideband applications and low-level operation, J. Magn. Reson. 64 (1985) 547-552.
    • (1985) J. Magn. Reson. , vol.64 , pp. 547-552
    • Shaka, A.J.1    Barker, P.B.2    Freeman, R.3
  • 23
    • 0026951903 scopus 로고
    • Gradient-tailored excitation for single-quantum NMR-spectroscopy of aqueous solutions
    • M. Piotto, V. Saudek, V. Sklenar, Gradient-tailored excitation for single-quantum NMR-spectroscopy of aqueous solutions, J. Biomol. NMR 2 (1992) 661-666.
    • (1992) J. Biomol. NMR , vol.2 , pp. 661-666
    • Piotto, M.1    Saudek, V.2    Sklenar, V.3
  • 24
    • 0018416496 scopus 로고
    • Ellman's reagent: 5,5′-dithiobis(2-nitrobenzoic acid) - A reexamination
    • P.W. Riddles, R.L. Blakeley, B. Zerner, Ellman's reagent: 5,5′-dithiobis(2-nitrobenzoic acid) - a reexamination, Anal. Biochem. 94 (1979) 75-81.
    • (1979) Anal. Biochem. , vol.94 , pp. 75-81
    • Riddles, P.W.1    Blakeley, R.L.2    Zerner, B.3
  • 26
    • 0034923785 scopus 로고    scopus 로고
    • 13C isotopic labeling of proteins expressed in Pichia pastoris
    • 13C isotopic labeling of proteins expressed in Pichia pastoris, J. Biochem. 130 (2001) 19-22.
    • (2001) J. Biochem. , vol.130 , pp. 19-22
    • Rodriguez, E.1    Krishna, N.R.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.